Detailed information of component protein
Summary
Component ID | T6CP015515 |
Component protein type | TssM |
T6SS ID (Type) | T6SS01235 (Type i1) |
Strain | Vibrio vulnificus 106-2A |
Replicon | chromosome |
Sequence | Protein sequence (1167 a.a.); Nucleotide sequence (3501 bp) |
Reference |
External database links
Locus tag (Gene) | VV1062A_RS01775 |
Coordinate (Strand) | 174060..177560 (+) |
NCBI ID | 1867138634 |
RefSeq | NZ_LMTD01000002 |
Uniprot ID | - |
KEGG ID | - |
PDB ID | - |
Pfam domain hit(s)
Domain | Pfam ID | E-value | Aligned region |
---|---|---|---|
IcmF-related_N | PF14331.8 | 9.6e-77 | 188..445 |
IcmF-related | PF06761.14 | 3e-73 | 495..809 |
IcmF_C | PF06744.14 | 1.9e-23 | 1039..1145 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
Prediction | Region | Sequence |
---|---|---|
Inside | 1-4 | VVAA |
Transmembrane helix | 5-27 | LPILLFTTFILLNVAIWWAGPWL |
Outside | 28-36 | EFAGYKPLE |
Transmembrane helix | 37-59 | SIVARVVASSLFTLSCLAIWGIW |
Inside | 60-437 | QWRKLQTFRAEQKREDQLRQDPIKVYEERQEVELNQVMNNMKQNLNKHNYLYALPWYLVLGLENAGKTSLINRSGQNFVFSSVMRASGQKSENPYSFDWWIGDESVLIDPDGELLTQGNRSEDNNGAMERRLWLHFVDWLDRTRSRRPLNGIVLALDVSHLATATASERKAYASLLRARLRELMETLSTRLPVYIALTKLDLLHGFDPFFKHYTKSQREEVLGFTFSMDSLDNLDSWLDEFAEEYAQFVERMNRMLPHAVAMPMTLEERNAIYSFTRQLFGLKDILQQFFKEALESDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYSLSHAINTAQRAKNSTVYFTQKLFTHIIYPEAGLASDNFRVARNKRR |
Transmembrane helix | 438-457 | LIGLSFIACTIATLLLAGTW |
Outside | 458-1166 | HRSYINNVQHADAVLEKVNQYKEQFSSNRNLASQKDVLEPLNKIREATLEFGFFRDKPKYVSDFGLYQGHTIGPKVEETYLNLLENRFLPLLMADVVVALNQAQSDEEKLAVLRVYRMLVDKSGRYQDYVMDYFAKYWQMSFSGQRKTQEELLGHLDYAMRHTDLTADRLNDDKGAEKVMRPYDKVIAKAQVELSSMPNDQRVYRNLKLSAQTVLGPPVNLRSLIGPVFDVVFEERVLNSKNLYIPQMLTKRGFDDYFMPQSESVSELALIDSWVLGQSKTAQFSEADKQALRDKIRDLYVADYTNTWRSALNEIDVKYFNDINDAVMVLENITSNLEPMQRLLRTLEDNTQLYSALPKDEAAIKELLKNPKYKVASMIETPFAELNSMLQSADSKPAYMTEVLASVDELKSYLKSIQDAPDVGMAALDATKARVKLVNADPIYTLKRVSSGLPKPLDTMMTKIADESWYVVKQEAIKHLEVRWAEDVYDTFQSKLAGRYPFNSASNKDVSLEDFETFFAPNGTLDNFYNHQLKMFIEENITVSADDSTQSIVRKEVLEQIEQAQKIREAFFNRKGILDVSFSVEPLSLSNNKRRSVLNVDGQFLAYSHGQRENVELIWPNTLRDSAVSKVTLVPTQSNMSPRSIQIQGPWAFFRLLDEGDVVAASQTSVDFKFIVDGGEMIYRINSEADANPFTERLFKSFKLSKTLY |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction | Probability | Cleavage site | Signal peptide sequence |
---|---|---|---|
Other | 0.9973 | - | - |
Protein sequence: 1167 a.a.
