Detailed information of component protein


Summary

Component ID T6CP015539
Component protein type TssM
T6SS ID (Type) T6SS01240 experimental (Type i5)
Strain Agrobacterium fabrum strain 1D132
Replicon chromosome
Sequence Protein sequence (1160 a.a.); Nucleotide sequence (3480 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) At1D132_RS21320
Coordinate (Strand) 1431242..1434721 (-)
NCBI ID WP_092772437.1
RefSeq NZ_CP033023
Uniprot ID UPI000884CEA3
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 2.3e-78 203..442
IcmF-related PF06761.14 1.2e-60 496..793
IcmF_C PF06744.14 6.6e-18 1037..1140
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-24 MNPLSYFYTIRSYVESYAALVGRR
Transmembrane helix 25-47 FLSLLWVIALCVVIWFYGYLIAL
Outside 48-56 GDFKPLGTV
Transmembrane helix 57-79 QARLIAIGIIVAVWLVYIIVTIY
Inside 80-432 RGRKQDKELVDSIEREALANRQAEIGEIQTRLKEALALLRRVTKKRFGYIYDLPWYVIFGAPGSGKTTALTNSGLQFPLGDALGENAVKGIGGTRNCNWWFADEAILIDTAGRYTTQDDLDGSSKAGWEGFLGLLRRYRRSQPINGALVTLSIPDLLNRDPEEQRQELRSIRQRLSELDEYLHARVPVYIVLTKADLLHGFVEFFDGFNKTDRQQVWGTTFKLDESYSAENLPQRLTEEFELLQQRVDAMLIERLQQEQNAEIRGRIFRFPAELARLKDRLHEALAELCASSPLIEAPLLRGVYFASGTQPETEKSPAASRTRRSYFLSRLFKDVIFPEAALVTRDKRLSRRQ
Transmembrane helix 433-455 LLVRRIAYAVSATAVAIVFTGWI
Outside 456-1159 FTYFANTQALAEADRKLGAYEQLVQGIPVREVADADFLRILPALDNLRDVNSGFARERVWNVSFGLDQENKIAGRQRDAYQRALNALLLPRMIVQLQKQLKDEKDVTRTFNSLKLYGMLGGMGALDRDFLTTQTHQMFASLYPGDGRAAAREALDQHAKALADGVLAPIELDARLIATARETIRDQAIGTRAYDILAGLPQVRELMEWTPATAFGPLGERAFERRSKAPMAEGIEGLFTANGYRRVVIPQVAHAARIALSEDWVRGSDDAIKGATVEQVAQAALQIYFDRFEKIWADTLSDLRVKPSQSLGDAVETTRALANERNIVVEAARSIAEATDLRPGANPAALASAAEGDATAAVLAATVNAADPYARLRDMLATKGAATTAEQPNGDKTGGSPSEQLLAHFKLLNEQLARSATTSDEVAKVFDVDSQLTKANQDLLQQARELPAPLDVWVAGVAADVGSLAVKSARSRIAELWTADSASLCSSIVTGRYPFDRASSRDVAIADFTRLFAPTGVFQSFFKQRMEPFVDKTTTPWSWKGTFGATGIPSSAVAQFENADKISRAFFPNGSETPAVSINVKPVSLTNASSAVMLEIEGERVVYYHGPIQAKSITWPSRENTASLSRIAFQPGGWQQAKTENGDWSPFRLFDGANIENQSGELLRVRFENGVQAAEFDIQFGSVLNPFKLDAIASFACPAQF


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9908 - -


  Protein sequence: 1160 a.a.    

