Detailed information of component protein


Summary

Component ID T6CP015648
Component protein type TagA
T6SS ID (Type) T6SS01247 experimental (Type i1)
Strain Vibrio parahaemolyticus strain T9109
Replicon chromosome
Sequence Protein sequence (432 a.a.); Nucleotide sequence (1296 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PO79_RS04575
Coordinate (Strand) 213637..214932 (+)
NCBI ID 646710470
RefSeq NZ_JTGR01000038
Uniprot ID UPI0004139B89
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 2e-24 9..119
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-230 MFFSDFTRRPIDESNPSGTNPNGLDDFDEIKRQINGLNKVTGRVSWKTVQTLSKEILRTKGKDFRCSCYFTVAAINNDGLKGLVEGLNSVLDLCVVYWFSAYPEYTKANARISAIEWMVEYTEKRMKKHRVLPEELPLIEAAHQLCLRIEEELRLHYGIKAPSFGRIRRPLNQWIEEIKEEKTKQEKTSQTKPASNPPSAGIRVEIAPPNRPVQKKVEQPKVEEEAKSSR
Transmembrane helix 231-250 FIVYTMIGLLIIAFTSHFIY
Inside 251-431 KQHQYTILKERIGSASLTELVTIVNSLSVENKNYQDDLRSVTVERLHTLMSGWTLDPVKVSQVNTIEQLTNDLTKLYPDSSSAQQLRDNFLGQRSYFEDEFEAIYKRFAYARTVFANIAKQSADKDAKKAYEYSNSLFPLLGRIEYAEKNSQRKEIERSLLLLNAYLYKINQLQAAEKDNK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9452 - -


  Protein sequence: 432 a.a.    

>T6CP015648 NZ_JTGR01000038:213637-214932 [Vibrio parahaemolyticus] [TagA]
MFFSDFTRRPIDESNPSGTNPNGLDDFDEIKRQINGLNKVTGRVSWKTVQTLSKEILRTKGKDFRCSCYFTVAAINNDGL
KGLVEGLNSVLDLCVVYWFSAYPEYTKANARISAIEWMVEYTEKRMKKHRVLPEELPLIEAAHQLCLRIEEELRLHYGIK
APSFGRIRRPLNQWIEEIKEEKTKQEKTSQTKPASNPPSAGIRVEIAPPNRPVQKKVEQPKVEEEAKSSRFIVYTMIGLL
IIAFTSHFIYKQHQYTILKERIGSASLTELVTIVNSLSVENKNYQDDLRSVTVERLHTLMSGWTLDPVKVSQVNTIEQLT
NDLTKLYPDSSSAQQLRDNFLGQRSYFEDEFEAIYKRFAYARTVFANIAKQSADKDAKKAYEYSNSLFPLLGRIEYAEKN
SQRKEIERSLLLLNAYLYKINQLQAAEKDNK*

  Nucleotide sequence: 1296 bp    

>T6CP015648 NZ_JTGR01000038:213637-214932 [Vibrio parahaemolyticus] [TagA]
ATGTTTTTCAGCGACTTCACAAGAAGACCGATCGATGAAAGTAATCCAAGCGGTACCAATCCAAATGGCTTAGACGACTT
TGATGAAATAAAACGTCAAATAAATGGCTTGAACAAAGTAACAGGCCGTGTGTCATGGAAGACAGTTCAGACACTATCAA
AAGAAATCCTTAGAACGAAAGGCAAAGACTTTCGCTGTAGCTGTTACTTTACTGTGGCAGCCATTAATAATGACGGACTA
AAAGGCCTAGTTGAGGGCTTAAATTCTGTGCTCGACTTGTGTGTTGTTTATTGGTTCTCCGCCTATCCTGAGTACACAAA
AGCTAATGCCCGTATTAGTGCGATTGAATGGATGGTTGAGTACACTGAAAAACGAATGAAGAAACACCGAGTCCTGCCGG
AAGAACTCCCCCTAATTGAAGCGGCTCATCAGCTTTGTCTACGTATCGAAGAGGAACTTCGCCTTCACTATGGTATTAAG
GCACCTTCTTTTGGACGTATTCGTCGTCCCCTAAATCAGTGGATTGAAGAGATTAAAGAAGAAAAGACGAAACAAGAGAA
AACCTCACAAACAAAACCTGCGTCAAATCCTCCTTCAGCAGGCATCCGAGTCGAAATAGCACCGCCCAATAGACCTGTTC
AAAAGAAAGTAGAGCAGCCTAAAGTGGAAGAAGAGGCCAAATCAAGCCGATTCATTGTCTATACAATGATCGGATTGCTG
ATCATAGCTTTTACGTCTCACTTCATATACAAACAGCATCAATACACAATACTCAAGGAAAGAATTGGGTCAGCATCACT
TACTGAATTAGTGACGATTGTTAACTCGCTCAGTGTCGAAAACAAAAATTACCAAGATGACTTGCGCTCTGTCACGGTTG
AGCGTCTTCATACTTTGATGTCAGGTTGGACATTAGATCCAGTCAAAGTGAGCCAGGTCAATACCATTGAACAGTTAACG
AATGACCTGACCAAGCTCTATCCAGACTCATCCTCTGCACAACAACTTCGCGATAATTTTTTAGGCCAACGTTCTTATTT
TGAAGATGAATTTGAGGCTATATACAAACGTTTTGCATATGCTAGAACGGTCTTTGCCAACATTGCAAAACAAAGTGCAG
ACAAGGACGCTAAGAAAGCATATGAGTACAGCAATTCGCTATTTCCCCTTCTTGGGCGAATTGAATATGCAGAAAAGAAC
TCTCAGAGAAAGGAAATTGAACGATCACTCCTTTTGCTGAACGCTTACCTATATAAAATCAACCAGCTACAAGCTGCAGA
AAAAGACAATAAATAA