SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015587
Component protein type	TssG
T6SS ID (Type)	T6SS01243 (Type i5)
Strain	Agrobacterium fabrum strain 12D13
Replicon	chromosome
Sequence	Protein sequence (335 a.a.); Nucleotide sequence (1005 bp)
Reference	[1] Wu CF, Santos MNM, Cho ST, et al. Plant-Pathogenic Agrobacterium tumefaciens Strains Have Diverse Type VI Effector-Immunity Pairs and Vary in In-Planta Competitiveness. Mol Plant Microbe Interact. 2019 Aug;32(8):961-971. doi: 10.1094/MPMI-01-19-0021-R. Epub 2019 Jun 27. PMID: 30830835

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	At12D13_RS20920
Coordinate (Strand)	1429102..1430106 (-)
NCBI ID	WP_121691713.1
RefSeq	NZ_CP033035
Uniprot ID	UPI000EF59B2F
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	6e-61	59..309

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-334	MDQQIPTMKDRQAALLSDDPGRFEPTTAFRVAQFVAADDGVVIGAHGGTQPTPLAVSGFNHKRGYAELKSAFAPIVGPLGSLPPAYGELLQREERNRSGALASFFNLFAARFSELFVSATEKYRLARGLRWSAREQDNGFRKTLLALTGFRTKGLAQKAGVAEDTLLRFSGLLASRNRNVSALTSMLCEFTALPVTVEQFRRRWVALPLHEQSQLGQASGLRLGQNTTAGSAVEDMSGGFRVVIGPVGYADYLALTPGSKRLAEIFALTRLFVGSALHFDVQVILKKEDIPFCRLGQSDAPARLGWNSWARVAPAEKDSADAVVTEREGSAISV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9778	-	-

Protein sequence: 335 a.a.

						>T6CP015587 NZ_CP033035:c1430106-1429102 [Agrobacterium fabrum] [TssG]
MDQQIPTMKDRQAALLSDDPGRFEPTTAFRVAQFVAADDGVVIGAHGGTQPTPLAVSGFNHKRGYAELKSAFAPIVGPLG
SLPPAYGELLQREERNRSGALASFFNLFAARFSELFVSATEKYRLARGLRWSAREQDNGFRKTLLALTGFRTKGLAQKAG
VAEDTLLRFSGLLASRNRNVSALTSMLCEFTALPVTVEQFRRRWVALPLHEQSQLGQASGLRLGQNTTAGSAVEDMSGGF
RVVIGPVGYADYLALTPGSKRLAEIFALTRLFVGSALHFDVQVILKKEDIPFCRLGQSDAPARLGWNSWARVAPAEKDSA
DAVVTEREGSAISV*					

Nucleotide sequence: 1005 bp

						>T6CP015587 NZ_CP033035:c1430106-1429102 [Agrobacterium fabrum] [TssG]
ATGGACCAGCAAATCCCAACCATGAAAGACCGGCAGGCCGCGCTTCTCAGCGACGATCCCGGCCGTTTCGAACCCACCAC
CGCGTTTCGCGTCGCCCAATTCGTCGCGGCGGATGACGGCGTCGTCATCGGTGCTCATGGCGGCACACAGCCGACACCGC
TCGCGGTCAGCGGTTTTAATCACAAGCGCGGCTATGCCGAACTGAAATCCGCTTTCGCCCCGATCGTCGGTCCGCTCGGC
TCGTTGCCGCCGGCCTATGGCGAATTGCTGCAACGGGAAGAACGTAATCGTTCCGGTGCGCTCGCGAGCTTCTTCAATCT
GTTCGCGGCAAGGTTCAGCGAGCTTTTCGTCTCCGCAACGGAGAAATATCGCCTCGCAAGAGGCCTGCGCTGGTCGGCCA
GGGAACAGGATAATGGTTTCCGCAAAACCCTGCTGGCGCTGACGGGGTTCAGGACGAAAGGACTGGCACAGAAGGCCGGC
GTTGCCGAAGACACACTTCTGCGTTTCAGCGGGCTGTTGGCCAGCCGCAACCGCAATGTCAGCGCGCTCACCTCCATGCT
GTGCGAGTTCACCGCGCTTCCGGTCACGGTCGAACAGTTTCGGCGGCGCTGGGTGGCTTTGCCGCTTCACGAACAAAGCC
AGCTCGGACAGGCCTCCGGACTGAGGCTGGGGCAGAACACAACGGCAGGAAGCGCTGTCGAAGATATGAGCGGCGGCTTC
CGGGTGGTGATCGGCCCCGTCGGCTATGCCGACTATCTGGCCCTGACGCCCGGATCGAAGCGGCTTGCGGAAATATTCGC
GCTGACGCGCCTGTTCGTTGGAAGCGCGCTGCATTTCGATGTGCAGGTGATCCTGAAAAAAGAGGACATTCCCTTTTGCC
GCCTCGGCCAGTCCGATGCGCCCGCCCGTCTCGGCTGGAACAGCTGGGCCCGCGTGGCACCCGCAGAAAAAGACAGCGCC
GATGCTGTTGTGACCGAGCGCGAAGGCTCGGCTATTTCCGTGTGA					