SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015675
Component protein type	TssG
T6SS ID (Type)	T6SS01251 (Type i1)
Strain	Vibrio parahaemolyticus strain D4
Replicon	chromosome
Sequence	Protein sequence (333 a.a.); Nucleotide sequence (999 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	CGI87_RS23445
Coordinate (Strand)	5438..6436 (-)
NCBI ID	505109571
RefSeq	NZ_NNJE01000075
Uniprot ID	UPI0002A55128
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.3e-85	10..317

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-332	MINTLSEKSNEFSFYQAVLLLEKHYQFEPNSNFVAVGENKYFHQERIEFSVSPNLCFPKSDMEFIVHMERSGQQYSRVETNFLGLHGSSSPLPASYTEKLTGREPDDNPVKDFFDFFHNRYTSLLYRVWKKYRYHVQYQSGATDAFSGRMLHLAGLSGVMQDCGVAELDRAKVLSYVNQLSTRTRSPKLISGIVSHYFSLPSVRIEEWVYRRVEIADSQRNKLNRANCILGQSFHLGQSIADLNGKFNLCIDDIDFETFKQFSYEGELHKTLVGLMRFILRDPMSWDLKLTVNLDSIPNNQLGNGEGNNLGQTFWLGNPTDEDVKIRVIGSI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9971	-	-

Protein sequence: 333 a.a.

						>T6CP015675 NZ_NNJE01000075:c6436-5438 [Vibrio parahaemolyticus] [TssG]
MINTLSEKSNEFSFYQAVLLLEKHYQFEPNSNFVAVGENKYFHQERIEFSVSPNLCFPKSDMEFIVHMERSGQQYSRVET
NFLGLHGSSSPLPASYTEKLTGREPDDNPVKDFFDFFHNRYTSLLYRVWKKYRYHVQYQSGATDAFSGRMLHLAGLSGVM
QDCGVAELDRAKVLSYVNQLSTRTRSPKLISGIVSHYFSLPSVRIEEWVYRRVEIADSQRNKLNRANCILGQSFHLGQSI
ADLNGKFNLCIDDIDFETFKQFSYEGELHKTLVGLMRFILRDPMSWDLKLTVNLDSIPNNQLGNGEGNNLGQTFWLGNPT
DEDVKIRVIGSI*					

Nucleotide sequence: 999 bp

						>T6CP015675 NZ_NNJE01000075:c6436-5438 [Vibrio parahaemolyticus] [TssG]
ATGATTAATACACTTTCTGAAAAATCGAATGAGTTTAGTTTTTATCAAGCGGTTCTTTTGCTCGAGAAGCACTACCAGTT
TGAACCAAACTCTAACTTTGTCGCCGTGGGAGAGAACAAATATTTTCACCAGGAGCGAATTGAGTTCAGCGTGTCGCCTA
ATCTATGCTTCCCTAAGAGTGATATGGAATTTATCGTTCATATGGAACGAAGTGGGCAGCAGTATTCACGAGTCGAAACG
AACTTCCTTGGTTTGCATGGTTCGAGCTCTCCATTGCCTGCCTCTTATACGGAGAAACTTACTGGGCGTGAACCTGATGA
CAACCCGGTAAAAGATTTCTTTGATTTCTTCCATAATCGCTACACCAGTTTGCTCTATCGTGTCTGGAAGAAGTACCGTT
ATCATGTGCAGTACCAGAGTGGTGCGACAGACGCTTTTTCTGGACGGATGCTGCACTTAGCTGGTTTATCAGGTGTTATG
CAAGATTGTGGTGTTGCAGAGTTAGACCGCGCAAAAGTCCTGTCTTACGTTAATCAACTATCGACACGTACACGTTCTCC
TAAGCTAATTTCAGGTATCGTTTCACATTATTTCTCACTTCCTAGCGTTCGCATCGAAGAATGGGTTTATCGCCGTGTGG
AGATTGCAGACAGTCAACGAAATAAACTCAATCGAGCCAATTGCATATTAGGTCAGAGCTTTCATTTAGGGCAGAGTATC
GCAGATTTAAATGGTAAGTTTAATCTTTGCATTGATGACATTGACTTCGAGACATTTAAGCAGTTTTCTTACGAAGGTGA
GTTGCATAAAACGTTGGTTGGTTTAATGCGATTCATCTTACGTGACCCGATGTCTTGGGATCTCAAACTGACAGTTAATC
TCGACAGCATTCCAAATAACCAACTTGGCAATGGAGAAGGGAACAACTTAGGGCAGACATTTTGGTTAGGCAATCCCACA
GATGAAGATGTGAAGATCAGAGTAATTGGTAGCATTTAG					