>T6CP015539 NZ_CP033023:c1434721-1431242 [Agrobacterium fabrum] [TssM]
MNPLSYFYTIRSYVESYAALVGRRFLSLLWVIALCVVIWFYGYLIALGDFKPLGTVQARLIAIGIIVAVWLVYIIVTIYR
GRKQDKELVDSIEREALANRQAEIGEIQTRLKEALALLRRVTKKRFGYIYDLPWYVIFGAPGSGKTTALTNSGLQFPLGD
ALGENAVKGIGGTRNCNWWFADEAILIDTAGRYTTQDDLDGSSKAGWEGFLGLLRRYRRSQPINGALVTLSIPDLLNRDP
EEQRQELRSIRQRLSELDEYLHARVPVYIVLTKADLLHGFVEFFDGFNKTDRQQVWGTTFKLDESYSAENLPQRLTEEFE
LLQQRVDAMLIERLQQEQNAEIRGRIFRFPAELARLKDRLHEALAELCASSPLIEAPLLRGVYFASGTQPETEKSPAASR
TRRSYFLSRLFKDVIFPEAALVTRDKRLSRRQLLVRRIAYAVSATAVAIVFTGWIFTYFANTQALAEADRKLGAYEQLVQ
GIPVREVADADFLRILPALDNLRDVNSGFARERVWNVSFGLDQENKIAGRQRDAYQRALNALLLPRMIVQLQKQLKDEKD
VTRTFNSLKLYGMLGGMGALDRDFLTTQTHQMFASLYPGDGRAAAREALDQHAKALADGVLAPIELDARLIATARETIRD
QAIGTRAYDILAGLPQVRELMEWTPATAFGPLGERAFERRSKAPMAEGIEGLFTANGYRRVVIPQVAHAARIALSEDWVR
GSDDAIKGATVEQVAQAALQIYFDRFEKIWADTLSDLRVKPSQSLGDAVETTRALANERNIVVEAARSIAEATDLRPGAN
PAALASAAEGDATAAVLAATVNAADPYARLRDMLATKGAATTAEQPNGDKTGGSPSEQLLAHFKLLNEQLARSATTSDEV
AKVFDVDSQLTKANQDLLQQARELPAPLDVWVAGVAADVGSLAVKSARSRIAELWTADSASLCSSIVTGRYPFDRASSRD
VAIADFTRLFAPTGVFQSFFKQRMEPFVDKTTTPWSWKGTFGATGIPSSAVAQFENADKISRAFFPNGSETPAVSINVKP
VSLTNASSAVMLEIEGERVVYYHGPIQAKSITWPSRENTASLSRIAFQPGGWQQAKTENGDWSPFRLFDGANIENQSGEL
LRVRFENGVQAAEFDIQFGSVLNPFKLDAIASFACPAQF*

