SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015699
Component protein type	TssG
T6SS ID (Type)	T6SS01253 (Type i5)
Strain	Vibrio parahaemolyticus strain 12-009A/1335
Replicon	chromosome
Sequence	Protein sequence (322 a.a.); Nucleotide sequence (966 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	B5C29_RS20080
Coordinate (Strand)	12540..13505 (-)
NCBI ID	686278493
RefSeq	NZ_MYFF01000494
Uniprot ID	UPI0004E6AE12
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.9e-69	11..306

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-321	MSSEDFYRELKQRSLFEAIHLIEQELLKVESQIGTDALPKNEKLNLKVNASLGFENAQLVSTKPLGKDKLALETNLIGLTGEQGVLPQHYSELALHRLKEGDHAMVDFYDIFNHRLLSLYYRSWQLSQLTIQARAHAKNQRSPLTDCMSSLTGDGNDLALHYGGMYASPTRSKGALKSILECLSGCQIRIHEFQGQWMRLSKSEQTRLASKSMPEGKFAQLGAGASLGKKAWNINASTTIEFLPKESKQVKNLLPKTNTLKTIKRVAGDFIGKHKHVKWQLTTKHSLLPQVQISKQQGQLGVGSVLKKHERTEDRNITITV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9908	-	-

Protein sequence: 322 a.a.

						>T6CP015699 NZ_MYFF01000494:c13505-12540 [Vibrio parahaemolyticus] [TssG]
MSSEDFYRELKQRSLFEAIHLIEQELLKVESQIGTDALPKNEKLNLKVNASLGFENAQLVSTKPLGKDKLALETNLIGLT
GEQGVLPQHYSELALHRLKEGDHAMVDFYDIFNHRLLSLYYRSWQLSQLTIQARAHAKNQRSPLTDCMSSLTGDGNDLAL
HYGGMYASPTRSKGALKSILECLSGCQIRIHEFQGQWMRLSKSEQTRLASKSMPEGKFAQLGAGASLGKKAWNINASTTI
EFLPKESKQVKNLLPKTNTLKTIKRVAGDFIGKHKHVKWQLTTKHSLLPQVQISKQQGQLGVGSVLKKHERTEDRNITIT
V*					

Nucleotide sequence: 966 bp

						>T6CP015699 NZ_MYFF01000494:c13505-12540 [Vibrio parahaemolyticus] [TssG]
ATGAGCTCAGAGGACTTCTACCGCGAGCTAAAGCAACGTTCTTTGTTTGAAGCTATTCATCTTATTGAGCAAGAGCTTTT
GAAAGTTGAATCGCAAATTGGAACAGATGCATTACCAAAAAACGAGAAACTCAACTTAAAGGTCAATGCGTCTTTAGGCT
TTGAGAATGCGCAACTCGTTTCTACTAAACCATTAGGTAAAGATAAATTAGCGCTTGAGACAAATTTGATTGGCTTGACG
GGGGAGCAGGGTGTACTGCCTCAGCATTACAGTGAGCTTGCATTGCATCGACTGAAAGAAGGCGATCATGCGATGGTGGA
CTTCTATGACATTTTTAACCACCGGCTTTTATCCCTCTATTATCGAAGTTGGCAGTTGAGTCAGTTAACCATACAAGCGC
GTGCGCATGCAAAAAATCAGCGCTCCCCATTAACTGATTGCATGTCGTCATTGACTGGCGATGGGAATGATCTAGCACTG
CATTATGGCGGCATGTATGCATCTCCAACACGAAGTAAAGGTGCATTGAAGAGTATTTTGGAATGTTTGTCTGGTTGCCA
AATTCGTATTCACGAGTTTCAAGGTCAATGGATGCGACTGTCTAAATCTGAACAAACTCGTTTGGCATCGAAAAGTATGC
CCGAAGGCAAGTTTGCACAACTCGGTGCTGGTGCGTCACTAGGAAAAAAAGCCTGGAATATCAATGCAAGTACAACAATT
GAGTTTCTCCCAAAGGAAAGTAAGCAAGTCAAAAATCTATTACCCAAGACCAATACATTGAAAACGATAAAACGGGTAGC
AGGTGACTTCATTGGCAAACACAAGCACGTTAAGTGGCAACTTACGACTAAACATAGTCTATTGCCTCAAGTTCAAATAT
CTAAGCAACAAGGTCAGTTAGGTGTCGGCTCTGTACTGAAAAAACATGAACGTACGGAAGATCGCAACATAACAATTACG
GTTTAA					