Detailed information of component protein


Summary

Component ID T6CP015649
Component protein type TssM
T6SS ID (Type) T6SS01247 experimental (Type i1)
Strain Vibrio parahaemolyticus strain T9109
Replicon chromosome
Sequence Protein sequence (1130 a.a.); Nucleotide sequence (3390 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PO79_RS04580
Coordinate (Strand) 214954..218343 (+)
NCBI ID 835943002
RefSeq NZ_JTGR01000038
Uniprot ID UPI0005431024
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1.2e-54 154..408
IcmF-related PF06761.14 7.5e-42 496..755
IcmF_C PF06744.14 6.7e-19 1004..1107
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-11 MFKQKILRNPV
Transmembrane helix 12-34 VVSTIVALVLAAIFAAVYLFILE
Outside 35-38 DADK
Transmembrane helix 39-61 LYLWVGLGVITGFYFTFQLSLWL
Inside 62-401 KKKNSSKVQRLETEEEALSMVIRPLLSNAGKKPIYLMVGNKGAGRRQFLFNSSAIKPMDRSRTAKNDFFEWYESDGAVYVKPDQRLIFQEVSSSDASLWNTFIDEVIRHRPRKPFSGCLFFVDFEFMIVSEPEQKDYTMTALLQRLNSITEKTSSALPVYLMMTKLDKLDGFKEYVHSSSLKTRIEFLSIPLKEAKGVLTEYYLDSFHNLVKVLESNALDASANSTDINEKQAILAFPKQFELCQREISYVLERLCDTNNGVYSLDIREIFFCSSLQGGRKYNLLAKSCSNYFNLPIIASEHTQLTETPYFSRFLLDSQILPESDFAGENKTYLRMIQRQ
Transmembrane helix 402-424 SHLAMLASVVLLAGGTYFFVTTL
Outside 425-1129 DSNLRVINQLLNIDEKVQTEQEASFNQLLQSATRAIQPSYNAWLEGSRALDEEVIPLNISRLNESTKLAYEALIKEVAQQLMPVIEQGYRFELTHNQHDVTVTLPLLKGYLMLKDPTKRDIQFLRRQTLQTLTDLSDKPDVVNQAMRYLDAYFRTQFSPVDINMDVVRATRRSLLANSNVDLVYAGILNQADAIDLGTLDLQRAVGFEFNNVFNEPLDNNRLVIDKIYTSTGFSTFYRPRVDLMSKQVISDNWVLGLSSYVIPTKEEQESFKNQVRKKYTDDYINYWRNALSELKIQSYSNVGDLTNAIDLISGPSSPMTTVLKQVYANTQFSPVGEKNVLISKVDPKLAEVANSASEAVEEVVQPDYLLMKRVEQAFHLLNQLQVSETPNSPTPWDETIAALSRVRTYMKDIADAPDPQMAALAAAQHRMSSSEADPLIKLKQIAQKSPEPVRSWLLDVVKQSWSVMITESAKGIQTQWYSEIYTKFKEIGLGKYPFDLTATEEISIEDFELLFASGGLLDTFIQKNFAPFYDTNLWVPKQVDGETMPLSSALLVQLRNYNVIRDTLINKSTNRVYIPFSAKVLDLDSSAIRASLKISDTDINYYHGPSRIREMEWPPQNGDFNISITIQDVTDEGKQHVLNKSGQWAIYRLLGDSTLTNTHNGSFVSDIKVSGRDLSLRITPLTQKNPFTLAELYNFTLPESI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9980 - -


  Protein sequence: 1130 a.a.    

