Detailed information of component protein


Summary

Component ID T6CP015731
Component protein type TssF
T6SS ID (Type) T6SS01261 experimental (Type i5)
Strain Rhizobium etli bv. mimosae str. Mim1
Replicon plasmid pRetMIM1f
Sequence Protein sequence (594 a.a.); Nucleotide sequence (1782 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) REMIM1_RS31770
Coordinate (Strand) 499706..501487 (-)
NCBI ID WP_020919504.1
RefSeq NC_021911
Uniprot ID S5SXD1_RHIET
KEGG ID rel:REMIM1_PF00469
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 4.6e-202 1..590
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-593 MAEGFLHRYNEELLALRRRAARFADAFPKIAGRLRMTGDVADDPHVERLIQSFAYSAARVRQKLDDEFPELTDSLLETLYPHYLAPLPSMSIVKFRSSDTLASAQTVPRHTEILAETVRGEACRFRTTQDVKIAPIEITGATLSGQPINAPLSAYAGVAGCLRLSIRSLDARKPFGSLGVNRLRLHIPSPWQQAVAIYELLANHTLGIALARHADDRSPVFLPARNLKPVGFEPEEAMLPYPAPSFTGYRLLTEFFALPRKFLFLDIDGLDAWKGETLELFIYLRESDAKLEREVSVRDFTLNATPVINLFRQVCEPVAIDGTRTEYRLMPDARRQKTREIYSIDRVLLTGSRGQEEFCQPFFGRSQRAGTSATYWQAYRRFDEDDRTSDMDIAFVDRQRRSAGPVDAVASVDTLCLNRDLPEQLPFGGGHPFLQLASGHEAVAGVEALIPPTPSVRMYDHDGRNWRLISHLVLNHLSLFDNGGAALKDILSLYAFRDSPETKQLVDALVRIKATNSTARLGSGGMVPGTEITLEFDPAAIDRPSAFLFGSLLDRFFSLYTSVNSFTRLTVGMKGQSRPIAQWPARAAERPLL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9867 - -


  Protein sequence: 594 a.a.    

>T6CP015731 NC_021911:c501487-499706 [Rhizobium etli bv. mimosae str. Mim1] [TssF]
MAEGFLHRYNEELLALRRRAARFADAFPKIAGRLRMTGDVADDPHVERLIQSFAYSAARVRQKLDDEFPELTDSLLETLY
PHYLAPLPSMSIVKFRSSDTLASAQTVPRHTEILAETVRGEACRFRTTQDVKIAPIEITGATLSGQPINAPLSAYAGVAG
CLRLSIRSLDARKPFGSLGVNRLRLHIPSPWQQAVAIYELLANHTLGIALARHADDRSPVFLPARNLKPVGFEPEEAMLP
YPAPSFTGYRLLTEFFALPRKFLFLDIDGLDAWKGETLELFIYLRESDAKLEREVSVRDFTLNATPVINLFRQVCEPVAI
DGTRTEYRLMPDARRQKTREIYSIDRVLLTGSRGQEEFCQPFFGRSQRAGTSATYWQAYRRFDEDDRTSDMDIAFVDRQR
RSAGPVDAVASVDTLCLNRDLPEQLPFGGGHPFLQLASGHEAVAGVEALIPPTPSVRMYDHDGRNWRLISHLVLNHLSLF
DNGGAALKDILSLYAFRDSPETKQLVDALVRIKATNSTARLGSGGMVPGTEITLEFDPAAIDRPSAFLFGSLLDRFFSLY
TSVNSFTRLTVGMKGQSRPIAQWPARAAERPLL*

