SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015719
Component protein type	TssF
T6SS ID (Type)	T6SS01254 (Type i1)
Strain	Vibrio parahaemolyticus strain 12-009A/1335
Replicon	chromosome
Sequence	Protein sequence (583 a.a.); Nucleotide sequence (1749 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	B5C29_RS17120
Coordinate (Strand)	26827..28575 (+)
NCBI ID	491623126
RefSeq	NZ_MYFF01000478
Uniprot ID	Q87PU6_VIBPA, A0A0L7YGB9_VIBPH
KEGG ID	vpa:VP1405
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	4.9e-157	5..579

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-582	MSNSKYFQDELTYLRESGSEFAKYHPKLTHFLSEGTFDPDVERLLEGFAFLTGRIREKIDDELPELTQSLMTLLWPHYMRSIPSLCISELKPHTGSVTEKTVVKRGAEMASEQVEGTQCLFRTCYDVNLYPITITNIEQTNSRTSSAIDVTLSTEHGLELSRIGLDTLRLHLHGEIHITRTVFLWLFRYLDYVELDVGGGYKHRLGPEYVKPVGHEEDEALLPYSKNSFAGYRLLQELFSLPDKFMFFDITGLEWLKGIPQRSTVKIKFHFKRALPSEVVLKDKHLRLHCTPAVNLFEKDGDPIRLEHRRNEYKVRPQSNTQEHYEVYSIEQVESWSKDERRRKPLIEFESFEHQINQRDKREFYKSKVGERVSGRGLERYISFHTHNGDIADLGTETVLMKLQCSNADLAERLSVGDITYATHKSPTYATFKNITKPTQSVSPQVNGELQWQLIANMSLNYLSLANIDVLKVLLSTYDFHSRVDRQAHRASIHRLDGIVSSEMKPIDRVFRGVSVRGNQFKLVTNSKFFVNEGDMFLMATVLNEFIRLYSSVNSFTELEVFDEATGEVYNWKSLIGQQTIL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9959	-	-

Protein sequence: 583 a.a.

						>T6CP015719 NZ_MYFF01000478:26827-28575 [Vibrio parahaemolyticus] [TssF]
MSNSKYFQDELTYLRESGSEFAKYHPKLTHFLSEGTFDPDVERLLEGFAFLTGRIREKIDDELPELTQSLMTLLWPHYMR
SIPSLCISELKPHTGSVTEKTVVKRGAEMASEQVEGTQCLFRTCYDVNLYPITITNIEQTNSRTSSAIDVTLSTEHGLEL
SRIGLDTLRLHLHGEIHITRTVFLWLFRYLDYVELDVGGGYKHRLGPEYVKPVGHEEDEALLPYSKNSFAGYRLLQELFS
LPDKFMFFDITGLEWLKGIPQRSTVKIKFHFKRALPSEVVLKDKHLRLHCTPAVNLFEKDGDPIRLEHRRNEYKVRPQSN
TQEHYEVYSIEQVESWSKDERRRKPLIEFESFEHQINQRDKREFYKSKVGERVSGRGLERYISFHTHNGDIADLGTETVL
MKLQCSNADLAERLSVGDITYATHKSPTYATFKNITKPTQSVSPQVNGELQWQLIANMSLNYLSLANIDVLKVLLSTYDF
HSRVDRQAHRASIHRLDGIVSSEMKPIDRVFRGVSVRGNQFKLVTNSKFFVNEGDMFLMATVLNEFIRLYSSVNSFTELE
VFDEATGEVYNWKSLIGQQTIL*					

Nucleotide sequence: 1749 bp

						>T6CP015719 NZ_MYFF01000478:26827-28575 [Vibrio parahaemolyticus] [TssF]
TTGAGTAATAGTAAATACTTTCAAGATGAACTCACATATTTGCGCGAATCGGGTAGTGAGTTTGCCAAATATCATCCGAA
GTTAACTCACTTTCTCTCTGAAGGAACATTCGACCCAGATGTAGAGCGATTGCTGGAAGGTTTCGCTTTTCTGACTGGAC
GTATTCGAGAGAAGATAGATGACGAACTGCCAGAACTGACGCAATCTCTGATGACTTTGCTGTGGCCGCATTACATGCGT
TCAATTCCATCTTTGTGCATCAGCGAGCTAAAACCACATACGGGAAGTGTTACTGAAAAAACGGTGGTAAAACGTGGCGC
AGAAATGGCCAGTGAGCAAGTGGAGGGCACGCAATGTCTTTTCCGCACTTGCTACGATGTGAATCTGTACCCAATCACGA
TCACCAACATTGAACAAACCAACAGCCGCACCAGTTCAGCGATTGATGTGACGTTGTCGACGGAGCATGGCCTAGAGCTG
TCTCGAATTGGTTTAGATACGTTGCGTTTGCACTTGCATGGTGAGATTCATATTACCCGCACTGTCTTCCTCTGGCTGTT
CCGTTACTTGGATTACGTGGAGTTGGATGTCGGTGGCGGCTATAAACATCGTTTAGGGCCAGAATATGTTAAGCCGGTAG
GGCATGAAGAAGACGAAGCGCTTCTGCCTTACAGCAAAAATTCATTCGCGGGTTATCGTTTGCTGCAAGAATTATTCTCT
TTGCCAGACAAGTTTATGTTCTTCGACATTACAGGTTTGGAGTGGCTAAAGGGTATTCCACAACGAAGCACAGTGAAAAT
CAAATTTCACTTTAAACGCGCATTGCCGTCAGAGGTTGTACTTAAAGATAAGCACCTAAGGTTGCACTGCACGCCGGCGG
TGAATTTGTTTGAAAAAGATGGCGACCCAATTCGTTTGGAGCACCGCCGTAACGAATACAAAGTCCGTCCTCAAAGTAAT
ACGCAAGAGCACTATGAAGTGTATTCCATTGAGCAAGTTGAGAGTTGGAGCAAAGACGAGCGTCGCCGTAAACCGTTAAT
TGAATTCGAATCGTTCGAGCATCAAATCAACCAACGAGACAAGCGAGAATTCTACAAGTCGAAGGTAGGAGAGCGTGTTA
GTGGAAGAGGGTTAGAGCGCTATATCTCGTTCCACACTCATAATGGTGATATTGCGGATTTAGGTACGGAAACCGTACTC
ATGAAACTGCAATGCAGTAACGCTGATTTGGCCGAACGTCTTTCGGTCGGCGACATTACTTACGCGACCCACAAATCGCC
AACGTATGCGACATTTAAAAACATCACCAAGCCAACACAATCGGTAAGCCCACAGGTAAACGGCGAGCTTCAATGGCAGT
TGATTGCCAATATGTCGCTCAATTATTTGTCTTTGGCAAACATTGATGTATTAAAAGTGCTGCTCTCGACCTATGACTTT
CATTCTCGTGTTGACAGACAAGCTCACCGAGCATCGATTCACCGACTCGATGGCATTGTATCGTCAGAGATGAAACCAAT
TGACCGTGTATTCCGTGGCGTGTCTGTGCGTGGTAACCAGTTCAAGCTAGTAACCAACTCTAAGTTCTTCGTAAATGAAG
GCGATATGTTCCTGATGGCAACGGTACTGAACGAATTCATCCGTTTGTATTCGAGTGTAAACTCCTTTACGGAGCTAGAA
GTATTTGATGAGGCAACCGGTGAAGTATACAACTGGAAAAGCTTAATCGGGCAGCAGACGATTCTATGA					