>T6CP015649 NZ_JTGR01000038:214954-218343 [Vibrio parahaemolyticus] [TssM]
MFKQKILRNPVVVSTIVALVLAAIFAAVYLFILEDADKLYLWVGLGVITGFYFTFQLSLWLKKKNSSKVQRLETEEEALS
MVIRPLLSNAGKKPIYLMVGNKGAGRRQFLFNSSAIKPMDRSRTAKNDFFEWYESDGAVYVKPDQRLIFQEVSSSDASLW
NTFIDEVIRHRPRKPFSGCLFFVDFEFMIVSEPEQKDYTMTALLQRLNSITEKTSSALPVYLMMTKLDKLDGFKEYVHSS
SLKTRIEFLSIPLKEAKGVLTEYYLDSFHNLVKVLESNALDASANSTDINEKQAILAFPKQFELCQREISYVLERLCDTN
NGVYSLDIREIFFCSSLQGGRKYNLLAKSCSNYFNLPIIASEHTQLTETPYFSRFLLDSQILPESDFAGENKTYLRMIQR
QSHLAMLASVVLLAGGTYFFVTTLDSNLRVINQLLNIDEKVQTEQEASFNQLLQSATRAIQPSYNAWLEGSRALDEEVIP
LNISRLNESTKLAYEALIKEVAQQLMPVIEQGYRFELTHNQHDVTVTLPLLKGYLMLKDPTKRDIQFLRRQTLQTLTDLS
DKPDVVNQAMRYLDAYFRTQFSPVDINMDVVRATRRSLLANSNVDLVYAGILNQADAIDLGTLDLQRAVGFEFNNVFNEP
LDNNRLVIDKIYTSTGFSTFYRPRVDLMSKQVISDNWVLGLSSYVIPTKEEQESFKNQVRKKYTDDYINYWRNALSELKI
QSYSNVGDLTNAIDLISGPSSPMTTVLKQVYANTQFSPVGEKNVLISKVDPKLAEVANSASEAVEEVVQPDYLLMKRVEQ
AFHLLNQLQVSETPNSPTPWDETIAALSRVRTYMKDIADAPDPQMAALAAAQHRMSSSEADPLIKLKQIAQKSPEPVRSW
LLDVVKQSWSVMITESAKGIQTQWYSEIYTKFKEIGLGKYPFDLTATEEISIEDFELLFASGGLLDTFIQKNFAPFYDTN
LWVPKQVDGETMPLSSALLVQLRNYNVIRDTLINKSTNRVYIPFSAKVLDLDSSAIRASLKISDTDINYYHGPSRIREME
WPPQNGDFNISITIQDVTDEGKQHVLNKSGQWAIYRLLGDSTLTNTHNGSFVSDIKVSGRDLSLRITPLTQKNPFTLAEL
YNFTLPESI*

