SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015711
Component protein type	TagH
T6SS ID (Type)	T6SS01253 (Type i5)
Strain	Vibrio parahaemolyticus strain 12-009A/1335
Replicon	chromosome
Sequence	Protein sequence (399 a.a.); Nucleotide sequence (1197 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	B5C29_RS20150
Coordinate (Strand)	29453..30649 (-)
NCBI ID	491606233
RefSeq	NZ_MYFF01000494
Uniprot ID	Q87HC2_VIBPA, A0A0L8RYZ1_VIBPH
KEGG ID	vpa:VPA1043
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
FHA	PF00498.28	1.2e-14	32..101

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-398	MKLVLTITSFHKFTPEIESEFVFESSDEHAKVTFGRSEQCDWTLPDPERVISGTHGELIKFGDKYLIKDLSTNGTFVNNAVTPIGQGNELALSHGDTVALGDYQIQISISDISEDRTEHQFTSNPEVKTPVGFEQHVESIPEMEDFGLSAAELMDESVPSDFQLDIGLVDDFLEIPSHETEVPQPKVASMQPDTNAKSQKPTVHRGELEAFIYGMGIDPNMVPSQNREQWFEQLGQSFSLMLIGLMETLHNRAVFKQTNRVNHTAFRKSENNPLKFSANLEDAIHNLYNRQTSSFLKPNAAIKEAFNDIENHEQALMQGVAGTVSSVMSLVEPNTIYSDALAKDNVINKVIPARKYAQSWKRFEGIHSQLTDELVNKESPFYLEDFAKHYELALKNKG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9679	-	-

Protein sequence: 399 a.a.

						>T6CP015711 NZ_MYFF01000494:c30649-29453 [Vibrio parahaemolyticus] [TagH]
MKLVLTITSFHKFTPEIESEFVFESSDEHAKVTFGRSEQCDWTLPDPERVISGTHGELIKFGDKYLIKDLSTNGTFVNNA
VTPIGQGNELALSHGDTVALGDYQIQISISDISEDRTEHQFTSNPEVKTPVGFEQHVESIPEMEDFGLSAAELMDESVPS
DFQLDIGLVDDFLEIPSHETEVPQPKVASMQPDTNAKSQKPTVHRGELEAFIYGMGIDPNMVPSQNREQWFEQLGQSFSL
MLIGLMETLHNRAVFKQTNRVNHTAFRKSENNPLKFSANLEDAIHNLYNRQTSSFLKPNAAIKEAFNDIENHEQALMQGV
AGTVSSVMSLVEPNTIYSDALAKDNVINKVIPARKYAQSWKRFEGIHSQLTDELVNKESPFYLEDFAKHYELALKNKG*					

Nucleotide sequence: 1197 bp

						>T6CP015711 NZ_MYFF01000494:c30649-29453 [Vibrio parahaemolyticus] [TagH]
ATGAAATTGGTATTGACCATAACGAGTTTCCACAAATTTACTCCGGAAATAGAGAGTGAGTTTGTGTTTGAATCAAGTGA
TGAGCATGCAAAGGTAACCTTCGGACGCTCAGAACAATGTGACTGGACGTTACCAGACCCTGAGAGAGTTATTTCTGGGA
CTCACGGCGAATTGATTAAATTTGGCGATAAATATCTAATTAAAGATTTATCAACAAATGGCACATTTGTTAATAATGCA
GTTACTCCAATCGGCCAAGGGAATGAGCTTGCTTTGTCCCATGGTGACACTGTTGCTTTAGGCGATTATCAAATACAAAT
TAGTATTAGTGATATCTCTGAAGATAGGACAGAGCATCAATTTACATCTAACCCAGAGGTTAAGACGCCAGTTGGATTCG
AACAGCACGTAGAATCGATTCCAGAGATGGAAGATTTTGGTTTAAGCGCTGCCGAGTTGATGGATGAAAGTGTCCCATCA
GATTTTCAGTTAGACATTGGTCTAGTAGATGATTTTCTAGAAATTCCGAGTCACGAAACTGAAGTGCCACAACCTAAAGT
TGCATCAATGCAGCCTGATACCAACGCTAAAAGCCAAAAACCTACAGTGCATCGAGGTGAACTGGAAGCCTTCATTTATG
GCATGGGGATCGACCCCAATATGGTACCAAGCCAAAATCGAGAGCAGTGGTTTGAACAATTAGGTCAGTCGTTTTCTCTC
ATGCTTATTGGGCTAATGGAGACTCTTCACAATCGGGCTGTATTTAAACAAACCAATCGCGTCAACCATACTGCATTTAG
AAAGTCTGAAAATAATCCACTTAAGTTTTCAGCAAATTTGGAAGATGCAATCCACAATTTGTATAACCGTCAAACGTCAA
GTTTTTTAAAGCCTAATGCCGCAATAAAAGAAGCATTTAACGATATTGAAAATCATGAGCAGGCTTTAATGCAAGGAGTA
GCGGGTACGGTATCGAGTGTCATGTCTCTCGTTGAACCTAATACTATTTATTCAGATGCATTGGCTAAAGACAATGTAAT
TAACAAGGTTATTCCAGCAAGAAAATATGCTCAGAGTTGGAAGCGCTTTGAAGGTATTCATAGCCAATTAACAGATGAAC
TTGTCAATAAAGAGTCTCCATTTTATTTAGAAGATTTTGCTAAGCACTACGAATTAGCGCTCAAGAATAAAGGTTAA					