SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015667
Component protein type	TssA
T6SS ID (Type)	T6SS01250 (Type i5)
Strain	Vibrio parahaemolyticus strain D4
Replicon	chromosome
Sequence	Protein sequence (374 a.a.); Nucleotide sequence (1122 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	CGI87_RS17185
Coordinate (Strand)	10209..11330 (-)
NCBI ID	491622789
RefSeq	NZ_NNJE01000049
Uniprot ID	Q87HC9_VIBPA, A0A0L8UFB6_VIBPH
KEGG ID	vpa:VPA1036
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	1.1e-31	13..142

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-373	MSNTMFDWYEQVLAPISEDNPTGIDPREDVSPQSAYYRLKDQRMVARNAERNAIIEEESIHTHNNLWRVFVEEVPEVLATQSKDFEFVAWLIEALTRLYGFRGMGVGYKIASAFVESYWDELHPMPDEDGIETRISAITGLNGIDSEGTLIFPLASIPLTDMGVEQAYAYWEYQQAIELERLDEDKRRARVDQGAIELSRITDTVKSTSDEFYQSLIADLEFATGAFNEFSQRLDDAVGDVTPSSYISKRIESIHAALKHLLGNRFKDKAEVSISDENGDEELEEASQQAVTHNSSALLVTNMQSREQAIQQLQHVADFFRESEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAKRSYFRLVGIAEQNEN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9890	-	-

Protein sequence: 374 a.a.

						>T6CP015667 NZ_NNJE01000049:c11330-10209 [Vibrio parahaemolyticus] [TssA]
MSNTMFDWYEQVLAPISEDNPTGIDPREDVSPQSAYYRLKDQRMVARNAERNAIIEEESIHTHNNLWRVFVEEVPEVLAT
QSKDFEFVAWLIEALTRLYGFRGMGVGYKIASAFVESYWDELHPMPDEDGIETRISAITGLNGIDSEGTLIFPLASIPLT
DMGVEQAYAYWEYQQAIELERLDEDKRRARVDQGAIELSRITDTVKSTSDEFYQSLIADLEFATGAFNEFSQRLDDAVGD
VTPSSYISKRIESIHAALKHLLGNRFKDKAEVSISDENGDEELEEASQQAVTHNSSALLVTNMQSREQAIQQLQHVADFF
RESEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAKRSYFRLVGIAEQNEN*					

Nucleotide sequence: 1122 bp

						>T6CP015667 NZ_NNJE01000049:c11330-10209 [Vibrio parahaemolyticus] [TssA]
ATGAGTAACACGATGTTTGATTGGTATGAGCAAGTCCTTGCACCAATTTCGGAAGACAATCCGACTGGCATTGATCCAAG
AGAGGATGTCTCTCCTCAGTCTGCTTATTATCGACTTAAAGATCAGCGAATGGTGGCAAGAAACGCAGAGCGAAACGCCA
TCATCGAAGAAGAGAGTATTCATACACATAACAACTTATGGCGCGTTTTTGTTGAAGAAGTACCAGAAGTTCTCGCTACA
CAATCAAAAGATTTTGAGTTTGTTGCCTGGCTAATAGAAGCATTAACTCGGCTTTATGGTTTTAGAGGCATGGGCGTAGG
CTACAAAATTGCTAGCGCGTTTGTTGAGTCATATTGGGATGAGCTGCACCCAATGCCTGATGAAGACGGCATAGAAACGC
GGATTTCTGCCATCACCGGACTTAATGGTATTGATAGTGAGGGAACGCTGATCTTTCCTCTTGCCTCGATTCCATTGACG
GATATGGGTGTAGAGCAAGCTTATGCATATTGGGAATACCAACAAGCTATTGAACTCGAGCGCTTGGACGAAGACAAGCG
AAGAGCACGAGTAGATCAAGGCGCAATTGAATTGTCACGTATCACTGACACTGTGAAATCAACTTCTGATGAGTTTTACC
AGTCTCTGATTGCAGATCTCGAGTTCGCTACAGGTGCTTTTAATGAGTTCAGTCAGCGACTTGATGACGCTGTAGGGGAT
GTAACGCCTAGCTCATATATTTCAAAAAGAATTGAATCAATACACGCGGCACTTAAGCACTTGTTGGGTAATCGTTTTAA
GGATAAGGCTGAGGTCTCCATTTCTGACGAAAATGGTGATGAAGAGTTAGAAGAAGCAAGTCAACAAGCAGTTACTCACA
ACTCATCTGCGTTATTAGTTACAAACATGCAATCTCGAGAACAAGCTATTCAACAGTTGCAACATGTGGCGGACTTTTTT
AGAGAAAGCGAGCCTCACTCACCAGTTTCTTACTCCATCGAACAAATTATTCGTTGGTGTGGAATGCCTCTGCCTGAATT
ACTGGCGGAACTAATCTCCGACGGAGACGCAAAACGAAGCTACTTCCGTTTGGTTGGCATAGCAGAACAAAATGAAAATT
AA					