SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015646
Component protein type	TssJ
T6SS ID (Type)	T6SS01247 (Type i1)
Strain	Vibrio parahaemolyticus strain T9109
Replicon	chromosome
Sequence	Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Reference	[1] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PO79_RS04560
Coordinate (Strand)	210501..211001 (-)
NCBI ID	499418365
RefSeq	NZ_JTGR01000038
Uniprot ID	Q87PT9_VIBPA, A0A227JG17_VIBPH
KEGG ID	vpa:VP1412
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	6.7e-31	34..150

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-166	MRSIAILLGCALLSGCAMWDQFKESTGITPETSSIELVIEASSVLNVREGGQSSPVILRVHELTSPVLFRSLDFFALFENDKASLGDEYIKRYEYQMQPGEKIHEFLELDPATRAVGFSVAFRDIDGSSWRKVEVIEEKSEYYIKLKLEGSELISDNTRGIEQVYF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9975	pos: 15-16. LSG-CA.	MRSIAILLGCALLSG

Protein sequence: 167 a.a.

						>T6CP015646 NZ_JTGR01000038:c211001-210501 [Vibrio parahaemolyticus] [TssJ]
MRSIAILLGCALLSGCAMWDQFKESTGITPETSSIELVIEASSVLNVREGGQSSPVILRVHELTSPVLFRSLDFFALFEN
DKASLGDEYIKRYEYQMQPGEKIHEFLELDPATRAVGFSVAFRDIDGSSWRKVEVIEEKSEYYIKLKLEGSELISDNTRG
IEQVYF*					

Nucleotide sequence: 501 bp

						>T6CP015646 NZ_JTGR01000038:c211001-210501 [Vibrio parahaemolyticus] [TssJ]
ATGCGTAGTATTGCAATATTGCTCGGATGCGCCTTGCTATCCGGTTGTGCCATGTGGGATCAATTTAAAGAATCAACAGG
CATCACGCCTGAAACGTCCTCGATTGAGTTGGTGATTGAGGCATCTTCGGTTCTAAATGTTCGTGAAGGCGGACAATCCT
CCCCGGTTATTTTGCGAGTTCACGAACTGACGTCACCAGTGTTGTTTCGTAGCTTAGACTTCTTCGCATTGTTTGAGAAC
GATAAAGCATCACTGGGTGATGAATACATCAAACGTTATGAATACCAAATGCAGCCCGGTGAAAAGATCCACGAATTTTT
GGAACTCGACCCAGCAACCCGTGCGGTTGGATTCTCAGTCGCGTTTCGCGATATTGACGGTTCTTCTTGGCGTAAAGTCG
AAGTGATTGAAGAGAAGAGCGAGTACTACATAAAACTCAAGTTAGAAGGCAGTGAGTTAATTTCTGACAACACTCGCGGC
ATTGAACAAGTTTATTTTTAA					