SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015672
Component protein type	TssJ
T6SS ID (Type)	T6SS01250 (Type i5)
Strain	Vibrio parahaemolyticus strain D4
Replicon	chromosome
Sequence	Protein sequence (152 a.a.); Nucleotide sequence (456 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	CGI87_RS17215
Coordinate (Strand)	18988..19443 (-)
NCBI ID	491606235
RefSeq	NZ_NNJE01000049
Uniprot ID	Q87HC3_VIBPA, A0A2R9VI00_VIBPH
KEGG ID	vpa:VPA1042
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	5.7e-39	30..145

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	VKQIYK
Transmembrane helix	7-26	SFLLFIFLVLMGCSAANLVV
Outside	27-151	DPYADLEITANHNINPDSNGRPSPVVVYVFELTSNTLFESQDFFSIYEEHEKVLGPDLVNKYEISLTPGQKEIYQASMSPKTEYLGIVAAFRDIENSNWRQVIKVDKTGYNTYQISLEDLSLVVQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9804	pos: 18-19. LMG-CS.	VKQIYKSFLLFIFLVLMG

Protein sequence: 152 a.a.

						>T6CP015672 NZ_NNJE01000049:c19443-18988 [Vibrio parahaemolyticus] [TssJ]
VKQIYKSFLLFIFLVLMGCSAANLVVDPYADLEITANHNINPDSNGRPSPVVVYVFELTSNTLFESQDFFSIYEEHEKVL
GPDLVNKYEISLTPGQKEIYQASMSPKTEYLGIVAAFRDIENSNWRQVIKVDKTGYNTYQISLEDLSLVVQ*					

Nucleotide sequence: 456 bp

						>T6CP015672 NZ_NNJE01000049:c19443-18988 [Vibrio parahaemolyticus] [TssJ]
GTGAAACAAATTTATAAATCATTTTTGCTTTTTATATTTTTAGTATTAATGGGATGCTCCGCCGCTAATTTAGTGGTCGA
CCCATATGCGGATTTAGAAATAACGGCAAATCATAATATCAACCCTGACTCGAATGGAAGACCGTCACCAGTTGTTGTAT
ATGTATTTGAACTTACATCAAATACACTGTTTGAGAGCCAAGATTTTTTCTCTATTTATGAGGAGCATGAAAAAGTACTC
GGTCCAGACTTGGTGAATAAATATGAAATATCTTTGACACCAGGCCAAAAAGAAATCTACCAAGCTAGTATGTCTCCTAA
AACAGAATATTTGGGTATCGTAGCCGCGTTTCGCGATATTGAGAATTCAAATTGGCGACAAGTCATTAAAGTAGATAAGA
CGGGTTACAACACTTATCAAATCTCGTTAGAAGATCTGTCTTTGGTCGTACAATAA					