Detailed information of component protein


Summary

Component ID T6CP015669
Component protein type TssM
T6SS ID (Type) T6SS01250 experimental (Type i5)
Strain Vibrio parahaemolyticus strain D4
Replicon chromosome
Sequence Protein sequence (1173 a.a.); Nucleotide sequence (3519 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) CGI87_RS17200
Coordinate (Strand) 12803..16321 (-)
NCBI ID 526016841
RefSeq NZ_NNJE01000049
Uniprot ID UPI000359196C
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 6.2e-86 193..450
IcmF-related PF06761.14 6.3e-76 504..802
IcmF_C PF06744.14 3.6e-18 1049..1149
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MSIKEIGRVFTQ
Transmembrane helix 13-32 RWFLGLVGVAACSIFIWVVG
Outside 33-46 PLITVAGYEPLKSD
Transmembrane helix 47-64 FQRLVTILVIVFAWALIN
Inside 65-445 LTKQHKQKVREDESIQTLLEVDSQSDKEAASEIDVMRDRIEQAIKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLAESLGADSIAGVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTVSLASVMSQTRTERSLHARSIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFPREVDDERGVISLFNKEFHGMLERLDARMLRILETEDDLEKRTLIFEFPKQLRVLQANLDEFLSEIFAQNTFEEPALIRGVFLLSSVQEGIPVDRLMSESTNGLGLGRLPLATNVNSSHSYFVKNLFERVIFKEQLLGTVNRHYQKQSGWMRT
Transmembrane helix 446-468 GIYVGCVGVLVGASALWFLSYQW
Outside 469-1172 NSKLIVDTNSQANHIEAMIGAESLDFESDVISAVDTLDKIMMLPLGKNSEYGHSDAVKKFGLYQGDKVSQAANNAYSDALSQHFATLLSESLVSEMEANKQHREYLYETLKTYLMLFNPEKYQQEEVIAWFNFYFERQYPGELNKELRERLLVHTKNLLENDEKGFSMDATAISAAREVLTQMSLPERAYQRMKMQFAKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTVKGLMEENWVYGDDLKAHEIDHDSAIQGVQARYYQDYVNEWKTLIEDVQLKQAPSLALATEQSRVLSGVERPIESLLRAIQKEVGLSKVTLSENQKAATEVAGKVAKVKFSNTADKLDMYLPEENGFNVALPGKEVESHFTEILRLSEQDFDDIQQAMVNLRSYLSDLSSSGNNQKIAYKSILDGTVTQDVAASFARAKDLLPKPFNQWLGELSQESVKFAERGSKDHLNQLWVTNVVRPYQRTIAGRYPFEPNATKEVRLKDFQRFFGYGGTLDSFFQEYLEPFVDTSKSRWRLEKEIGVRPETLAVFQRAKRIRQSFFESDNSLRVEFGMKPVYLDQHITRFVLELGDQDLVYKHGPARSKELRWPSGQDQTRIVFTPPESKREIAHTYEGEWGIFKLLDQSLKARPESRNDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPQTL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.6736 - -


  Protein sequence: 1173 a.a.    

>T6CP015669 NZ_NNJE01000049:c16321-12803 [Vibrio parahaemolyticus] [TssM]
MSIKEIGRVFTQRWFLGLVGVAACSIFIWVVGPLITVAGYEPLKSDFQRLVTILVIVFAWALINLTKQHKQKVREDESIQ
TLLEVDSQSDKEAASEIDVMRDRIEQAIKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLAESLGADSIA
GVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTVSLASVMSQTRTERSLHAR
SIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFPREVDDERGVISLFNKEFHGMLERLDAR
MLRILETEDDLEKRTLIFEFPKQLRVLQANLDEFLSEIFAQNTFEEPALIRGVFLLSSVQEGIPVDRLMSESTNGLGLGR
LPLATNVNSSHSYFVKNLFERVIFKEQLLGTVNRHYQKQSGWMRTGIYVGCVGVLVGASALWFLSYQWNSKLIVDTNSQA
NHIEAMIGAESLDFESDVISAVDTLDKIMMLPLGKNSEYGHSDAVKKFGLYQGDKVSQAANNAYSDALSQHFATLLSESL
VSEMEANKQHREYLYETLKTYLMLFNPEKYQQEEVIAWFNFYFERQYPGELNKELRERLLVHTKNLLENDEKGFSMDATA
ISAAREVLTQMSLPERAYQRMKMQFAKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTV
KGLMEENWVYGDDLKAHEIDHDSAIQGVQARYYQDYVNEWKTLIEDVQLKQAPSLALATEQSRVLSGVERPIESLLRAIQ
KEVGLSKVTLSENQKAATEVAGKVAKVKFSNTADKLDMYLPEENGFNVALPGKEVESHFTEILRLSEQDFDDIQQAMVNL
RSYLSDLSSSGNNQKIAYKSILDGTVTQDVAASFARAKDLLPKPFNQWLGELSQESVKFAERGSKDHLNQLWVTNVVRPY
QRTIAGRYPFEPNATKEVRLKDFQRFFGYGGTLDSFFQEYLEPFVDTSKSRWRLEKEIGVRPETLAVFQRAKRIRQSFFE
SDNSLRVEFGMKPVYLDQHITRFVLELGDQDLVYKHGPARSKELRWPSGQDQTRIVFTPPESKREIAHTYEGEWGIFKLL
DQSLKARPESRNDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPQTL*

