SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015651
Component protein type	TssF
T6SS ID (Type)	T6SS01247 (Type i1)
Strain	Vibrio parahaemolyticus strain T9109
Replicon	chromosome
Sequence	Protein sequence (583 a.a.); Nucleotide sequence (1749 bp)
Reference	[1] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PO79_RS04595
Coordinate (Strand)	219887..221635 (-)
NCBI ID	491623126
RefSeq	NZ_JTGR01000038
Uniprot ID	Q87PU6_VIBPA, A0A0L7YGB9_VIBPH
KEGG ID	vpa:VP1405
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	4.9e-157	5..579

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-582	MSNSKYFQDELTYLRESGSEFAKYHPKLTHFLSEGTFDPDVERLLEGFAFLTGRIREKIDDELPELTQSLMTLLWPHYMRSIPSLCISELKPHTGSVTEKTVVKRGAEMASEQVEGTQCLFRTCYDVNLYPITITNIEQTNSRTSSAIDVTLSTEHGLELSRIGLDTLRLHLHGEIHITRTVFLWLFRYLDYVELDVGGGYKHRLGPEYVKPVGHEEDEALLPYSKNSFAGYRLLQELFSLPDKFMFFDITGLEWLKGIPQRSTVKIKFHFKRALPSEVVLKDKHLRLHCTPAVNLFEKDGDPIRLEHRRNEYKVRPQSNTQEHYEVYSIEQVESWSKDERRRKPLIEFESFEHQINQRDKREFYKSKVGERVSGRGLERYISFHTHNGDIADLGTETVLMKLQCSNADLAERLSVGDITYATHKSPTYATFKNITKPTQSVSPQVNGELQWQLIANMSLNYLSLANIDVLKVLLSTYDFHSRVDRQAHRASIHRLDGIVSSEMKPIDRVFRGVSVRGNQFKLVTNSKFFVNEGDMFLMATVLNEFIRLYSSVNSFTELEVFDEATGEVYNWKSLIGQQTIL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9959	-	-

Protein sequence: 583 a.a.

						>T6CP015651 NZ_JTGR01000038:c221635-219887 [Vibrio parahaemolyticus] [TssF]
MSNSKYFQDELTYLRESGSEFAKYHPKLTHFLSEGTFDPDVERLLEGFAFLTGRIREKIDDELPELTQSLMTLLWPHYMR
SIPSLCISELKPHTGSVTEKTVVKRGAEMASEQVEGTQCLFRTCYDVNLYPITITNIEQTNSRTSSAIDVTLSTEHGLEL
SRIGLDTLRLHLHGEIHITRTVFLWLFRYLDYVELDVGGGYKHRLGPEYVKPVGHEEDEALLPYSKNSFAGYRLLQELFS
LPDKFMFFDITGLEWLKGIPQRSTVKIKFHFKRALPSEVVLKDKHLRLHCTPAVNLFEKDGDPIRLEHRRNEYKVRPQSN
TQEHYEVYSIEQVESWSKDERRRKPLIEFESFEHQINQRDKREFYKSKVGERVSGRGLERYISFHTHNGDIADLGTETVL
MKLQCSNADLAERLSVGDITYATHKSPTYATFKNITKPTQSVSPQVNGELQWQLIANMSLNYLSLANIDVLKVLLSTYDF
HSRVDRQAHRASIHRLDGIVSSEMKPIDRVFRGVSVRGNQFKLVTNSKFFVNEGDMFLMATVLNEFIRLYSSVNSFTELE
VFDEATGEVYNWKSLIGQQTIL*					

Nucleotide sequence: 1749 bp

						>T6CP015651 NZ_JTGR01000038:c221635-219887 [Vibrio parahaemolyticus] [TssF]
TTGAGTAATAGTAAATACTTTCAAGATGAACTCACATATTTGCGCGAATCGGGTAGTGAATTTGCCAAATATCATCCGAA
GTTAACTCACTTTCTCTCTGAAGGAACATTCGACCCAGATGTAGAGCGATTGCTGGAAGGTTTCGCTTTTCTGACTGGAC
GTATTCGAGAGAAGATCGATGACGAACTGCCAGAACTGACGCAATCTCTGATGACTTTGCTGTGGCCGCATTACATGCGT
TCAATTCCATCTTTGTGCATCAGTGAGCTAAAACCCCATACGGGAAGTGTTACTGAGAAAACGGTGGTAAAACGTGGCGC
AGAAATGGCCAGTGAGCAAGTGGAGGGCACGCAATGTCTTTTCCGCACTTGCTACGATGTGAATCTGTACCCAATCACGA
TCACCAACATTGAACAAACCAACAGCCGCACCAGTTCAGCGATTGATGTGACGTTGTCGACGGAGCATGGCCTAGAGCTG
TCTCGAATTGGTTTAGATACGTTGCGTTTGCACTTGCATGGTGAGATTCATATTACCCGCACTGTCTTCCTCTGGCTGTT
CCGTTACTTGGATTACGTGGAGTTGGATGTCGGTGGCGGCTATAAACATCGTTTAGGGCCAGAATATGTTAAGCCGGTAG
GGCATGAAGAAGACGAAGCGCTTCTGCCTTACAGCAAAAATTCATTCGCGGGTTATCGTTTGCTGCAAGAATTATTCTCT
TTGCCAGACAAGTTTATGTTCTTCGACATTACAGGTTTGGAGTGGCTAAAGGGTATTCCACAACGAAGCACAGTGAAAAT
CAAATTTCACTTTAAACGCGCATTGCCGTCAGAGGTTGTACTTAAAGATAAGCACTTAAGGTTGCACTGCACGCCGGCGG
TGAATTTGTTTGAAAAAGATGGTGACCCAATTCGTTTGGAGCACCGTCGTAACGAATACAAAGTCCGTCCTCAAAGTAAT
ACGCAAGAGCACTACGAAGTGTATTCCATTGAGCAAGTTGAGAGTTGGAGCAAAGACGAGCGTCGCCGTAAACCGTTAAT
CGAATTCGAATCGTTCGAGCATCAAATCAACCAACGAGACAAGCGAGAATTCTACAAGTCGAAGGTAGGAGAGCGTGTTA
GTGGAAGAGGGTTAGAGCGCTATATCTCGTTCCACACTCATAATGGTGATATTGCGGATTTAGGTACGGAAACAGTACTC
ATGAAACTGCAATGCAGTAACGCTGATTTGGCCGAACGTCTTTCGGTCGGCGACATTACTTACGCGACCCACAAATCGCC
AACGTATGCGACATTTAAAAACATCACCAAGCCAACACAATCGGTAAGCCCACAGGTAAACGGCGAGCTTCAATGGCAGT
TGATTGCCAATATGTCGCTCAATTATTTGTCTTTGGCAAACATTGATGTATTAAAAGTGCTGCTCTCGACCTATGACTTT
CATTCTCGTGTTGACAGACAAGCTCACCGAGCATCGATTCACCGACTCGATGGCATTGTATCGTCAGAGATGAAACCAAT
TGACCGTGTATTCCGTGGCGTGTCTGTGCGTGGTAACCAGTTCAAGCTAGTAACCAACTCTAAGTTCTTCGTAAATGAAG
GCGATATGTTCCTGATGGCAACGGTACTGAACGAATTCATCCGTTTGTATTCGAGTGTAAACTCCTTTACCGAGCTAGAA
GTATTTGATGAGGCAACCGGCGAAGTATACAACTGGAAAAGCTTAATCGGGCAGCAGACGATTCTATGA					