SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015661
Component protein type	TssG
T6SS ID (Type)	T6SS01250 (Type i5)
Strain	Vibrio parahaemolyticus strain D4
Replicon	chromosome
Sequence	Protein sequence (322 a.a.); Nucleotide sequence (966 bp)
Reference	[1] Li P, Kinch LN, Ray A, et al. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: e00737-17. doi: 10.1128/AEM.00737-17. Print 2017 Jul 1. PMID: 28432099

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	CGI87_RS17150
Coordinate (Strand)	2540..3505 (-)
NCBI ID	526016834
RefSeq	NZ_NNJE01000049
Uniprot ID	UPI00035900B5
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	2.1e-70	11..306

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-321	MSSEDFYRELKQRSLFEAIHLIEQELLKVESQIGTDALPKNEKLNLKVNASLGFENAQLVSTKPLGKDKLALETNLIGLTGEQGVLPQHYSELALHRLKEGDHAMVDFYDIFNHRLLSLYYRSWQLSQLTIQARAHAKNQRSPLTDCMSSLTGDGNDLALHYGGMYASPTRSKGALKSILECLSGCQIRIHEFQGQWMRLSKSEQTRLASKSMPEGQFAQLGAGASLGKRAWNINASTTIEFLPKERKQVKNLLPKTNTLKTIKQVAGDFIGKHKHVKWQLTTKHSLLPQVQISKQQGQLGVGSVLKKHERTEDRNITITV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9908	-	-

Protein sequence: 322 a.a.

						>T6CP015661 NZ_NNJE01000049:c3505-2540 [Vibrio parahaemolyticus] [TssG]
MSSEDFYRELKQRSLFEAIHLIEQELLKVESQIGTDALPKNEKLNLKVNASLGFENAQLVSTKPLGKDKLALETNLIGLT
GEQGVLPQHYSELALHRLKEGDHAMVDFYDIFNHRLLSLYYRSWQLSQLTIQARAHAKNQRSPLTDCMSSLTGDGNDLAL
HYGGMYASPTRSKGALKSILECLSGCQIRIHEFQGQWMRLSKSEQTRLASKSMPEGQFAQLGAGASLGKRAWNINASTTI
EFLPKERKQVKNLLPKTNTLKTIKQVAGDFIGKHKHVKWQLTTKHSLLPQVQISKQQGQLGVGSVLKKHERTEDRNITIT
V*					

Nucleotide sequence: 966 bp

						>T6CP015661 NZ_NNJE01000049:c3505-2540 [Vibrio parahaemolyticus] [TssG]
ATGAGCTCAGAGGACTTCTACCGCGAGCTAAAGCAACGTTCTTTGTTTGAAGCTATTCATCTTATTGAGCAAGAGCTTTT
GAAAGTTGAATCGCAAATTGGAACAGATGCATTACCAAAAAACGAGAAACTTAACTTAAAGGTCAATGCGTCTTTAGGGT
TTGAGAATGCGCAACTCGTTTCTACTAAACCATTAGGTAAAGATAAATTAGCGCTTGAGACAAATTTGATTGGCTTGACG
GGGGAGCAGGGTGTACTGCCTCAGCATTACAGTGAGCTTGCATTACATCGACTGAAAGAAGGCGATCATGCGATGGTGGA
CTTCTATGACATTTTTAACCATCGGCTTTTATCCCTCTATTATCGAAGTTGGCAATTGAGTCAGTTAACCATACAAGCGC
GTGCGCATGCAAAAAATCAGCGCTCCCCATTGACTGATTGCATGTCGTCATTGACTGGCGATGGGAATGATCTTGCACTG
CATTATGGCGGCATGTATGCATCTCCAACACGAAGTAAAGGTGCATTGAAGAGCATTTTGGAATGTTTGTCTGGTTGCCA
AATTCGTATTCACGAGTTTCAAGGTCAATGGATGCGACTGTCTAAATCTGAACAAACTCGTTTGGCATCGAAAAGTATGC
CCGAAGGCCAGTTTGCACAACTCGGTGCTGGTGCGTCACTAGGGAAAAGAGCCTGGAATATCAATGCAAGTACAACAATT
GAGTTTCTCCCAAAGGAAAGAAAGCAAGTCAAAAATCTGTTACCCAAGACCAATACATTGAAAACGATAAAACAGGTAGC
AGGTGACTTCATTGGCAAACACAAGCACGTTAAATGGCAACTCACGACTAAACATAGTCTATTGCCTCAAGTTCAAATAT
CTAAGCAACAAGGTCAGTTAGGTGTCGGCTCTGTACTGAAAAAACATGAACGTACGGAAGATCGCAACATAACAATTACG
GTTTAA					