SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015682
Component protein type	TssD
T6SS ID (Type)	T6SS01251 (Type i1)
Strain	Vibrio parahaemolyticus strain D4
Replicon	chromosome
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	CGI87_RS23505
Coordinate (Strand)	20436..20954 (-)
NCBI ID	491623110
RefSeq	NZ_NNJE01000075
Uniprot ID	Q79YY3_VIBPA, Q938W4_VIBPH
KEGG ID	vpa:VP1393
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	3.3e-30	6..155

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MPTPAYMSINGETQGHITKDTYSADSVGNTWQEAHVDEFLVQELDHVLTVPRDPQSGQPTGQRVHRPLVVTKVQDRSSPLLFNALVSGEKLPECLIRFYRTSVQGKQEHYYSIKLIDALLVDIQTRMNHCQDAATADRVTEEVLKFTYRAIEVTHENCGTAGNDDWRAPREA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9658	-	-

Protein sequence: 173 a.a.

						>T6CP015682 NZ_NNJE01000075:c20954-20436 [Vibrio parahaemolyticus] [TssD]
MPTPAYMSINGETQGHITKDTYSADSVGNTWQEAHVDEFLVQELDHVLTVPRDPQSGQPTGQRVHRPLVVTKVQDRSSPL
LFNALVSGEKLPECLIRFYRTSVQGKQEHYYSIKLIDALLVDIQTRMNHCQDAATADRVTEEVLKFTYRAIEVTHENCGT
AGNDDWRAPREA*					

Nucleotide sequence: 519 bp

						>T6CP015682 NZ_NNJE01000075:c20954-20436 [Vibrio parahaemolyticus] [TssD]
ATGCCAACTCCAGCATATATGTCTATCAACGGTGAAACTCAAGGTCACATTACTAAAGATACATACTCTGCAGATTCAGT
AGGTAACACATGGCAAGAAGCTCACGTTGATGAGTTCCTAGTACAAGAACTTGATCACGTGCTAACTGTTCCACGTGATC
CACAAAGTGGTCAACCTACTGGTCAACGTGTACACCGTCCACTTGTTGTGACTAAAGTTCAAGACCGTTCTTCTCCACTA
CTATTTAACGCATTAGTGTCTGGTGAAAAACTTCCTGAGTGTTTGATTCGTTTCTACCGTACTTCAGTTCAAGGCAAGCA
AGAGCACTACTACTCAATCAAGCTGATTGATGCGCTATTGGTAGATATCCAAACTCGTATGAACCACTGTCAAGATGCAG
CAACAGCTGATCGCGTTACAGAAGAAGTTCTTAAGTTTACTTACCGTGCAATCGAAGTAACTCACGAAAACTGTGGTACA
GCTGGTAACGACGACTGGCGTGCTCCACGCGAAGCATAA					