Detailed information of component protein
Summary
| Component ID | T6CP015704 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS01253  (Type i5) |
| Strain | Vibrio parahaemolyticus strain 12-009A/1335 |
| Replicon | chromosome |
| Sequence | Protein sequence (177 a.a.); Nucleotide sequence (531 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | B5C29_RS20110 |
| Coordinate (Strand) | 19663..20193 (-) |
| NCBI ID | WP_005463809.1 |
| RefSeq | NZ_MYFF01000494 |
| Uniprot ID | S5JDR9_VIBPH, Q87HD0_VIBPA, A0A072FX85_VIBPH |
| KEGG ID | vpa:VPA1035, vpf:M634_23095 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 1.6e-62 | 9..161 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-176 | MTSIHSKLSRVRKPRVHITYDVETEGTTLKKELPFVVGVMGDFAGQNTEALKPLKDRRFIQIDRDNFDDVLKKMSPSVKFKVANKLANDDSEFAVELSFKSMQDFEPAAIVNQVEPLRQLMETRNKLRDLMTKVDRSEELENILEEVLNNTDNLAKLAGELDLGKKAPSESTEGAE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9966 | - | - |
Protein sequence: 177 a.a.
>T6CP015704 NZ_MYFF01000494:c20193-19663 [Vibrio parahaemolyticus] [TssB]
MTSIHSKLSRVRKPRVHITYDVETEGTTLKKELPFVVGVMGDFAGQNTEALKPLKDRRFIQIDRDNFDDVLKKMSPSVKF
KVANKLANDDSEFAVELSFKSMQDFEPAAIVNQVEPLRQLMETRNKLRDLMTKVDRSEELENILEEVLNNTDNLAKLAGE
LDLGKKAPSESTEGAE*
MTSIHSKLSRVRKPRVHITYDVETEGTTLKKELPFVVGVMGDFAGQNTEALKPLKDRRFIQIDRDNFDDVLKKMSPSVKF
KVANKLANDDSEFAVELSFKSMQDFEPAAIVNQVEPLRQLMETRNKLRDLMTKVDRSEELENILEEVLNNTDNLAKLAGE
LDLGKKAPSESTEGAE*
Nucleotide sequence: 531 bp
>T6CP015704 NZ_MYFF01000494:c20193-19663 [Vibrio parahaemolyticus] [TssB]
ATGACGAGTATCCACTCGAAACTTTCACGAGTACGGAAGCCTCGTGTTCACATTACCTACGATGTTGAAACCGAAGGAAC
AACTTTGAAAAAAGAGCTTCCTTTTGTGGTGGGTGTTATGGGCGACTTTGCAGGCCAAAATACCGAAGCATTGAAACCAT
TAAAGGATCGCCGTTTCATTCAAATCGATCGTGATAACTTCGATGATGTACTTAAAAAGATGAGTCCATCGGTTAAGTTC
AAAGTCGCAAACAAGTTAGCAAATGACGATTCTGAATTTGCGGTTGAACTGAGCTTTAAATCGATGCAAGACTTTGAACC
AGCAGCGATTGTCAATCAAGTAGAACCATTGCGTCAGCTGATGGAAACTCGTAACAAACTGCGAGACTTGATGACTAAAG
TCGACCGTTCGGAAGAACTTGAAAACATCCTAGAAGAAGTTCTCAACAATACAGACAATTTGGCAAAACTAGCTGGTGAG
CTAGATCTTGGCAAAAAAGCGCCATCAGAAAGTACTGAGGGAGCAGAATAA
ATGACGAGTATCCACTCGAAACTTTCACGAGTACGGAAGCCTCGTGTTCACATTACCTACGATGTTGAAACCGAAGGAAC
AACTTTGAAAAAAGAGCTTCCTTTTGTGGTGGGTGTTATGGGCGACTTTGCAGGCCAAAATACCGAAGCATTGAAACCAT
TAAAGGATCGCCGTTTCATTCAAATCGATCGTGATAACTTCGATGATGTACTTAAAAAGATGAGTCCATCGGTTAAGTTC
AAAGTCGCAAACAAGTTAGCAAATGACGATTCTGAATTTGCGGTTGAACTGAGCTTTAAATCGATGCAAGACTTTGAACC
AGCAGCGATTGTCAATCAAGTAGAACCATTGCGTCAGCTGATGGAAACTCGTAACAAACTGCGAGACTTGATGACTAAAG
TCGACCGTTCGGAAGAACTTGAAAACATCCTAGAAGAAGTTCTCAACAATACAGACAATTTGGCAAAACTAGCTGGTGAG
CTAGATCTTGGCAAAAAAGCGCCATCAGAAAGTACTGAGGGAGCAGAATAA