SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015710
Component protein type	TssJ
T6SS ID (Type)	T6SS01253 (Type i5)
Strain	Vibrio parahaemolyticus strain 12-009A/1335
Replicon	chromosome
Sequence	Protein sequence (152 a.a.); Nucleotide sequence (456 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	B5C29_RS20145
Coordinate (Strand)	28988..29443 (-)
NCBI ID	491606235
RefSeq	NZ_MYFF01000494
Uniprot ID	Q87HC3_VIBPA, A0A2R9VI00_VIBPH
KEGG ID	vpa:VPA1042
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	5.7e-39	30..145

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	VKQIYK
Transmembrane helix	7-26	SFLLFIFLVLMGCSAANLVV
Outside	27-151	DPYADLEITANHNINPDSNGRPSPVVVYVFELTSNTLFESQDFFSIYEEHEKVLGPDLVNKYEISLTPGQKEIYQASMSPKTEYLGIVAAFRDIENSNWRQVIKVDKTGYNTYQISLEDLSLVVQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9804	pos: 18-19. LMG-CS.	VKQIYKSFLLFIFLVLMG

Protein sequence: 152 a.a.

						>T6CP015710 NZ_MYFF01000494:c29443-28988 [Vibrio parahaemolyticus] [TssJ]
VKQIYKSFLLFIFLVLMGCSAANLVVDPYADLEITANHNINPDSNGRPSPVVVYVFELTSNTLFESQDFFSIYEEHEKVL
GPDLVNKYEISLTPGQKEIYQASMSPKTEYLGIVAAFRDIENSNWRQVIKVDKTGYNTYQISLEDLSLVVQ*					

Nucleotide sequence: 456 bp

						>T6CP015710 NZ_MYFF01000494:c29443-28988 [Vibrio parahaemolyticus] [TssJ]
GTGAAACAAATTTATAAATCATTTTTGTTATTTATATTTTTAGTATTAATGGGATGCTCCGCCGCTAATTTAGTGGTCGA
CCCATATGCGGATTTAGAAATAACGGCAAATCATAATATCAACCCTGACTCGAATGGAAGACCATCACCAGTTGTTGTAT
ATGTATTTGAACTTACATCAAATACACTGTTTGAGAGCCAAGATTTTTTCTCTATTTATGAGGAGCATGAAAAAGTACTC
GGTCCAGACTTGGTGAATAAATATGAAATATCTTTGACACCAGGCCAAAAAGAAATCTACCAAGCTAGTATGTCTCCTAA
AACAGAATATTTGGGTATCGTAGCCGCGTTTCGCGATATTGAGAATTCAAATTGGCGACAAGTCATTAAAGTAGATAAGA
CGGGTTACAACACTTATCAAATCTCGTTAGAAGATCTGTCTTTGGTCGTACAATAA					