Detailed information of component protein


Summary

Component ID T6CP015721
Component protein type TssM
T6SS ID (Type) T6SS01254 experimental (Type i1)
Strain Vibrio parahaemolyticus strain 12-009A/1335
Replicon chromosome
Sequence Protein sequence (1130 a.a.); Nucleotide sequence (3390 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) B5C29_RS17135
Coordinate (Strand) 30119..33508 (-)
NCBI ID 1189147607
RefSeq NZ_MYFF01000478
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1.2e-54 154..408
IcmF-related PF06761.14 5.8e-42 496..755
IcmF_C PF06744.14 1.4e-18 1004..1107
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-11 MFKQKILRNPV
Transmembrane helix 12-34 VVSTIVALVLAAIFAAVYLFILE
Outside 35-38 DADK
Transmembrane helix 39-61 LYLWVGLGVITGFYFTFQLSLWL
Inside 62-401 KKKNSSKVQRLETEEEALSMVIRPLLSNAGKKPIYLMVGNKGAGRRQFLFNSSAIKPMDRSRTAKNDFFEWYESDGAVYVKPDQRLIFQEVSSSDASLWNTFIDEVIRHRPRKPFSGCLFFVDFEFMIVSEPEQKDYTMTALLQRLNSITEKTSSALPVYLMMTKLDKLDGFKEYVHSSSLKTRIEFLSIPLKEAKGVLTEYYLDSFHNLVKVLESNALDASANSTDINEKQAILAFPKQFELCQREISYVLERLCDTNNGVYSLDIREIFFCSSLQGGRKYNLLAKSCSNYFNLPIIASEHTQLTETPYFSRFLLDSQILPESDFAGENKTYLRMIQRQ
Transmembrane helix 402-424 SHLAMLASVVLLAGGTYFFVTTL
Outside 425-1129 DSNLRVINQLLNIDEKVQTEQEASFNQLLQSATRAIQPSYNAWLEGSRALDEEVIPLNISRLNESTKLAYEALIKEVAQQLMPVIEQGYRFELTHNQHDVTATLPLLKGYLMLKDPTKRDIQFLRRQTLQTLTDLSDKPDVVNQAMRYLDAYFRTQFSPVDINMDVVRATRRSLLANSNVDLVYAGILNQADAIDLGTLDLQRAVGFEFNNVFNEPLDDNRLVIDKIYTSTGFSTFYRPRVDLMSKQVISDNWVLGLSSYVIPTKEEQESFKNQVRKKYTDDYINYWRNALSELKIQSYSNVGDLTNAIDLISGPSSPMTTVLKQVYVNTQFSPVGEKNVLISKVDPKLAEVANSASEAVEEVVQPDYLLMKRVEQAFHLLNQLQVSETPNSPTPWDETIAALSRVRTYMKDIADAPDPQMAALAAAQHRMSSSEADPLIKLKQIAQKSPEPVRSWLLDVVKQSWSVMITESAKGIQTQWYSEIYTKFKEIGLGKYPFDLTATEEISIEDFELLFASGGLLDTFIQKNFAPFYDTNLWVPKQVDGETMPLSSALLVQLRNYNVIRDTLINKSTNRVYIPFSAKVLDLDSSAIRANLKISDTYINYYHGPSRIREMEWPPQNGDFNISITIQDVTDEGKQHVLNKSGQWAIYRLLGDSTLTNTHNGSFVSDIKVSGRDLSLRITPLTQKNPFTLAELYNFTLPESI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9980 - -


  Protein sequence: 1130 a.a.    

