Detailed information of component protein


Summary

Component ID T6CP015707
Component protein type TssM
T6SS ID (Type) T6SS01253 experimental (Type i5)
Strain Vibrio parahaemolyticus strain 12-009A/1335
Replicon chromosome
Sequence Protein sequence (1173 a.a.); Nucleotide sequence (3519 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) B5C29_RS20130
Coordinate (Strand) 22803..26321 (-)
NCBI ID 686278489
RefSeq NZ_MYFF01000494
Uniprot ID UPI0004E6C985
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1.7e-86 193..450
IcmF-related PF06761.14 3.1e-76 504..802
IcmF_C PF06744.14 3.6e-18 1049..1149
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MSIKEIGRVFTQ
Transmembrane helix 13-32 RWFLGLVGVAACSIFIWVVG
Outside 33-46 PLITVAGYEPLKSD
Transmembrane helix 47-64 FQRLVTILVIVFAWALIN
Inside 65-445 LTKQHKQKVREDESIQTLLEVDSQSDKEAASEIDVMRDRIEQAIKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLAESLGADSIAGVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTISLASMMSQTRTERSLHARSIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFPREVDDERGVISLFNKEFHGMLERLDARMLRILETEDDLEKRTLIFEFPKQLRVLQANLDEFLSEIFAQNTFEEPALIRGVFLLSSVQEGIPVDRLMSESTNGLGLGRLPLATNVNSSHSYFVKNLFERVIFKEQLLGTVNRHYQKQSGWMRT
Transmembrane helix 446-468 GIYVGCVGVLVGASALWFLSYQW
Outside 469-1172 NSKLIVDTNSQVNHIEAMIGAESLDFESDVISAVDTLDKIMMLPLGKNSKYGHSDAVKKFGLYQGDKVSQAANNAYSDALSQHFATLLSESLVSEMEANKQHREYLYETLKTYLMLFNPEKYQQEEVIAWFNFYFERQYPGELNKELRERLLVHTKNLLENDEKGFSMDAAAISAAREVLTQMSLPERAYQRMKMQFAKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTVKGLMEENWVYGDDLKAHEIDHDSAIQGVQARYYQDYVNEWKTLIEDIQLKQAPSLALATEQSRVLSGVERPIESLLRAIQKEVGLSKVTLSENQKAATEVAAKVAKVKFSNTADKLDMYLPEENGFNVALPGKEVESHFTEILRLSEQDFDDIQQAMVNLRSYLSDLSSSGNNQKIAYKSILDGTVTQDVAASFARAKDLLPKPFNQWLGELSQESVKFAESGSKDHLNQLWVTNVVRPYQRTIAGRYPFEPNATKEVRLKDFQRFFGYGGTLDSFFQEYLEPFVDTSKSRWRLEKEIGVRPETLAVFQRAKRIRQSFFESDNSLRVEFGMKPVYLDQHITRFVLELGDQDLVYKHGPARSKELRWPSGQDQTRIVFTPPESKREIAHTYEGEWGIFKLLDQSLKARPESRNDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPQTL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.6736 - -


  Protein sequence: 1173 a.a.    

>T6CP015707 NZ_MYFF01000494:c26321-22803 [Vibrio parahaemolyticus] [TssM]
MSIKEIGRVFTQRWFLGLVGVAACSIFIWVVGPLITVAGYEPLKSDFQRLVTILVIVFAWALINLTKQHKQKVREDESIQ
TLLEVDSQSDKEAASEIDVMRDRIEQAIKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLAESLGADSIA
GVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTISLASMMSQTRTERSLHAR
SIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFPREVDDERGVISLFNKEFHGMLERLDAR
MLRILETEDDLEKRTLIFEFPKQLRVLQANLDEFLSEIFAQNTFEEPALIRGVFLLSSVQEGIPVDRLMSESTNGLGLGR
LPLATNVNSSHSYFVKNLFERVIFKEQLLGTVNRHYQKQSGWMRTGIYVGCVGVLVGASALWFLSYQWNSKLIVDTNSQV
NHIEAMIGAESLDFESDVISAVDTLDKIMMLPLGKNSKYGHSDAVKKFGLYQGDKVSQAANNAYSDALSQHFATLLSESL
VSEMEANKQHREYLYETLKTYLMLFNPEKYQQEEVIAWFNFYFERQYPGELNKELRERLLVHTKNLLENDEKGFSMDAAA
ISAAREVLTQMSLPERAYQRMKMQFAKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTV
KGLMEENWVYGDDLKAHEIDHDSAIQGVQARYYQDYVNEWKTLIEDIQLKQAPSLALATEQSRVLSGVERPIESLLRAIQ
KEVGLSKVTLSENQKAATEVAAKVAKVKFSNTADKLDMYLPEENGFNVALPGKEVESHFTEILRLSEQDFDDIQQAMVNL
RSYLSDLSSSGNNQKIAYKSILDGTVTQDVAASFARAKDLLPKPFNQWLGELSQESVKFAESGSKDHLNQLWVTNVVRPY
QRTIAGRYPFEPNATKEVRLKDFQRFFGYGGTLDSFFQEYLEPFVDTSKSRWRLEKEIGVRPETLAVFQRAKRIRQSFFE
SDNSLRVEFGMKPVYLDQHITRFVLELGDQDLVYKHGPARSKELRWPSGQDQTRIVFTPPESKREIAHTYEGEWGIFKLL
DQSLKARPESRNDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPQTL*

