Detailed information of component protein


Summary

Component ID T6CP015708
Component protein type TssL
T6SS ID (Type) T6SS01253 experimental (Type i5)
Strain Vibrio parahaemolyticus strain 12-009A/1335
Replicon chromosome
Sequence Protein sequence (431 a.a.); Nucleotide sequence (1293 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) B5C29_RS20135
Coordinate (Strand) 26337..27629 (-)
NCBI ID 516016889
RefSeq NZ_MYFF01000494
Uniprot ID UPI000315C930
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1.1e-55 50..252
OmpA PF00691.22 2e-13 322..420
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-229 MEQTIVKPTPGGRAAVSKAQPQRSADSTVVISKSPELVNNDSVVAYGDNPLLAEANGLLSIIGQIRATATHSDPLFLKETLAQKLRDYENRLRQHDVDLETIDTARYCLCCSLDEAVLNTNWGSQSFWTHDSLLSSFYASSQGGEAFFKHLDSCLAHPESHLDLLELMYVCLSLGFIGQYRLEKNGLEAHRRLRKQVVSVLKSHGRGVQQELSNKVEQHILAGAQVSER
Transmembrane helix 230-252 APLWVVCSVTSALLVCIFMYFSY
Inside 253-430 ELNKASNQTFAQLVNLIQPTPAVSNPMVESKSAPIAERISMYLATEIGKDLVTVEALQDRVRISLKAQDLFESGSASVVAYIQPVISKVARTLEATQGKIIITGHTDDRPIFTSKYPSNWHLSLARATSLSEQLIANSALKGRVIPEGLGDARPLVENDSEENRAMNRRIEIDLIVGN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9408 - -


  Protein sequence: 431 a.a.    

>T6CP015708 NZ_MYFF01000494:c27629-26337 [Vibrio parahaemolyticus] [TssL]
MEQTIVKPTPGGRAAVSKAQPQRSADSTVVISKSPELVNNDSVVAYGDNPLLAEANGLLSIIGQIRATATHSDPLFLKET
LAQKLRDYENRLRQHDVDLETIDTARYCLCCSLDEAVLNTNWGSQSFWTHDSLLSSFYASSQGGEAFFKHLDSCLAHPES
HLDLLELMYVCLSLGFIGQYRLEKNGLEAHRRLRKQVVSVLKSHGRGVQQELSNKVEQHILAGAQVSERAPLWVVCSVTS
ALLVCIFMYFSYELNKASNQTFAQLVNLIQPTPAVSNPMVESKSAPIAERISMYLATEIGKDLVTVEALQDRVRISLKAQ
DLFESGSASVVAYIQPVISKVARTLEATQGKIIITGHTDDRPIFTSKYPSNWHLSLARATSLSEQLIANSALKGRVIPEG
LGDARPLVENDSEENRAMNRRIEIDLIVGN*

  Nucleotide sequence: 1293 bp    

>T6CP015708 NZ_MYFF01000494:c27629-26337 [Vibrio parahaemolyticus] [TssL]
ATGGAACAGACGATAGTAAAACCTACTCCTGGCGGAAGAGCGGCTGTATCTAAAGCTCAGCCTCAAAGATCAGCAGACAG
TACTGTTGTGATATCCAAAAGCCCTGAGTTGGTTAACAACGATTCAGTTGTTGCTTATGGAGATAATCCACTATTGGCAG
AAGCTAATGGTCTACTTTCGATTATTGGACAAATCAGAGCAACAGCTACTCATTCCGATCCTTTGTTTCTTAAAGAAACG
CTCGCGCAAAAACTTAGGGATTATGAGAATCGTCTGCGTCAACATGATGTTGACTTGGAAACCATTGATACCGCACGTTA
TTGTCTGTGCTGTTCTTTAGACGAAGCAGTATTGAATACCAATTGGGGGAGTCAATCGTTTTGGACACATGATTCTCTGT
TGAGTTCTTTTTACGCGAGCAGCCAAGGTGGAGAGGCTTTTTTTAAGCATCTAGATAGCTGTTTGGCTCATCCGGAATCC
CACCTAGATTTACTAGAGCTAATGTACGTATGCTTATCTTTGGGTTTTATAGGTCAGTATCGTTTAGAAAAAAATGGGCT
TGAAGCTCACCGTAGATTGAGAAAACAAGTTGTATCAGTGCTTAAGTCACATGGTCGCGGAGTACAGCAAGAGCTTAGTA
ACAAAGTAGAACAACATATATTGGCAGGTGCTCAGGTTTCGGAACGTGCTCCTCTATGGGTGGTTTGTTCGGTAACTTCC
GCACTGCTTGTTTGTATTTTTATGTACTTCAGCTACGAGTTGAACAAAGCTTCCAACCAAACTTTTGCTCAATTGGTAAA
CCTGATTCAACCAACCCCAGCAGTTAGTAATCCTATGGTTGAGTCAAAGTCAGCACCTATTGCCGAAAGAATATCTATGT
ATTTGGCAACGGAGATTGGCAAGGATCTTGTCACTGTGGAAGCGTTGCAAGATAGAGTTCGTATTTCTCTTAAAGCTCAA
GACCTATTTGAATCTGGTAGTGCGAGCGTTGTGGCTTACATACAGCCAGTTATTTCTAAAGTTGCACGTACCTTAGAAGC
GACACAAGGGAAGATCATAATTACCGGGCATACCGATGACCGTCCGATATTTACCAGCAAGTACCCATCCAATTGGCACT
TGTCTTTAGCTCGCGCAACTTCTCTTTCCGAACAGTTAATCGCCAACAGTGCACTTAAAGGCAGAGTGATTCCTGAAGGG
TTGGGTGATGCGAGACCTTTGGTGGAAAATGACAGTGAAGAGAACCGAGCAATGAATCGTCGAATTGAAATCGATCTTAT
TGTGGGCAACTAA