>T6CP015721 NZ_MYFF01000478:c33508-30119 [Vibrio parahaemolyticus] [TssM]
MFKQKILRNPVVVSTIVALVLAAIFAAVYLFILEDADKLYLWVGLGVITGFYFTFQLSLWLKKKNSSKVQRLETEEEALS
MVIRPLLSNAGKKPIYLMVGNKGAGRRQFLFNSSAIKPMDRSRTAKNDFFEWYESDGAVYVKPDQRLIFQEVSSSDASLW
NTFIDEVIRHRPRKPFSGCLFFVDFEFMIVSEPEQKDYTMTALLQRLNSITEKTSSALPVYLMMTKLDKLDGFKEYVHSS
SLKTRIEFLSIPLKEAKGVLTEYYLDSFHNLVKVLESNALDASANSTDINEKQAILAFPKQFELCQREISYVLERLCDTN
NGVYSLDIREIFFCSSLQGGRKYNLLAKSCSNYFNLPIIASEHTQLTETPYFSRFLLDSQILPESDFAGENKTYLRMIQR
QSHLAMLASVVLLAGGTYFFVTTLDSNLRVINQLLNIDEKVQTEQEASFNQLLQSATRAIQPSYNAWLEGSRALDEEVIP
LNISRLNESTKLAYEALIKEVAQQLMPVIEQGYRFELTHNQHDVTATLPLLKGYLMLKDPTKRDIQFLRRQTLQTLTDLS
DKPDVVNQAMRYLDAYFRTQFSPVDINMDVVRATRRSLLANSNVDLVYAGILNQADAIDLGTLDLQRAVGFEFNNVFNEP
LDDNRLVIDKIYTSTGFSTFYRPRVDLMSKQVISDNWVLGLSSYVIPTKEEQESFKNQVRKKYTDDYINYWRNALSELKI
QSYSNVGDLTNAIDLISGPSSPMTTVLKQVYVNTQFSPVGEKNVLISKVDPKLAEVANSASEAVEEVVQPDYLLMKRVEQ
AFHLLNQLQVSETPNSPTPWDETIAALSRVRTYMKDIADAPDPQMAALAAAQHRMSSSEADPLIKLKQIAQKSPEPVRSW
LLDVVKQSWSVMITESAKGIQTQWYSEIYTKFKEIGLGKYPFDLTATEEISIEDFELLFASGGLLDTFIQKNFAPFYDTN
LWVPKQVDGETMPLSSALLVQLRNYNVIRDTLINKSTNRVYIPFSAKVLDLDSSAIRANLKISDTYINYYHGPSRIREME
WPPQNGDFNISITIQDVTDEGKQHVLNKSGQWAIYRLLGDSTLTNTHNGSFVSDIKVSGRDLSLRITPLTQKNPFTLAEL
YNFTLPESI*