  Nucleotide sequence: 3519 bp    

>T6CP015707 NZ_MYFF01000494:c26321-22803 [Vibrio parahaemolyticus] [TssM]
ATGAGTATTAAAGAAATAGGCAGAGTATTTACCCAAAGATGGTTTCTTGGTTTAGTTGGTGTAGCCGCGTGCTCCATTTT
TATTTGGGTTGTGGGTCCTCTGATCACGGTTGCAGGTTACGAGCCGCTTAAAAGTGATTTCCAACGTTTAGTTACGATCT
TAGTGATTGTTTTTGCTTGGGCGCTTATCAACTTAACAAAGCAGCACAAACAGAAAGTTAGAGAAGACGAAAGTATTCAG
ACGTTGCTTGAAGTAGACAGTCAGTCAGACAAAGAAGCCGCCAGTGAAATTGACGTAATGCGCGATCGTATAGAGCAAGC
GATCAAAGTTGTGACAAAAACGCATAAAGGAAAGCGTAGTTTGTACGATTTACCTTGGTACGTTCTGATTGGTCCTCCTG
GAACAGGTAAAACCACGGTTCTAAAGCAATCTGGCTTGGAATTTCCGTTAGCTGAGAGCTTAGGGGCTGACTCTATAGCT
GGTGTAGGTGGAACACGTCACTGTGATTGGTGGTTTGCTAACAAAGCAGTACTTATTGATACAGCAGGACGTTACACCAC
GCAAGATAGTCAAGAAAAGGTTGACTCTAAAGCTTGGCACGGTTTCTTAGGGTTGCTTAAGAAATACCGCACTCAAAGGC
CTATCAATGGCGCTATCGTAACAATCAGCCTAGCAAGTATGATGTCTCAAACTCGTACAGAGCGTAGCTTACATGCCAGA
TCAATAAAATCTCGCCTTCAGGAACTCAAAAATCAGTTGGGTATGCAATTCCCAATCTATGTTCTGCTGACGAAAATGGA
TCTGGTTGCTGGCTTTAATGAATTTTTTGCTGATTTAAGTAAAGAAGAGCGTGAGGAACTTTTTGGATTTATGTTCCCAC
GAGAAGTTGATGACGAACGTGGTGTCATTTCTCTCTTTAATAAAGAGTTTCACGGCATGTTGGAGCGGCTCGATGCGCGC
ATGTTACGTATATTAGAAACTGAAGATGACTTAGAAAAGCGCACGCTAATATTCGAATTTCCAAAACAGCTGCGAGTTTT
ACAAGCAAACTTAGATGAGTTTTTGTCTGAGATATTTGCGCAGAACACGTTTGAAGAGCCAGCCCTTATTCGCGGTGTTT
TCTTGCTTAGCTCTGTACAAGAAGGTATTCCTGTTGATCGACTGATGAGTGAATCAACTAATGGATTGGGACTAGGGAGG
TTGCCTTTAGCGACAAACGTTAACAGTTCGCATAGCTATTTTGTTAAAAATCTTTTTGAGCGAGTCATCTTTAAAGAGCA
ATTACTAGGTACCGTTAACCGCCACTATCAAAAGCAATCTGGATGGATGCGTACGGGCATATACGTGGGTTGTGTTGGTG
TTCTTGTTGGTGCTTCTGCTCTTTGGTTCCTGAGTTATCAATGGAATTCAAAGTTAATTGTCGATACCAATAGCCAAGTA
AATCATATTGAAGCGATGATAGGTGCAGAGAGCCTTGATTTTGAATCGGACGTTATCAGTGCTGTAGACACGTTAGACAA
GATCATGATGTTGCCACTAGGGAAAAATAGTAAATATGGTCATTCTGATGCAGTGAAAAAGTTTGGTCTGTATCAAGGTG
ACAAAGTTTCTCAAGCAGCAAATAACGCGTATTCAGATGCTCTTTCTCAACACTTTGCTACTCTGCTGTCAGAGAGTTTA