  Nucleotide sequence: 3390 bp    

>T6CP015649 NZ_JTGR01000038:214954-218343 [Vibrio parahaemolyticus] [TssM]
ATGTTCAAACAAAAAATACTAAGAAATCCAGTCGTGGTTTCTACCATAGTGGCTTTGGTATTAGCCGCGATATTTGCAGC
GGTATATTTATTCATACTTGAAGACGCAGATAAGCTTTATCTATGGGTTGGTTTAGGAGTTATTACTGGTTTTTATTTTA
CATTCCAGCTTTCCCTATGGCTTAAGAAAAAAAACAGCTCAAAAGTTCAACGTTTAGAGACGGAAGAAGAAGCTCTTTCA
ATGGTGATCCGCCCTCTTCTTTCTAATGCGGGCAAAAAGCCAATCTATCTGATGGTTGGTAATAAAGGGGCCGGTCGACG
TCAGTTTTTGTTTAATTCCAGCGCAATTAAGCCGATGGACCGTTCTCGCACAGCAAAAAACGATTTCTTCGAATGGTATG
AATCTGATGGTGCCGTTTATGTTAAGCCAGATCAACGCCTCATCTTCCAGGAAGTTTCAAGCAGTGATGCTTCTCTCTGG
AATACTTTTATTGATGAAGTGATTCGTCATCGTCCGAGAAAACCCTTTTCAGGTTGCTTATTCTTTGTTGATTTCGAATT
CATGATTGTATCTGAGCCAGAGCAGAAAGATTACACCATGACAGCGCTATTGCAGCGTTTGAACTCTATCACTGAGAAGA
CCTCGTCTGCACTACCTGTTTATTTGATGATGACGAAACTCGACAAACTAGATGGCTTTAAAGAATACGTTCACTCTAGT
TCACTAAAAACACGTATTGAATTTCTGTCCATTCCATTGAAGGAAGCGAAAGGTGTACTTACCGAATACTACCTAGATAG
CTTCCATAATCTGGTAAAAGTGCTAGAAAGCAACGCTTTAGACGCGTCGGCAAACTCAACAGATATCAACGAAAAGCAAG
CCATCCTTGCCTTCCCAAAACAGTTTGAACTCTGTCAACGTGAAATCAGCTACGTGCTTGAACGTCTTTGCGATACAAAC
AATGGCGTTTACTCGTTGGATATTCGCGAAATCTTCTTCTGTTCTTCTTTGCAAGGTGGACGCAAGTACAACTTGTTAGC
CAAAAGCTGCAGTAATTACTTCAATTTACCAATTATCGCATCTGAGCACACGCAACTAACTGAAACACCGTACTTTTCTC
GCTTCTTGCTTGACTCTCAAATCCTCCCAGAGAGTGACTTCGCAGGCGAGAACAAGACTTACCTACGCATGATTCAGCGT
CAAAGCCATTTGGCAATGCTGGCATCCGTTGTCTTATTGGCAGGCGGTACTTATTTTTTTGTCACAACACTAGACAGTAA
CTTGCGTGTTATTAACCAATTGCTGAATATCGACGAAAAAGTTCAAACAGAACAAGAAGCAAGCTTCAACCAGTTATTGC
AGAGTGCTACTCGTGCTATCCAACCCTCATACAATGCTTGGTTAGAAGGATCACGAGCATTAGATGAAGAAGTAATACCG
CTAAATATAAGCAGGTTAAACGAAAGCACAAAATTAGCGTATGAAGCATTGATTAAAGAGGTTGCTCAACAACTTATGCC
TGTTATTGAGCAAGGCTACCGTTTTGAACTGACACACAACCAACATGATGTCACAGTAACGTTGCCGTTATTAAAAGGCT
ACTTGATGTTGAAGGATCCTACCAAGCGAGATATTCAGTTTTTACGACGCCAAACATTGCAAACACTGACAGACTTGAGC
GATAAGCCGGATGTGGTAAATCAAGCTATGCGATACTTGGACGCCTACTTCCGCACTCAATTCTCACCTGTAGATATCAA
CATGGATGTCGTTCGAGCAACTCGTCGCTCATTGTTGGCAAATTCAAATGTGGATCTTGTTTACGCAGGCATTTTAAACC
AAGCAGATGCTATCGATCTTGGCACACTCGATCTACAACGTGCAGTTGGCTTTGAATTTAACAACGTATTTAACGAGCCG
TTAGATAACAACCGACTAGTAATCGATAAGATTTATACCTCCACTGGCTTTAGTACCTTCTATCGCCCTCGCGTCGACTT
GATGTCTAAACAAGTGATCAGTGATAACTGGGTGCTAGGATTGTCCAGCTATGTTATCCCAACCAAAGAAGAGCAAGAAT
CGTTTAAAAATCAAGTACGTAAAAAGTACACCGATGATTATATCAACTACTGGCGCAACGCTCTCAGTGAGTTAAAAATT
CAGAGTTATAGCAATGTGGGTGATCTAACAAATGCTATCGACCTAATCTCTGGACCATCTTCACCAATGACGACTGTATT
AAAGCAAGTCTATGCAAATACTCAGTTTTCACCGGTAGGAGAAAAAAACGTACTCATCTCGAAAGTAGACCCAAAACTGG
CAGAAGTGGCGAACTCTGCGTCAGAAGCGGTTGAAGAAGTGGTTCAGCCGGATTACTTGTTGATGAAACGTGTAGAACAA
GCGTTCCATCTTCTAAATCAGCTTCAAGTAAGCGAGACTCCTAACTCCCCTACTCCATGGGACGAAACTATCGCAGCTTT
AAGCCGGGTGCGCACTTACATGAAGGATATTGCGGATGCTCCCGATCCACAAATGGCAGCACTTGCAGCCGCTCAACACC
GAATGAGCAGCTCTGAAGCCGATCCGCTAATTAAACTTAAACAGATTGCTCAGAAGTCACCAGAGCCAGTACGCAGTTGG
TTACTTGATGTCGTAAAGCAAAGCTGGTCGGTAATGATCACCGAGTCAGCAAAAGGCATTCAAACCCAATGGTATAGTGA
AATTTACACCAAATTTAAAGAGATAGGACTTGGTAAATACCCATTTGATCTCACAGCAACAGAGGAGATTTCTATCGAAG
ACTTCGAACTGTTGTTTGCTTCAGGTGGGTTATTAGACACATTTATTCAGAAAAACTTCGCACCGTTCTACGATACCAAC
TTGTGGGTACCAAAACAGGTAGATGGTGAAACCATGCCACTATCTTCAGCCTTACTGGTGCAATTGCGCAATTACAACGT
TATCCGCGATACGCTGATTAATAAGAGTACTAACCGAGTTTATATTCCATTCAGTGCGAAAGTATTAGATCTTGACTCAA
GTGCTATCAGAGCCAGTTTGAAAATCTCTGACACTGATATCAACTACTATCACGGTCCGAGCCGAATTCGTGAAATGGAG
TGGCCACCGCAAAACGGCGACTTCAACATTAGTATCACGATCCAAGATGTGACAGACGAAGGTAAACAACACGTTTTAAA
TAAGAGTGGTCAATGGGCGATATATAGATTGCTAGGTGATTCGACCCTGACAAACACTCATAACGGTAGCTTCGTTAGTG
ACATAAAAGTATCAGGGAGGGATTTGAGCCTCCGTATAACTCCCTTGACACAAAAAAACCCTTTCACTTTGGCTGAGTTG
TACAACTTTACGCTCCCAGAGTCGATTTGA