SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015730
Component protein type	TssG
T6SS ID (Type)	T6SS01261 (Type i5)
Strain	Rhizobium etli bv. mimosae str. Mim1
Replicon	plasmid pRetMIM1f
Sequence	Protein sequence (334 a.a.); Nucleotide sequence (1002 bp)
Reference	[1] Salinero-Lanzarote A, Pacheco-Moreno A, Domingo-Serrano L, et al. The Type VI secretion system of Rhizobium etli Mim1 has a positive effect in symbiosis. FEMS Microbiol Ecol. 2019 May 1;95(5). pii: fiz054. doi: 10.1093/femsec/fiz054. PMID: 30977796

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	REMIM1_RS31765
Coordinate (Strand)	498684..499685 (-)
NCBI ID	WP_020919503.1
RefSeq	NC_021911
Uniprot ID	S5S7X1_RHIET
KEGG ID	rel:REMIM1_PF00468
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	3e-66	42..309

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-333	MDMQAASKPDALTDLLERDPGRFEPVTAFRVAQASVGEGRLDVASHVGVSPARLAVSGYKRKGGRATVRSALAGLVGALGSMPSAYNELIMREERNRSRALAGFFDLFGARMAELFADASEKYRIARRLRWGGTWASNAFVTTLLSLTGFGTKRLVEKSGVDEELILRFSGFFAARNRNAVNLRAMLTEFSGLPVEIELFRGRWLTIPMEERSRMGQPQGVQLGVNATAGAAIHDFSGGFRIVIGPLGYADYLSLAPGGSAITELFALARLYVGAALEFDIQVILKKEDVPFCRLGQGGDPPRLGWNSWARVAPAAKDSGDAVIVERPPAEMR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9641	-	-

Protein sequence: 334 a.a.

						>T6CP015730 NC_021911:c499685-498684 [Rhizobium etli bv. mimosae str. Mim1] [TssG]
MDMQAASKPDALTDLLERDPGRFEPVTAFRVAQASVGEGRLDVASHVGVSPARLAVSGYKRKGGRATVRSALAGLVGALG
SMPSAYNELIMREERNRSRALAGFFDLFGARMAELFADASEKYRIARRLRWGGTWASNAFVTTLLSLTGFGTKRLVEKSG
VDEELILRFSGFFAARNRNAVNLRAMLTEFSGLPVEIELFRGRWLTIPMEERSRMGQPQGVQLGVNATAGAAIHDFSGGF
RIVIGPLGYADYLSLAPGGSAITELFALARLYVGAALEFDIQVILKKEDVPFCRLGQGGDPPRLGWNSWARVAPAAKDSG
DAVIVERPPAEMR*					

Nucleotide sequence: 1002 bp

						>T6CP015730 NC_021911:c499685-498684 [Rhizobium etli bv. mimosae str. Mim1] [TssG]
ATGGATATGCAAGCCGCCAGCAAGCCAGATGCGCTGACCGACCTGCTCGAACGCGATCCCGGCCGCTTCGAACCGGTGAC
GGCTTTCCGCGTCGCGCAGGCGTCGGTCGGCGAAGGACGGCTTGACGTCGCCTCCCATGTCGGCGTCTCGCCGGCGCGGC
TTGCGGTCAGCGGATACAAGCGCAAGGGAGGCCGCGCTACCGTGCGCAGCGCGCTTGCCGGCCTCGTCGGCGCCCTCGGT
TCGATGCCTTCAGCCTATAACGAACTCATCATGCGCGAGGAGCGCAACCGGTCGCGGGCGCTGGCGGGCTTCTTCGATCT
CTTCGGTGCGCGCATGGCCGAACTTTTCGCCGATGCCAGTGAGAAATACCGCATCGCCCGGCGGCTGCGCTGGGGCGGCA
CATGGGCGAGCAATGCCTTCGTCACCACGCTGCTCTCGCTCACCGGCTTCGGCACGAAGCGCCTCGTCGAAAAGAGCGGC
GTCGACGAGGAGCTGATCCTGCGTTTTTCCGGCTTCTTCGCCGCACGCAACCGCAATGCCGTCAATCTGCGGGCGATGCT
GACCGAATTCAGCGGCCTGCCGGTGGAGATCGAATTGTTCCGCGGCCGCTGGCTCACCATCCCGATGGAGGAGCGCAGCC
GCATGGGCCAGCCGCAGGGCGTGCAACTCGGCGTTAATGCGACGGCGGGTGCTGCCATCCACGATTTCAGCGGCGGTTTT
CGCATCGTCATCGGTCCGCTCGGCTACGCCGACTACCTCTCGCTGGCGCCGGGCGGGTCGGCGATCACCGAACTCTTCGC
CCTGGCACGGCTCTATGTCGGCGCAGCACTCGAATTCGACATTCAGGTCATCCTGAAGAAGGAGGACGTTCCCTTCTGCC
GGCTCGGCCAGGGGGGCGATCCACCGCGCCTGGGCTGGAACAGCTGGGCGCGGGTCGCGCCGGCGGCGAAAGACAGCGGC
GACGCGGTGATCGTCGAGCGCCCGCCGGCCGAGATGCGGTGA					