  Nucleotide sequence: 3390 bp    

>T6CP015721 NZ_MYFF01000478:c33508-30119 [Vibrio parahaemolyticus] [TssM]
ATGTTCAAACAAAAAATACTAAGAAATCCAGTCGTGGTTTCTACCATAGTGGCTTTGGTATTAGCCGCGATATTTGCAGC
GGTATATTTATTCATACTTGAAGACGCAGATAAGCTTTATCTATGGGTTGGTTTAGGAGTTATTACTGGTTTTTATTTTA
CATTCCAGCTTTCCCTATGGCTTAAGAAAAAAAACAGCTCAAAAGTTCAACGTTTAGAGACGGAAGAAGAAGCTCTTTCA
ATGGTGATCCGCCCTCTTCTTTCTAATGCGGGCAAAAAGCCAATCTATCTGATGGTTGGTAATAAAGGGGCCGGTCGACG
TCAGTTTTTGTTTAATTCCAGCGCAATTAAGCCGATGGACCGTTCTCGCACAGCAAAAAACGATTTCTTCGAATGGTATG
AATCTGATGGTGCCGTTTATGTTAAGCCAGATCAACGCCTCATCTTCCAGGAAGTTTCAAGCAGTGATGCTTCTCTCTGG
AATACTTTTATTGATGAAGTGATTCGTCATCGTCCGAGAAAACCCTTTTCAGGTTGCTTATTCTTTGTTGATTTCGAATT
CATGATTGTATCTGAGCCAGAGCAGAAAGATTACACCATGACAGCGCTATTGCAGCGTTTGAACTCTATCACTGAGAAGA
CCTCGTCTGCACTACCTGTTTATTTGATGATGACGAAACTCGACAAACTAGATGGCTTTAAAGAATACGTTCACTCTAGT
TCACTAAAAACACGTATTGAATTTCTGTCCATTCCATTGAAGGAAGCGAAAGGTGTACTTACCGAATACTACCTAGATAG
CTTCCATAATCTGGTAAAAGTGCTAGAAAGCAACGCTTTAGACGCATCGGCAAACTCAACAGATATCAACGAAAAGCAAG
CCATCCTTGCCTTCCCAAAACAGTTTGAACTCTGTCAACGTGAAATCAGCTACGTGCTTGAACGTCTTTGCGATACAAAC
AATGGCGTTTACTCGTTGGATATTCGCGAAATCTTCTTCTGTTCTTCTTTGCAAGGTGGACGCAAGTACAACTTGTTAGC
CAAAAGCTGCAGTAATTACTTCAATTTACCAATTATCGCATCTGAGCACACGCAACTAACTGAAACACCGTACTTTTCTC
GCTTCTTGCTTGACTCTCAAATCCTCCCAGAGAGTGACTTCGCAGGCGAGAACAAGACTTACCTACGCATGATTCAGCGT
CAAAGCCATTTGGCAATGCTGGCATCCGTTGTCTTATTGGCAGGCGGTACTTATTTTTTTGTCACAACACTAGACAGTAA
CTTGCGTGTTATTAACCAATTGCTGAATATCGACGAAAAAGTTCAAACAGAACAAGAAGCAAGCTTCAACCAGTTATTGC
AGAGTGCTACTCGTGCAATCCAACCCTCATACAATGCTTGGTTAGAAGGATCACGAGCATTAGATGAAGAAGTAATACCG
CTAAATATAAGCAGGTTAAACGAAAGTACAAAATTAGCGTATGAAGCATTGATTAAAGAGGTTGCTCAACAACTTATGCC
TGTTATTGAGCAAGGCTACCGTTTTGAACTGACACACAACCAACATGATGTCACTGCAACGTTGCCGTTACTAAAAGGCT
ACTTGATGTTGAAGGATCCGACCAAGCGAGATATTCAGTTTTTACGACGCCAAACATTGCAAACACTGACAGACTTGAGC
GATAAGCCGGATGTGGTAAATCAAGCTATGCGATACTTGGACGCCTACTTCCGCACTCAATTCTCACCTGTAGATATCAA
CATGGATGTCGTTCGAGCAACTCGTCGCTCATTGTTGGCAAATTCAAATGTGGATCTTGTTTACGCAGGCATTTTAAACC
AAGCAGATGCTATCGATCTTGGCACACTCGATCTACAACGTGCAGTTGGCTTTGAATTTAACAACGTATTTAACGAGCCG
TTAGATGACAACCGACTAGTAATCGATAAGATTTATACCTCCACTGGCTTTAGTACCTTCTATCGCCCTCGCGTCGACTT
GATGTCTAAACAAGTAATCAGTGATAACTGGGTGCTAGGATTGTCCAGCTATGTTATCCCAACCAAAGAAGAGCAAGAAT
CGTTTAAAAATCAAGTACGTAAAAAGTACACCGATGATTATATCAACTACTGGCGCAACGCTCTCAGTGAGTTAAAAATT
CAGAGTTATAGCAATGTGGGTGATCTAACAAATGCTATCGACCTAATCTCTGGACCATCTTCACCAATGACGACTGTATT
AAAGCAGGTCTATGTAAATACTCAGTTTTCACCGGTAGGAGAAAAAAACGTACTCATCTCGAAAGTAGACCCAAAACTGG
CAGAAGTGGCGAACTCGGCGTCAGAAGCGGTTGAAGAAGTGGTTCAGCCGGATTACTTGTTGATGAAGCGTGTAGAACAA
GCGTTCCATCTTCTAAATCAGCTTCAAGTAAGCGAGACTCCTAACTCCCCTACTCCATGGGACGAAACTATCGCAGCTTT
AAGCCGAGTTCGTACCTACATGAAAGATATTGCGGATGCTCCCGATCCACAAATGGCAGCACTTGCAGCCGCTCAACACC
GAATGAGCAGCTCTGAAGCCGATCCGCTAATTAAACTTAAACAGATTGCTCAGAAGTCACCAGAGCCAGTACGCAGTTGG
TTACTTGATGTCGTAAAGCAAAGCTGGTCGGTAATGATCACCGAATCAGCAAAAGGCATTCAAACCCAATGGTATAGTGA
AATTTACACCAAATTTAAAGAGATAGGACTTGGTAAATACCCATTTGATCTCACAGCAACAGAGGAGATTTCTATCGAAG
ACTTCGAACTGTTGTTTGCTTCAGGTGGATTGTTAGACACATTTATTCAGAAAAACTTCGCACCGTTCTACGATACCAAC
TTGTGGGTACCAAAACAGGTGGATGGTGAAACAATGCCACTATCTTCAGCCTTACTGGTGCAATTGCGCAATTACAACGT
TATCCGCGACACGCTGATCAATAAGAGTACTAACCGAGTTTATATTCCATTCAGTGCGAAAGTATTAGATCTTGACTCAA
GTGCTATCAGAGCCAATTTGAAAATCTCTGACACTTATATCAACTACTATCACGGTCCGAGCCGAATTCGTGAAATGGAG
TGGCCACCGCAAAACGGCGACTTCAACATTAGTATCACGATCCAAGATGTGACAGACGAAGGTAAACAACACGTTTTAAA
TAAGAGTGGTCAATGGGCGATATATAGATTGCTAGGTGATTCGACCCTGACAAACACTCATAACGGTAGCTTCGTTAGTG
ACATAAAAGTATCAGGGAGGGATTTGAGCCTCCGTATAACTCCCTTGACACAAAAAAACCCTTTCACTTTGGCTGAGTTG
TACAACTTTACGCTCCCAGAGTCGATTTGA