  Nucleotide sequence: 3519 bp    

>T6CP015669 NZ_NNJE01000049:c16321-12803 [Vibrio parahaemolyticus] [TssM]
ATGAGTATTAAAGAAATAGGCAGAGTATTTACCCAAAGATGGTTTCTTGGTTTAGTTGGTGTAGCCGCGTGCTCCATTTT
TATTTGGGTTGTGGGTCCTCTGATCACGGTTGCAGGTTACGAGCCGCTTAAAAGTGATTTCCAACGTTTAGTTACGATCT
TAGTGATTGTTTTTGCTTGGGCGCTTATCAACTTAACAAAGCAGCACAAACAAAAAGTTAGAGAAGACGAAAGTATTCAG
ACGTTGCTTGAAGTAGACAGTCAGTCAGACAAAGAAGCCGCCAGTGAAATTGACGTAATGCGCGATCGTATAGAGCAAGC
GATCAAAGTTGTGACAAAAACGCATAAAGGAAAGCGTAGTTTGTACGATTTACCTTGGTACGTTCTGATTGGTCCTCCTG
GAACAGGTAAAACCACGGTTCTAAAGCAATCTGGCTTGGAATTTCCGTTAGCTGAGAGCTTAGGAGCTGACTCTATAGCT
GGTGTAGGTGGAACACGTCACTGTGATTGGTGGTTTGCTAATAAAGCAGTACTTATTGATACAGCAGGACGTTACACCAC
GCAAGATAGTCAAGAAAAGGTTGACTCTAAAGCTTGGCACGGTTTCTTGGGGTTGCTTAAGAAATACCGCACTCAAAGGC
CTATCAATGGCGCTATCGTTACAGTCAGCCTAGCAAGTGTGATGTCTCAAACTCGTACAGAACGTAGCTTACATGCCAGA
TCAATAAAATCTCGCCTTCAGGAACTAAAAAATCAGTTGGGTATGCAATTCCCAATCTATGTTCTGCTGACGAAAATGGA
TCTGGTTGCTGGCTTTAATGAATTTTTTGCTGATTTAAGTAAAGAAGAGCGTGAGGAACTTTTTGGATTTATGTTCCCAC
GAGAAGTTGATGACGAACGTGGTGTCATTTCTCTCTTTAATAAAGAGTTTCACGGCATGTTGGAGCGGCTCGATGCGCGC
ATGTTACGTATATTAGAAACTGAAGATGACTTAGAAAAGCGCACGCTAATATTCGAATTTCCAAAACAGCTGCGAGTTTT
ACAAGCAAACTTAGATGAGTTTTTGTCTGAGATATTTGCGCAGAACACGTTTGAAGAGCCAGCCCTTATTCGCGGTGTTT
TCTTGCTTAGCTCTGTACAAGAAGGTATTCCTGTTGATCGACTGATGAGTGAATCAACTAATGGATTGGGACTAGGGAGG
TTGCCTTTAGCGACAAACGTTAACAGTTCGCATAGCTATTTTGTTAAGAATCTTTTTGAGCGAGTCATCTTTAAAGAGCA
ATTACTAGGTACCGTTAACCGCCACTATCAAAAGCAATCTGGATGGATGCGTACGGGCATATACGTGGGTTGTGTTGGTG
TTCTTGTTGGTGCTTCAGCTCTTTGGTTCCTGAGTTATCAATGGAATTCAAAGTTAATTGTCGATACCAATAGCCAAGCA
AATCATATTGAAGCGATGATAGGTGCAGAGAGCCTTGATTTTGAATCGGACGTTATCAGTGCTGTAGACACGTTAGACAA
GATCATGATGTTGCCACTAGGGAAAAATAGTGAATATGGTCATTCTGATGCAGTGAAAAAGTTTGGTCTGTATCAAGGTG
ACAAAGTTTCTCAAGCAGCAAATAACGCGTATTCAGATGCTCTTTCTCAACACTTTGCTACTCTGCTGTCAGAGAGTTTG
