SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015732
Component protein type	TssE
T6SS ID (Type)	T6SS01261 (Type i5)
Strain	Rhizobium etli bv. mimosae str. Mim1
Replicon	plasmid pRetMIM1f
Sequence	Protein sequence (170 a.a.); Nucleotide sequence (510 bp)
Reference	[1] Salinero-Lanzarote A, Pacheco-Moreno A, Domingo-Serrano L, et al. The Type VI secretion system of Rhizobium etli Mim1 has a positive effect in symbiosis. FEMS Microbiol Ecol. 2019 May 1;95(5). pii: fiz054. doi: 10.1093/femsec/fiz054. PMID: 30977796

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	REMIM1_RS31775
Coordinate (Strand)	501480..501989 (-)
NCBI ID	WP_020919505.1
RefSeq	NC_021911
Uniprot ID	S5S8B4_RHIET
KEGG ID	rel:REMIM1_PF00470
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	4.2e-13	44..142

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-169	MADPLERYRQRDRVLARSILDRLIDEAPDRAADPPMNFVDQVREMREAIRRDLEALLNTRRCPTTPPAVLAELKDALVSYGVDGVVSANLMTDEAKLRLAAAIERRIALFETRLSDVRVTILKSRTITERALRLRIQATFRLHEGMPPISFESTIDPSTQRFLVEAAHG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9914	-	-

Protein sequence: 170 a.a.

						>T6CP015732 NC_021911:c501989-501480 [Rhizobium etli bv. mimosae str. Mim1] [TssE]
MADPLERYRQRDRVLARSILDRLIDEAPDRAADPPMNFVDQVREMREAIRRDLEALLNTRRCPTTPPAVLAELKDALVSY
GVDGVVSANLMTDEAKLRLAAAIERRIALFETRLSDVRVTILKSRTITERALRLRIQATFRLHEGMPPISFESTIDPSTQ
RFLVEAAHG*					

Nucleotide sequence: 510 bp

						>T6CP015732 NC_021911:c501989-501480 [Rhizobium etli bv. mimosae str. Mim1] [TssE]
ATGGCTGATCCGCTCGAACGCTACCGGCAGCGCGACCGCGTCCTGGCCCGCTCGATCCTCGACAGGCTGATCGACGAGGC
CCCGGATCGCGCTGCCGATCCGCCGATGAACTTCGTCGATCAGGTTCGCGAGATGCGCGAGGCGATCCGCCGCGATCTCG
AGGCGCTGCTCAACACGCGCCGCTGCCCGACCACGCCGCCGGCCGTCCTTGCCGAACTGAAGGATGCTCTGGTGAGCTAC
GGCGTCGACGGCGTCGTCTCGGCCAATCTGATGACCGACGAAGCGAAGCTCAGGCTGGCGGCCGCAATCGAGCGGCGGAT
CGCGCTCTTCGAAACGCGGCTTTCCGATGTCAGGGTGACGATCCTGAAAAGCCGGACGATAACGGAGCGGGCGCTGCGCC
TGCGCATCCAGGCGACCTTCCGCCTGCATGAAGGCATGCCGCCGATCAGCTTCGAATCGACAATCGACCCTTCCACCCAG
CGTTTCCTGGTGGAGGCCGCGCATGGCTGA					