GTCTCGGAAATGGAAGCGAATAAGCAGCATCGTGAATACTTATACGAGACGTTAAAGACGTATTTAATGTTGTTTAATCC
GGAAAAGTATCAACAAGAAGAGGTAATTGCTTGGTTCAACTTCTACTTTGAACGCCAGTATCCAGGAGAGTTAAACAAAG
AGCTACGTGAGCGTTTGCTAGTGCATACTAAAAACTTACTAGAGAACGACGAGAAAGGCTTCTCAATGGATGCGGCTGCT
ATTAGTGCAGCACGAGAAGTTCTAACGCAAATGTCACTTCCAGAGCGTGCATATCAGCGTATGAAGATGCAGTTTGCTAA
AAGCCACGTTCCGTCTTTCCGTTTAACAGACGTGTTAGGACCTAAAGGTTTAGAACAATTTGAGCGAGCAAGTGGAAAAC
CTCTATCGCAAGGTATTTCCGGTTTTTATACCTACAACGGTTTTCACAGCATTTTCCAAATTCAGATTAATCGCACCGTC
AAAGGATTGATGGAAGAAAACTGGGTGTATGGGGATGATCTTAAAGCTCATGAAATTGATCATGATAGTGCTATTCAGGG
GGTTCAAGCCCGCTATTATCAAGATTATGTCAATGAGTGGAAAACTCTGATAGAGGATATTCAGCTTAAACAAGCTCCAA
GTTTGGCTTTAGCAACAGAGCAATCAAGAGTTCTATCCGGTGTAGAAAGACCAATTGAATCACTTCTGCGTGCAATACAA
AAGGAAGTGGGGTTATCGAAAGTAACGTTATCGGAAAATCAAAAGGCAGCAACGGAAGTCGCAGCAAAGGTTGCAAAAGT
GAAGTTTTCTAATACGGCAGACAAACTCGATATGTATTTGCCTGAAGAGAATGGATTTAATGTTGCTCTACCAGGTAAAG
AAGTTGAATCCCATTTCACCGAAATCTTACGACTTAGTGAACAAGACTTTGATGATATTCAGCAAGCGATGGTGAATTTG
CGTTCGTACTTATCTGATTTGTCTAGCTCTGGCAACAATCAAAAAATTGCTTACAAAAGCATATTGGATGGAACGGTGAC
TCAGGATGTTGCTGCCTCCTTTGCTAGAGCAAAAGATCTCTTACCAAAACCGTTTAATCAATGGTTGGGAGAGTTGTCAC
AGGAATCGGTTAAGTTTGCAGAAAGCGGCTCTAAAGATCATCTAAATCAACTTTGGGTGACTAATGTAGTGCGTCCTTAT
CAGCGGACTATAGCAGGTAGATACCCATTTGAACCTAATGCAACGAAGGAAGTTCGTCTAAAGGACTTTCAACGATTCTT
CGGGTACGGTGGTACGTTGGACTCGTTCTTCCAAGAATATCTGGAGCCGTTTGTTGACACGTCTAAGAGTCGTTGGAGAC
TAGAAAAAGAAATCGGTGTAAGGCCCGAGACGCTTGCGGTTTTTCAAAGGGCAAAACGCATTAGACAATCGTTCTTTGAA
TCAGATAACTCGCTACGAGTCGAGTTTGGTATGAAACCCGTATATCTAGATCAACACATTACTCGATTTGTGCTAGAGCT
AGGTGATCAAGACTTGGTTTATAAGCACGGTCCTGCGAGATCGAAGGAACTTCGCTGGCCATCGGGTCAAGATCAGACGC
GTATTGTGTTTACACCGCCCGAATCAAAACGAGAAATTGCTCATACCTATGAAGGGGAATGGGGCATTTTCAAACTATTG
GACCAATCACTCAAGGCTCGACCAGAGTCCCGCAACGACAACATTGTCATGATCGATTTGAAGGGGAACAAGGTTCAGCT
TGAGTTGATTCCATCTAGTGCAATCAACCCATTTTGGTCTAATGAGATGGAGAGGTTCAGATGCCCGCAAACCTTGTAA