SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015535
Component protein type	TssJ
T6SS ID (Type)	T6SS01236 (Type i5)
Strain	Vibrio vulnificus 106-2A
Replicon	chromosome
Sequence	Protein sequence (152 a.a.); Nucleotide sequence (456 bp)
Reference	[1] Church SR, Lux T, Baker-Austin C, et al. Vibrio vulnificus Type 6 Secretion System 1 Contains Anti-Bacterial Properties. PLoS One. 2016 Oct 31;11(10):e0165500. doi: 10.1371/journal.pone.0165500. eCollection 2016. PMID: 27798649

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VV1062A_RS02960
Coordinate (Strand)	453151..453606 (-)
NCBI ID	1119684964
RefSeq	NZ_LMTD01000002
Uniprot ID	UPI00092BEBD8
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.8e-34	31..145

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	MKDMVK
Transmembrane helix	7-29	IITTLIVFLLVGCSAANMVVSPY
Outside	30-151	SNLEIKTGFNVNPDMNGRPSPVVVYVLELTSDTLFTSQDFFSLYENTAQTLGPDLVNKTELYLSPGQKTLYESTMKPSVEYIGIVVAYRDIENANWRKVIKVDRKGYNTYELSLDELSLAIK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9884	pos: 18-19. LVG-CS.	MKDMVKIITTLIVFLLVG

Protein sequence: 152 a.a.

						>T6CP015535 NZ_LMTD01000002:c453606-453151 [Vibrio vulnificus] [TssJ]
MKDMVKIITTLIVFLLVGCSAANMVVSPYSNLEIKTGFNVNPDMNGRPSPVVVYVLELTSDTLFTSQDFFSLYENTAQTL
GPDLVNKTELYLSPGQKTLYESTMKPSVEYIGIVVAYRDIENANWRKVIKVDRKGYNTYELSLDELSLAIK*					

Nucleotide sequence: 456 bp

						>T6CP015535 NZ_LMTD01000002:c453606-453151 [Vibrio vulnificus] [TssJ]
ATGAAAGACATGGTAAAAATAATTACCACACTAATAGTGTTTTTATTAGTGGGTTGCAGTGCGGCAAATATGGTTGTTTC
TCCCTATTCAAATTTGGAAATAAAGACAGGGTTTAATGTCAATCCAGATATGAATGGCAGACCTTCACCTGTCGTAGTTT
ATGTCCTTGAACTCACTTCTGATACCTTATTTACGAGTCAAGACTTCTTCTCTCTATATGAAAATACAGCCCAAACGCTA
GGCCCGGATCTGGTGAACAAAACAGAATTGTATCTCTCCCCAGGCCAAAAAACACTTTATGAATCTACGATGAAACCAAG
TGTTGAATATATCGGCATTGTAGTGGCATATCGAGACATCGAAAATGCAAATTGGCGCAAGGTGATAAAGGTCGACCGCA
AGGGCTATAACACCTACGAGCTTTCACTAGATGAATTGTCTTTGGCTATAAAATAG					