SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015673
Component protein type	TagH
T6SS ID (Type)	T6SS01250 (Type i5)
Strain	Vibrio parahaemolyticus strain D4
Replicon	chromosome
Sequence	Protein sequence (399 a.a.); Nucleotide sequence (1197 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	CGI87_RS17220
Coordinate (Strand)	19453..20649 (-)
NCBI ID	491606233
RefSeq	NZ_NNJE01000049
Uniprot ID	Q87HC2_VIBPA, A0A0L8RYZ1_VIBPH
KEGG ID	vpa:VPA1043
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
FHA	PF00498.28	1.2e-14	32..101

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-398	MKLVLTITSFHKFTPEIESEFVFESSDEHAKVTFGRSEQCDWTLPDPERVISGTHGELIKFGDKYLIKDLSTNGTFVNNAVTPIGQGNELALSHGDTVALGDYQIQISISDISEDRTEHQFTSNPEVKTPVGFEQHVESIPEMEDFGLSAAELMDESVPSDFQLDIGLVDDFLEIPSHETEVPQPKVASMQPDTNAKSQKPTVHRGELEAFIYGMGIDPNMVPSQNREQWFEQLGQSFSLMLIGLMETLHNRAVFKQTNRVNHTAFRKSENNPLKFSANLEDAIHNLYNRQTSSFLKPNAAIKEAFNDIENHEQALMQGVAGTVSSVMSLVEPNTIYSDALAKDNVINKVIPARKYAQSWKRFEGIHSQLTDELVNKESPFYLEDFAKHYELALKNKG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9679	-	-

Protein sequence: 399 a.a.

						>T6CP015673 NZ_NNJE01000049:c20649-19453 [Vibrio parahaemolyticus] [TagH]
MKLVLTITSFHKFTPEIESEFVFESSDEHAKVTFGRSEQCDWTLPDPERVISGTHGELIKFGDKYLIKDLSTNGTFVNNA
VTPIGQGNELALSHGDTVALGDYQIQISISDISEDRTEHQFTSNPEVKTPVGFEQHVESIPEMEDFGLSAAELMDESVPS
DFQLDIGLVDDFLEIPSHETEVPQPKVASMQPDTNAKSQKPTVHRGELEAFIYGMGIDPNMVPSQNREQWFEQLGQSFSL
MLIGLMETLHNRAVFKQTNRVNHTAFRKSENNPLKFSANLEDAIHNLYNRQTSSFLKPNAAIKEAFNDIENHEQALMQGV
AGTVSSVMSLVEPNTIYSDALAKDNVINKVIPARKYAQSWKRFEGIHSQLTDELVNKESPFYLEDFAKHYELALKNKG*					

Nucleotide sequence: 1197 bp

						>T6CP015673 NZ_NNJE01000049:c20649-19453 [Vibrio parahaemolyticus] [TagH]
ATGAAATTGGTATTGACCATAACGAGTTTCCACAAATTTACTCCGGAAATAGAGAGTGAGTTTGTGTTTGAATCAAGTGA
TGAGCATGCAAAGGTAACCTTCGGACGCTCAGAACAATGTGACTGGACGTTACCAGACCCTGAGAGAGTTATTTCTGGGA
CTCACGGCGAATTGATTAAATTTGGCGATAAATATCTAATTAAAGATTTATCAACAAATGGCACATTTGTTAATAATGCA
GTTACTCCAATCGGCCAAGGGAATGAGCTTGCTTTGTCCCATGGTGACACTGTTGCTTTAGGCGATTATCAAATACAAAT
TAGTATTAGTGATATCTCTGAAGATAGGACAGAGCATCAATTTACATCTAACCCAGAGGTTAAGACGCCAGTTGGATTCG
AACAGCACGTAGAATCGATTCCAGAGATGGAAGATTTTGGTTTAAGCGCTGCCGAGTTGATGGATGAAAGTGTCCCATCA
GATTTTCAGTTAGACATTGGTCTAGTAGATGATTTTCTAGAAATTCCGAGTCACGAAACTGAAGTGCCACAACCTAAAGT
TGCATCAATGCAGCCTGATACCAACGCTAAAAGTCAAAAACCTACAGTGCATCGAGGTGAACTGGAAGCCTTCATTTATG
GCATGGGGATCGACCCCAATATGGTACCAAGCCAAAATCGAGAGCAGTGGTTTGAACAATTAGGTCAGTCGTTTTCTCTC
ATGCTTATTGGGCTAATGGAGACTCTTCACAATCGGGCTGTATTTAAACAAACCAATCGCGTCAACCATACTGCATTTAG
AAAGTCTGAAAATAATCCACTTAAGTTTTCAGCAAATTTAGAAGATGCAATACACAATTTGTATAACCGTCAAACGTCAA
GTTTTTTAAAGCCTAATGCCGCAATAAAAGAAGCATTTAACGATATTGAAAATCATGAGCAGGCTTTAATGCAAGGAGTA
GCGGGTACGGTATCGAGTGTCATGTCTCTCGTTGAACCTAATACTATTTATTCAGATGCATTGGCTAAAGACAATGTAAT
TAACAAGGTTATTCCAGCAAGAAAATATGCTCAGAGTTGGAAGCGCTTTGAAGGTATTCATAGCCAATTAACAGATGAAC
TTGTCAATAAAGAGTCTCCATTTTATTTAGAAGATTTTGCTAAGCACTACGAATTAGCGCTCAAGAATAAAGGTTAA					