  Nucleotide sequence: 3480 bp    

>T6CP015539 NZ_CP033023:c1434721-1431242 [Agrobacterium fabrum] [TssM]
ATGAATCCATTGAGCTATTTTTATACGATCCGTTCCTATGTCGAGAGCTATGCGGCCCTCGTCGGCCGGCGTTTCCTCTC
GCTTTTATGGGTGATCGCGCTCTGCGTCGTCATCTGGTTCTACGGCTATTTGATCGCGCTCGGCGATTTCAAACCGCTTG
GAACGGTGCAGGCGCGGCTCATCGCCATCGGCATCATCGTCGCCGTCTGGCTGGTCTACATCATCGTCACGATCTACCGC
GGCCGCAAACAGGACAAGGAACTCGTCGACAGTATCGAGCGCGAGGCGCTGGCCAATCGGCAGGCGGAAATCGGCGAAAT
CCAGACTCGCCTGAAAGAAGCGCTCGCGCTCTTGCGGCGCGTCACGAAAAAGCGCTTCGGTTACATTTACGACTTGCCCT
GGTACGTGATCTTCGGCGCACCCGGCTCCGGGAAGACGACGGCGCTGACCAATTCCGGCCTGCAATTTCCGCTTGGCGAT
GCGCTCGGTGAAAATGCGGTGAAGGGTATCGGTGGTACGCGCAACTGTAACTGGTGGTTCGCGGATGAGGCGATCCTTAT
CGACACTGCCGGTCGCTATACCACCCAGGATGACCTCGACGGTTCCTCGAAGGCCGGCTGGGAAGGCTTTCTTGGCCTTT
TGAGGCGATATCGCCGTTCGCAGCCGATCAATGGCGCATTGGTCACCCTGTCCATTCCCGATCTGCTCAACCGCGATCCC
GAAGAACAACGGCAGGAGCTGCGCTCCATCCGCCAGCGTCTTTCGGAACTTGACGAATATCTGCATGCCCGCGTTCCGGT
CTATATCGTCCTGACGAAAGCCGACCTGCTGCATGGTTTCGTGGAATTTTTCGATGGATTCAACAAGACCGACCGCCAGC
AGGTGTGGGGCACCACCTTCAAGCTGGATGAGAGCTACTCCGCCGAAAACCTGCCGCAGCGCCTGACGGAAGAATTCGAA
CTGCTACAGCAGCGGGTCGATGCCATGCTGATCGAACGCCTGCAGCAGGAGCAGAATGCGGAGATACGCGGCCGTATCTT
CCGTTTTCCAGCCGAACTCGCGCGGCTGAAAGACCGCCTGCACGAGGCCCTTGCCGAACTTTGCGCAAGTTCGCCTCTCA
TCGAGGCGCCGCTTCTGCGCGGCGTTTATTTCGCGTCGGGAACGCAGCCGGAAACGGAAAAATCTCCGGCGGCATCCAGA
ACCCGCCGCAGCTATTTCCTGTCGCGGTTGTTCAAGGACGTCATCTTCCCTGAAGCCGCCCTGGTGACGCGTGACAAGCG
CCTTTCCCGGCGGCAATTGCTGGTGCGGCGCATTGCTTATGCCGTATCCGCCACGGCGGTCGCCATCGTCTTCACCGGCT
GGATTTTCACCTATTTCGCCAATACGCAGGCGCTGGCCGAAGCGGATCGCAAGCTTGGCGCTTATGAACAGCTCGTTCAG
GGAATTCCCGTGCGCGAGGTCGCGGACGCCGATTTCCTGCGGATCCTCCCCGCGCTCGACAATCTGCGCGATGTCAATTC
CGGCTTTGCCCGCGAACGCGTGTGGAATGTCAGCTTCGGTCTTGATCAGGAAAACAAGATTGCCGGCCGGCAGCGTGACG
CCTATCAGCGCGCACTCAACGCCCTTCTGCTGCCGCGCATGATCGTGCAGCTGCAAAAGCAGCTGAAGGACGAAAAGGAT
GTCACCCGCACGTTCAATTCGCTGAAACTCTACGGAATGCTGGGCGGGATGGGTGCACTCGACCGCGATTTCCTGACCAC
GCAGACGCATCAGATGTTCGCATCCCTTTATCCCGGTGACGGAAGGGCGGCGGCGAGAGAAGCGCTTGACCAGCATGCAA
AGGCGCTCGCTGACGGCGTGCTCGCCCCGATCGAGCTCGATGCGCGGCTCATCGCCACCGCCCGCGAAACCATCCGCGAT
CAGGCGATTGGCACACGCGCCTATGATATTCTCGCCGGCCTTCCGCAAGTGCGGGAATTGATGGAATGGACACCAGCTAC
GGCTTTCGGCCCGCTGGGGGAGCGTGCTTTCGAGCGTCGCAGCAAAGCGCCGATGGCCGAGGGGATAGAGGGCCTTTTCA
CCGCCAATGGATATCGCCGCGTCGTCATCCCGCAGGTCGCGCATGCGGCCCGCATCGCGCTTTCCGAAGACTGGGTGCGC
GGTTCTGACGATGCCATCAAGGGCGCAACTGTCGAGCAGGTGGCGCAGGCCGCGCTGCAAATCTATTTCGACAGGTTCGA
GAAAATATGGGCCGACACGCTGTCCGATCTGCGCGTCAAACCATCCCAGAGCCTCGGCGACGCGGTGGAGACAACACGGG
CTCTTGCCAATGAGCGCAATATCGTGGTGGAGGCTGCCAGATCGATCGCAGAGGCGACCGATCTCCGCCCCGGAGCAAAC
CCCGCCGCCCTCGCCTCCGCTGCGGAAGGAGACGCAACCGCAGCCGTTCTGGCCGCTACGGTGAATGCGGCCGATCCCTA
TGCGAGACTGCGCGACATGCTGGCGACGAAAGGCGCAGCCACAACGGCCGAGCAACCAAATGGCGACAAGACCGGCGGTT
CGCCATCGGAACAATTACTCGCGCATTTCAAGCTGCTGAACGAACAGCTTGCCCGTTCGGCAACCACCTCCGATGAGGTC
GCGAAAGTGTTCGACGTGGACAGCCAGCTCACCAAGGCCAATCAGGATCTGTTGCAGCAGGCACGCGAATTGCCGGCACC
GCTCGACGTCTGGGTGGCAGGCGTGGCGGCGGATGTCGGCTCGCTCGCGGTCAAGTCCGCACGCAGCCGCATCGCCGAAC
TCTGGACGGCCGATTCAGCCTCCCTGTGCTCCTCGATCGTGACCGGTCGTTATCCCTTCGACCGAGCCTCCTCCCGCGAT
GTCGCGATTGCGGATTTCACCCGTCTCTTCGCGCCGACGGGGGTTTTCCAGAGCTTCTTCAAACAGCGCATGGAACCGTT
TGTCGACAAGACGACGACCCCATGGAGCTGGAAAGGCACCTTCGGCGCCACCGGCATTCCCAGCAGCGCCGTCGCCCAGT
TCGAAAATGCCGACAAGATTTCGCGCGCCTTTTTCCCGAATGGCAGCGAGACGCCGGCGGTTTCGATCAATGTCAAACCC
GTCTCGCTCACCAATGCCTCCAGCGCGGTGATGCTGGAAATCGAAGGCGAGCGGGTGGTTTATTATCATGGACCGATCCA
GGCGAAATCGATTACCTGGCCCTCGCGCGAAAACACGGCGAGCCTGTCGCGCATCGCCTTCCAGCCGGGCGGCTGGCAGC
AGGCGAAGACCGAAAACGGCGACTGGTCGCCCTTCCGGCTGTTTGACGGTGCCAATATCGAAAATCAGTCGGGTGAGCTG
TTGCGGGTGCGGTTTGAGAATGGCGTCCAGGCTGCCGAGTTCGATATTCAGTTTGGCTCGGTTCTCAATCCATTCAAGCT
AGATGCGATTGCCAGCTTCGCCTGCCCTGCGCAGTTCTAG