Detailed information of component protein


Summary

Component ID T6CP015585
Component protein type TssL
T6SS ID (Type) T6SS01243 experimental (Type i5)
Strain Agrobacterium fabrum strain 12D13
Replicon chromosome
Sequence Protein sequence (502 a.a.); Nucleotide sequence (1506 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) At12D13_RS20905
Coordinate (Strand) 1425060..1426565 (-)
NCBI ID WP_121691711.1
RefSeq NZ_CP033035
Uniprot ID UPI000EF5DE39
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2.1e-55 75..276
OmpA PF00691.22 4.7e-12 367..464
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-255 MSTENPSSWQDLPTVVEITEESRRRNQNARNMAAVLEDIVETDEQARPARGKEATLPVDKLLTGFHFGGEEVPTLVQSAAPLLNLAHLLRFSDAEPDPDQLRRACVEAITRYERDLASARISPERARAAHYVVCATVDDVVLSKPWGVRAGWARSGLVSTFHNDVTGGDRVFDILDHFHQSPGANKDLLLLIYLCLSLAFEGRTRVSSKGALELSRIRDSLYKTLIGQYGTFERELSPHWKGVDARHTPLRTMTA
Transmembrane helix 256-278 LWTLLSLMALAFALGYLFFTLSL
Outside 279-501 NNASDVTFERLAHLPPTETPSVLIANPPPQPPAQVEIPRIVEPPKTVAPRQPSRLEKLMAFLQPEVDKKLVTLADVNGRLRVRINNSGLFTTGSADVSPQFRDLIQRIGGALAAEKFRAVVIGYTDTVPIKTVEFPSNWHLSEARAKAVGDILSQFTGPDAILTEGRADSDPIADNKTEEGRQINRRTEIMVLTNPDEKLSDAGILSPPIENSSGQQPGEAQP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9813 - -


  Protein sequence: 502 a.a.    

>T6CP015585 NZ_CP033035:c1426565-1425060 [Agrobacterium fabrum] [TssL]
MSTENPSSWQDLPTVVEITEESRRRNQNARNMAAVLEDIVETDEQARPARGKEATLPVDKLLTGFHFGGEEVPTLVQSAA
PLLNLAHLLRFSDAEPDPDQLRRACVEAITRYERDLASARISPERARAAHYVVCATVDDVVLSKPWGVRAGWARSGLVST
FHNDVTGGDRVFDILDHFHQSPGANKDLLLLIYLCLSLAFEGRTRVSSKGALELSRIRDSLYKTLIGQYGTFERELSPHW
KGVDARHTPLRTMTALWTLLSLMALAFALGYLFFTLSLNNASDVTFERLAHLPPTETPSVLIANPPPQPPAQVEIPRIVE
PPKTVAPRQPSRLEKLMAFLQPEVDKKLVTLADVNGRLRVRINNSGLFTTGSADVSPQFRDLIQRIGGALAAEKFRAVVI
GYTDTVPIKTVEFPSNWHLSEARAKAVGDILSQFTGPDAILTEGRADSDPIADNKTEEGRQINRRTEIMVLTNPDEKLSD
AGILSPPIENSSGQQPGEAQP*

  Nucleotide sequence: 1506 bp    

>T6CP015585 NZ_CP033035:c1426565-1425060 [Agrobacterium fabrum] [TssL]
ATGAGCACGGAAAACCCCTCTTCCTGGCAGGATTTGCCGACGGTGGTCGAGATCACCGAGGAAAGCCGCCGGCGCAACCA
GAACGCCCGCAACATGGCGGCAGTCCTCGAAGACATCGTCGAGACCGACGAGCAGGCTCGCCCCGCCAGGGGGAAAGAGG
CCACCCTGCCGGTCGACAAGCTGCTGACCGGTTTCCACTTCGGCGGAGAGGAGGTGCCGACACTTGTGCAATCGGCGGCT
CCCCTTCTCAACCTCGCACATCTGCTGCGCTTCAGCGATGCCGAGCCGGACCCCGACCAGCTTCGCCGCGCCTGCGTTGA
GGCGATCACCCGTTACGAGCGCGATCTCGCTTCCGCCCGCATCAGCCCCGAGCGGGCAAGAGCAGCGCATTACGTCGTCT
GCGCAACAGTGGATGATGTCGTTCTCAGCAAGCCCTGGGGCGTGCGGGCAGGCTGGGCGCGCTCCGGCCTCGTTTCCACC
TTCCACAATGACGTGACCGGTGGCGACCGGGTTTTCGACATTCTCGACCATTTCCACCAGTCGCCCGGCGCCAACAAGGA
TCTGCTGCTGCTGATCTATCTGTGCCTGTCGCTGGCTTTCGAAGGACGTACCCGCGTTTCATCGAAAGGGGCACTGGAGC
TTTCGCGCATCCGCGACAGCCTCTACAAGACGCTGATCGGCCAATATGGCACCTTCGAGCGGGAGCTTTCCCCACATTGG
AAAGGCGTCGATGCCCGTCATACGCCACTGCGGACGATGACCGCGCTGTGGACCCTGCTGTCGCTGATGGCACTCGCCTT
CGCGCTAGGTTATCTCTTCTTCACGCTATCGCTCAACAACGCATCCGATGTCACCTTCGAAAGGCTTGCCCACCTGCCGC
CGACCGAGACGCCGAGCGTGCTGATCGCCAACCCGCCGCCCCAGCCACCGGCGCAAGTCGAAATTCCCAGGATTGTCGAG
CCGCCGAAGACGGTGGCGCCGAGACAACCTTCCCGGCTTGAAAAGCTGATGGCCTTCCTGCAGCCGGAAGTCGACAAGAA
GCTTGTGACCCTGGCGGATGTGAACGGGCGGCTGCGAGTTCGCATCAACAATTCGGGCCTGTTCACGACAGGCAGCGCCG
ATGTCAGCCCGCAGTTCCGCGACCTCATCCAGCGTATCGGCGGTGCGCTTGCGGCTGAAAAATTCCGCGCCGTCGTCATC
GGTTATACCGACACCGTGCCGATCAAGACGGTGGAGTTTCCATCGAACTGGCACCTTTCCGAGGCGCGCGCCAAGGCGGT
CGGAGACATTCTTTCACAATTCACCGGCCCCGACGCGATCCTGACCGAAGGCCGGGCCGACAGCGACCCGATCGCCGACA
ACAAAACGGAAGAAGGCCGCCAGATAAATCGCAGAACAGAAATCATGGTCCTGACCAATCCCGATGAAAAACTGAGCGAC
GCCGGCATTCTCTCGCCGCCCATAGAGAACAGTTCGGGACAGCAGCCGGGAGAGGCCCAGCCATGA