SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015663
Component protein type	TssE
T6SS ID (Type)	T6SS01250 (Type i5)
Strain	Vibrio parahaemolyticus strain D4
Replicon	chromosome
Sequence	Protein sequence (155 a.a.); Nucleotide sequence (465 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	CGI87_RS17160
Coordinate (Strand)	5322..5786 (-)
NCBI ID	505126202
RefSeq	NZ_NNJE01000049
Uniprot ID	S5IXX4_VIBPH, A0A0F2HVX2_VIBPH
KEGG ID	vpf:M634_23075
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	5.2e-18	35..130

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-154	MALTVSLLDKLIDSDPTTREEKDFPVSQQHMLNGLLRDLESMLNSRIGWKNIHEDFLEVRYSILNYGLPDFSSMPYSSQDGQSQLCDIVYEAIREFEPRLRNPHVQISDEKNAIDRTLRLEISATCLIDNDIHELKFNSEVEPVNLGMKLSRAK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9951	-	-

Protein sequence: 155 a.a.

						>T6CP015663 NZ_NNJE01000049:c5786-5322 [Vibrio parahaemolyticus] [TssE]
MALTVSLLDKLIDSDPTTREEKDFPVSQQHMLNGLLRDLESMLNSRIGWKNIHEDFLEVRYSILNYGLPDFSSMPYSSQD
GQSQLCDIVYEAIREFEPRLRNPHVQISDEKNAIDRTLRLEISATCLIDNDIHELKFNSEVEPVNLGMKLSRAK*					

Nucleotide sequence: 465 bp

						>T6CP015663 NZ_NNJE01000049:c5786-5322 [Vibrio parahaemolyticus] [TssE]
ATGGCTTTAACGGTTTCACTGCTAGACAAGTTGATTGATAGCGACCCAACTACCAGAGAAGAAAAAGATTTTCCTGTCAG
TCAGCAACACATGCTCAATGGTTTGTTAAGAGACTTAGAGTCGATGCTTAATAGTCGTATTGGTTGGAAAAATATTCACG
AAGACTTTTTAGAAGTTAGATACTCAATTCTGAACTATGGGTTACCTGATTTTTCTTCAATGCCTTATAGCAGCCAAGAT
GGACAAAGTCAGTTGTGTGACATCGTTTATGAAGCTATTCGGGAGTTTGAACCTCGGTTAAGAAATCCGCACGTTCAAAT
TTCTGATGAAAAAAATGCGATAGATCGAACCTTACGGTTAGAAATTAGCGCAACTTGTTTGATTGATAATGATATCCACG
AGTTGAAATTCAATTCGGAAGTCGAGCCTGTGAACTTAGGAATGAAGTTGAGTCGAGCGAAATGA					