GTCTCGGAAATGGAAGCGAATAAGCAGCATCGTGAATACTTATACGAGACGTTAAAGACGTATTTAATGTTGTTTAATCC
GGAAAAGTATCAACAAGAAGAGGTAATTGCTTGGTTCAACTTCTACTTTGAACGTCAGTATCCAGGAGAGTTAAACAAAG
AGCTACGTGAGCGTTTGCTAGTGCATACTAAAAACTTACTAGAGAACGACGAGAAAGGCTTCTCAATGGATGCGACTGCT
ATTAGTGCAGCACGAGAAGTTCTAACGCAAATGTCACTTCCAGAGCGTGCATATCAGCGAATGAAGATGCAGTTTGCTAA
AAGTCACGTTCCGTCTTTCCGTTTAACAGATGTGTTAGGACCTAAAGGTTTAGAACAATTTGAGCGAGCAAGTGGAAAAC
CTCTATCGCAAGGTATTTCCGGTTTTTATACCTACAACGGTTTTCACAGCATTTTCCAAATTCAGATTAATCGCACCGTC
AAAGGATTGATGGAAGAAAACTGGGTGTATGGGGATGATCTTAAAGCTCATGAAATTGATCATGATAGTGCTATTCAGGG
GGTTCAAGCCCGCTATTATCAAGATTATGTCAATGAGTGGAAAACTCTGATAGAGGATGTTCAGCTTAAACAAGCTCCGA
GTTTGGCTTTAGCAACAGAGCAATCAAGAGTTTTATCCGGTGTAGAAAGACCAATTGAATCACTTCTGCGTGCAATACAA
AAGGAAGTGGGGTTATCGAAAGTAACGTTATCGGAAAATCAAAAGGCAGCAACGGAAGTCGCAGGAAAGGTTGCAAAAGT
GAAGTTTTCTAATACGGCCGACAAACTCGATATGTATTTGCCTGAAGAGAATGGATTTAATGTTGCTCTACCAGGTAAAG
AAGTTGAATCCCATTTCACCGAAATCTTACGACTTAGTGAACAAGACTTTGATGATATTCAGCAAGCGATGGTGAATTTG
CGTTCGTACTTATCTGATTTGTCTAGCTCTGGCAACAATCAAAAAATTGCTTACAAAAGCATATTGGATGGAACGGTGAC
TCAGGATGTTGCTGCCTCCTTTGCTAGAGCAAAAGATCTCTTACCAAAACCGTTTAATCAATGGTTGGGAGAGTTGTCAC
AGGAATCGGTTAAGTTTGCAGAAAGAGGCTCTAAAGATCATCTAAATCAGCTTTGGGTGACTAATGTAGTGCGTCCTTAT
CAGCGGACTATAGCAGGTAGATACCCATTTGAACCTAATGCAACGAAGGAAGTTCGTCTAAAGGACTTTCAACGATTCTT
CGGGTACGGTGGTACGTTGGACTCGTTCTTCCAAGAATATCTGGAGCCGTTTGTTGATACGTCTAAGAGTCGTTGGAGAC
TAGAAAAAGAAATCGGTGTAAGGCCCGAGACGCTTGCAGTTTTTCAAAGGGCAAAACGCATTAGACAATCGTTCTTTGAA
TCAGATAACTCGCTACGAGTCGAGTTTGGTATGAAACCCGTATATCTAGATCAACACATTACTCGATTTGTGCTAGAGCT
AGGTGATCAAGACTTGGTTTATAAGCACGGTCCTGCGAGATCGAAGGAACTTCGCTGGCCATCGGGTCAAGATCAGACGC
GTATTGTGTTTACACCGCCCGAATCAAAACGAGAAATTGCTCATACCTATGAAGGGGAATGGGGCATTTTCAAACTATTG
GACCAATCACTCAAGGCTCGACCAGAGTCCCGCAACGACAACATTGTCATGATCGATTTGAAGGGGAACAAGGTTCAGCT
TGAGTTGATTCCATCTAGTGCAATCAACCCATTTTGGTCTAATGAGATGGAGAGGTTCAGATGTCCGCAAACCTTGTAA