SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015701
Component protein type	TssE
T6SS ID (Type)	T6SS01253 (Type i5)
Strain	Vibrio parahaemolyticus strain 12-009A/1335
Replicon	chromosome
Sequence	Protein sequence (155 a.a.); Nucleotide sequence (465 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	B5C29_RS20090
Coordinate (Strand)	15322..15786 (-)
NCBI ID	505126202
RefSeq	NZ_MYFF01000494
Uniprot ID	S5IXX4_VIBPH, A0A0F2HVX2_VIBPH
KEGG ID	vpf:M634_23075
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	5.2e-18	35..130

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-154	MALTVSLLDKLIDSDPTTREEKDFPVSQQHMLNGLLRDLESMLNSRIGWKNIHEDFLEVRYSILNYGLPDFSSMPYSSQDGQSQLCDIVYEAIREFEPRLRNPHVQISDEKNAIDRTLRLEISATCLIDNDIHELKFNSEVEPVNLGMKLSRAK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9951	-	-

Protein sequence: 155 a.a.

						>T6CP015701 NZ_MYFF01000494:c15786-15322 [Vibrio parahaemolyticus] [TssE]
MALTVSLLDKLIDSDPTTREEKDFPVSQQHMLNGLLRDLESMLNSRIGWKNIHEDFLEVRYSILNYGLPDFSSMPYSSQD
GQSQLCDIVYEAIREFEPRLRNPHVQISDEKNAIDRTLRLEISATCLIDNDIHELKFNSEVEPVNLGMKLSRAK*					

Nucleotide sequence: 465 bp

						>T6CP015701 NZ_MYFF01000494:c15786-15322 [Vibrio parahaemolyticus] [TssE]
ATGGCTTTAACGGTTTCACTGCTAGACAAGTTGATTGATAGCGACCCAACTACCAGAGAAGAAAAAGATTTTCCTGTCAG
TCAGCAACACATGCTCAATGGTTTGTTAAGAGACTTAGAGTCGATGCTTAATAGTCGTATTGGTTGGAAAAATATTCACG
AAGACTTTTTAGAAGTTAGATACTCAATTCTGAACTATGGGTTACCTGATTTTTCATCAATGCCTTATAGCAGCCAAGAT
GGACAAAGTCAGTTGTGTGACATCGTTTATGAAGCTATTCGGGAGTTTGAACCTCGGTTAAGAAATCCGCACGTTCAAAT
TTCTGATGAAAAAAATGCGATAGATCGAACCTTACGGTTAGAAATTAGCGCAACTTGTTTGATTGATAATGACATCCACG
AGTTGAAATTCAATTCGGAAGTCGAGCCAGTGAACTTAGGAATGAAGTTGAGTCGAGCGAAATGA					