  Nucleotide sequence: 1782 bp    

>T6CP015731 NC_021911:c501487-499706 [Rhizobium etli bv. mimosae str. Mim1] [TssF]
ATGGCTGAGGGCTTCCTCCACCGTTATAATGAGGAATTGCTGGCGCTGCGCCGCCGGGCCGCGCGCTTTGCCGATGCCTT
CCCGAAGATCGCCGGCCGCCTGCGCATGACCGGCGACGTCGCCGACGACCCGCATGTGGAGCGGCTGATCCAGAGCTTCG
CCTATTCGGCCGCGCGCGTTCGCCAGAAGCTCGACGACGAGTTCCCGGAGCTGACCGACAGTCTGCTCGAAACGCTCTAT
CCGCATTACCTCGCGCCCCTTCCATCCATGAGCATCGTGAAGTTCCGGTCGAGCGATACGCTGGCCTCGGCGCAGACCGT
GCCGCGCCACACCGAGATCCTGGCCGAAACGGTGCGCGGCGAAGCCTGCCGCTTCCGCACGACGCAGGATGTGAAAATCG
CGCCGATCGAAATTACGGGCGCCACCCTTTCCGGCCAGCCGATCAATGCACCGCTTTCGGCCTATGCCGGCGTTGCCGGC
TGCCTCAGGCTCTCCATCCGCAGCCTCGATGCCCGCAAGCCCTTCGGAAGCCTCGGCGTAAACCGGCTGCGCCTGCACAT
TCCCTCCCCCTGGCAGCAGGCCGTGGCGATCTACGAGCTGCTGGCCAATCACACGCTCGGCATCGCGCTTGCCAGGCATG
CGGACGATCGTTCGCCGGTCTTCCTGCCGGCCCGGAATCTCAAGCCTGTCGGCTTCGAGCCCGAAGAGGCGATGCTGCCC
TATCCGGCGCCGAGCTTCACCGGCTACCGGCTGCTCACCGAATTCTTCGCCCTGCCCAGAAAGTTTCTGTTTCTCGACAT
CGATGGGCTCGATGCCTGGAAGGGCGAGACGCTGGAGCTCTTCATCTATCTCAGGGAGAGTGACGCCAAGCTGGAGCGGG
AGGTGTCGGTTCGCGATTTCACGCTCAACGCCACGCCCGTCATCAATCTCTTCCGCCAGGTCTGCGAACCGGTCGCCATC
GACGGCACCCGCACTGAATATCGCCTCATGCCCGATGCGCGGCGCCAGAAGACCCGCGAGATCTATTCGATCGACCGCGT
GCTGCTGACCGGATCACGCGGCCAGGAGGAATTCTGCCAGCCCTTCTTCGGCCGGTCGCAGCGGGCGGGCACGAGCGCGA
CCTACTGGCAGGCCTATCGCCGCTTCGACGAGGATGACAGGACCAGCGACATGGACATCGCCTTCGTCGACAGGCAGCGC
CGCTCCGCAGGTCCCGTCGATGCGGTCGCGAGCGTCGATACGCTCTGCCTCAACCGCGACCTGCCGGAACAGCTTCCCTT
CGGCGGCGGCCATCCCTTCCTGCAGCTTGCCTCCGGCCATGAGGCGGTGGCCGGCGTCGAGGCGCTGATCCCGCCGACGC
CTTCGGTGCGAATGTATGATCACGATGGCCGGAACTGGCGGCTGATCTCGCATCTCGTCCTCAACCATCTCTCGCTCTTC
GACAATGGCGGCGCGGCGCTGAAAGATATCCTGTCGCTCTACGCCTTCCGCGACAGCCCCGAGACCAAGCAATTGGTCGA
CGCGCTGGTGCGGATCAAGGCCACCAATTCGACGGCCCGTCTCGGGAGCGGCGGCATGGTGCCCGGCACCGAGATCACGC
TGGAATTCGATCCGGCCGCCATCGACCGGCCCTCGGCCTTCCTGTTCGGCAGCCTGCTCGACCGCTTCTTCAGCCTCTAC
ACCTCGGTCAACAGCTTCACCCGGCTCACCGTCGGCATGAAGGGTCAGTCGAGACCGATCGCCCAATGGCCGGCGCGCGC
CGCCGAGCGCCCGCTTCTTTGA