SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015676
Component protein type	TssF
T6SS ID (Type)	T6SS01251 (Type i1)
Strain	Vibrio parahaemolyticus strain D4
Replicon	chromosome
Sequence	Protein sequence (583 a.a.); Nucleotide sequence (1749 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	CGI87_RS23450
Coordinate (Strand)	6433..8181 (-)
NCBI ID	491623126
RefSeq	NZ_NNJE01000075
Uniprot ID	Q87PU6_VIBPA, A0A0L7YGB9_VIBPH
KEGG ID	vpa:VP1405
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	4.9e-157	5..579

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-582	MSNSKYFQDELTYLRESGSEFAKYHPKLTHFLSEGTFDPDVERLLEGFAFLTGRIREKIDDELPELTQSLMTLLWPHYMRSIPSLCISELKPHTGSVTEKTVVKRGAEMASEQVEGTQCLFRTCYDVNLYPITITNIEQTNSRTSSAIDVTLSTEHGLELSRIGLDTLRLHLHGEIHITRTVFLWLFRYLDYVELDVGGGYKHRLGPEYVKPVGHEEDEALLPYSKNSFAGYRLLQELFSLPDKFMFFDITGLEWLKGIPQRSTVKIKFHFKRALPSEVVLKDKHLRLHCTPAVNLFEKDGDPIRLEHRRNEYKVRPQSNTQEHYEVYSIEQVESWSKDERRRKPLIEFESFEHQINQRDKREFYKSKVGERVSGRGLERYISFHTHNGDIADLGTETVLMKLQCSNADLAERLSVGDITYATHKSPTYATFKNITKPTQSVSPQVNGELQWQLIANMSLNYLSLANIDVLKVLLSTYDFHSRVDRQAHRASIHRLDGIVSSEMKPIDRVFRGVSVRGNQFKLVTNSKFFVNEGDMFLMATVLNEFIRLYSSVNSFTELEVFDEATGEVYNWKSLIGQQTIL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9959	-	-

Protein sequence: 583 a.a.

						>T6CP015676 NZ_NNJE01000075:c8181-6433 [Vibrio parahaemolyticus] [TssF]
MSNSKYFQDELTYLRESGSEFAKYHPKLTHFLSEGTFDPDVERLLEGFAFLTGRIREKIDDELPELTQSLMTLLWPHYMR
SIPSLCISELKPHTGSVTEKTVVKRGAEMASEQVEGTQCLFRTCYDVNLYPITITNIEQTNSRTSSAIDVTLSTEHGLEL
SRIGLDTLRLHLHGEIHITRTVFLWLFRYLDYVELDVGGGYKHRLGPEYVKPVGHEEDEALLPYSKNSFAGYRLLQELFS
LPDKFMFFDITGLEWLKGIPQRSTVKIKFHFKRALPSEVVLKDKHLRLHCTPAVNLFEKDGDPIRLEHRRNEYKVRPQSN
TQEHYEVYSIEQVESWSKDERRRKPLIEFESFEHQINQRDKREFYKSKVGERVSGRGLERYISFHTHNGDIADLGTETVL
MKLQCSNADLAERLSVGDITYATHKSPTYATFKNITKPTQSVSPQVNGELQWQLIANMSLNYLSLANIDVLKVLLSTYDF
HSRVDRQAHRASIHRLDGIVSSEMKPIDRVFRGVSVRGNQFKLVTNSKFFVNEGDMFLMATVLNEFIRLYSSVNSFTELE
VFDEATGEVYNWKSLIGQQTIL*					

Nucleotide sequence: 1749 bp

						>T6CP015676 NZ_NNJE01000075:c8181-6433 [Vibrio parahaemolyticus] [TssF]
TTGAGTAATAGTAAATACTTTCAAGATGAACTCACATATTTGCGCGAATCGGGTAGTGAATTTGCCAAATATCATCCGAA
GTTAACTCACTTTCTCTCTGAAGGAACATTCGACCCAGATGTCGAGCGATTGCTGGAAGGTTTCGCTTTTCTGACTGGAC
GTATTCGAGAGAAGATCGATGACGAACTGCCAGAACTGACGCAATCTCTGATGACTTTGCTGTGGCCGCATTACATGCGT
TCAATTCCATCTTTGTGCATCAGTGAGCTAAAACCTCATACGGGAAGTGTTACTGAGAAAACGGTGGTAAAACGTGGCGC
AGAAATGGCCAGTGAGCAAGTAGAGGGTACGCAATGTCTTTTCCGCACTTGCTACGATGTGAATCTGTACCCAATCACGA
TCACCAACATTGAACAAACCAACAGCCGCACCAGTTCAGCGATTGATGTGACGTTGTCGACGGAGCATGGCCTAGAGCTG
TCTCGAATTGGTTTAGATACGTTGCGTTTACACTTGCATGGTGAGATTCATATTACCCGCACTGTCTTCCTCTGGCTGTT
CCGTTACTTGGATTACGTGGAGTTGGATGTCGGTGGCGGCTATAAACATCGTTTAGGGCCAGAATATGTTAAGCCGGTAG
GGCATGAAGAAGACGAAGCGCTTCTGCCTTACAGCAAAAATTCATTCGCGGGTTATCGTTTGCTGCAAGAATTATTCTCT
TTGCCAGACAAGTTTATGTTCTTCGACATTACAGGTTTGGAGTGGCTAAAGGGTATTCCACAACGAAGCACAGTGAAAAT
CAAATTTCACTTTAAACGCGCATTGCCGTCAGAGGTTGTACTTAAAGATAAGCACCTAAGGTTGCACTGCACGCCGGCGG
TGAATTTGTTTGAAAAAGATGGCGACCCAATTCGTTTGGAGCACCGTCGTAACGAATACAAAGTCCGTCCTCAAAGTAAT
ACGCAAGAGCACTATGAAGTGTATTCCATTGAGCAAGTTGAGAGTTGGAGCAAAGACGAGCGTCGCCGTAAACCGTTAAT
CGAATTCGAATCGTTCGAGCATCAAATCAACCAACGAGACAAGCGAGAATTCTACAAGTCGAAGGTAGGAGAGCGTGTTA
GTGGAAGAGGGTTAGAGCGCTATATCTCGTTCCACACTCATAATGGTGATATTGCGGATTTAGGTACGGAAACCGTACTC
ATGAAACTGCAATGCAGTAACGCTGATTTGGCCGAACGTCTTTCGGTCGGCGACATTACTTACGCGACTCACAAATCGCC
AACGTATGCGACATTTAAAAACATCACCAAGCCAACACAATCGGTAAGCCCACAGGTAAACGGCGAGCTTCAATGGCAGT
TGATTGCCAACATGTCGCTCAATTATTTGTCTTTGGCAAACATTGATGTATTAAAAGTGCTGCTCTCGACCTATGACTTT
CATTCTCGTGTTGACAGACAAGCTCACCGAGCATCGATTCACCGACTCGATGGCATTGTATCGTCAGAGATGAAACCAAT
TGACCGTGTATTCCGTGGCGTGTCTGTGCGTGGTAACCAGTTCAAACTAGTAACCAACTCTAAGTTCTTCGTAAATGAAG
GCGATATGTTCCTGATGGCAACGGTACTGAACGAATTCATCCGTTTGTATTCGAGTGTAAACTCCTTTACCGAGCTAGAA
GTATTTGATGAGGCAACCGGCGAAGTATACAACTGGAAAAGCTTAATTGGGCAGCAGACGATTCTATGA					