>T6CP015515 NZ_LMTD01000002:174060-177560 [Vibrio vulnificus] [TssM]
VVAALPILLFTTFILLNVAIWWAGPWLEFAGYKPLESIVARVVASSLFTLSCLAIWGIWQWRKLQTFRAEQKREDQLRQD
PIKVYEERQEVELNQVMNNMKQNLNKHNYLYALPWYLVLGLENAGKTSLINRSGQNFVFSSVMRASGQKSENPYSFDWWI
GDESVLIDPDGELLTQGNRSEDNNGAMERRLWLHFVDWLDRTRSRRPLNGIVLALDVSHLATATASERKAYASLLRARLR
ELMETLSTRLPVYIALTKLDLLHGFDPFFKHYTKSQREEVLGFTFSMDSLDNLDSWLDEFAEEYAQFVERMNRMLPHAVA
MPMTLEERNAIYSFTRQLFGLKDILQQFFKEALESDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYSLSHAINTAQ
RAKNSTVYFTQKLFTHIIYPEAGLASDNFRVARNKRRLIGLSFIACTIATLLLAGTWHRSYINNVQHADAVLEKVNQYKE
QFSSNRNLASQKDVLEPLNKIREATLEFGFFRDKPKYVSDFGLYQGHTIGPKVEETYLNLLENRFLPLLMADVVVALNQA
QSDEEKLAVLRVYRMLVDKSGRYQDYVMDYFAKYWQMSFSGQRKTQEELLGHLDYAMRHTDLTADRLNDDKGAEKVMRPY
DKVIAKAQVELSSMPNDQRVYRNLKLSAQTVLGPPVNLRSLIGPVFDVVFEERVLNSKNLYIPQMLTKRGFDDYFMPQSE
SVSELALIDSWVLGQSKTAQFSEADKQALRDKIRDLYVADYTNTWRSALNEIDVKYFNDINDAVMVLENITSNLEPMQRL
LRTLEDNTQLYSALPKDEAAIKELLKNPKYKVASMIETPFAELNSMLQSADSKPAYMTEVLASVDELKSYLKSIQDAPDV
GMAALDATKARVKLVNADPIYTLKRVSSGLPKPLDTMMTKIADESWYVVKQEAIKHLEVRWAEDVYDTFQSKLAGRYPFN
SASNKDVSLEDFETFFAPNGTLDNFYNHQLKMFIEENITVSADDSTQSIVRKEVLEQIEQAQKIREAFFNRKGILDVSFS
VEPLSLSNNKRRSVLNVDGQFLAYSHGQRENVELIWPNTLRDSAVSKVTLVPTQSNMSPRSIQIQGPWAFFRLLDEGDVV
AASQTSVDFKFIVDGGEMIYRINSEADANPFTERLFKSFKLSKTLY*
VVAALPILLFTTFILLNVAIWWAGPWLEFAGYKPLESIVARVVASSLFTLSCLAIWGIWQWRKLQTFRAEQKREDQLRQD
PIKVYEERQEVELNQVMNNMKQNLNKHNYLYALPWYLVLGLENAGKTSLINRSGQNFVFSSVMRASGQKSENPYSFDWWI
GDESVLIDPDGELLTQGNRSEDNNGAMERRLWLHFVDWLDRTRSRRPLNGIVLALDVSHLATATASERKAYASLLRARLR
ELMETLSTRLPVYIALTKLDLLHGFDPFFKHYTKSQREEVLGFTFSMDSLDNLDSWLDEFAEEYAQFVERMNRMLPHAVA
MPMTLEERNAIYSFTRQLFGLKDILQQFFKEALESDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYSLSHAINTAQ
RAKNSTVYFTQKLFTHIIYPEAGLASDNFRVARNKRRLIGLSFIACTIATLLLAGTWHRSYINNVQHADAVLEKVNQYKE
QFSSNRNLASQKDVLEPLNKIREATLEFGFFRDKPKYVSDFGLYQGHTIGPKVEETYLNLLENRFLPLLMADVVVALNQA
QSDEEKLAVLRVYRMLVDKSGRYQDYVMDYFAKYWQMSFSGQRKTQEELLGHLDYAMRHTDLTADRLNDDKGAEKVMRPY
DKVIAKAQVELSSMPNDQRVYRNLKLSAQTVLGPPVNLRSLIGPVFDVVFEERVLNSKNLYIPQMLTKRGFDDYFMPQSE
SVSELALIDSWVLGQSKTAQFSEADKQALRDKIRDLYVADYTNTWRSALNEIDVKYFNDINDAVMVLENITSNLEPMQRL
LRTLEDNTQLYSALPKDEAAIKELLKNPKYKVASMIETPFAELNSMLQSADSKPAYMTEVLASVDELKSYLKSIQDAPDV
GMAALDATKARVKLVNADPIYTLKRVSSGLPKPLDTMMTKIADESWYVVKQEAIKHLEVRWAEDVYDTFQSKLAGRYPFN
SASNKDVSLEDFETFFAPNGTLDNFYNHQLKMFIEENITVSADDSTQSIVRKEVLEQIEQAQKIREAFFNRKGILDVSFS
VEPLSLSNNKRRSVLNVDGQFLAYSHGQRENVELIWPNTLRDSAVSKVTLVPTQSNMSPRSIQIQGPWAFFRLLDEGDVV
AASQTSVDFKFIVDGGEMIYRINSEADANPFTERLFKSFKLSKTLY*
Nucleotide sequence: 3501 bp
>T6CP015515 NZ_LMTD01000002:174060-177560 [Vibrio vulnificus] [TssM]
GTGGTCGCTGCATTACCAATTTTGCTATTCACTACGTTCATATTGTTGAACGTCGCCATTTGGTGGGCAGGCCCCTGGTT
AGAATTCGCCGGTTATAAACCTTTGGAGTCTATTGTGGCTCGAGTTGTGGCGAGTAGCTTATTTACTCTCAGTTGTCTAG
CCATTTGGGGCATTTGGCAATGGCGAAAACTGCAAACTTTTCGAGCGGAACAAAAACGTGAAGACCAGTTACGTCAAGAC
CCGATAAAAGTGTATGAAGAAAGACAAGAAGTTGAGTTGAATCAGGTCATGAACAACATGAAGCAGAACCTGAACAAACA
CAACTACTTATACGCGTTACCATGGTACTTAGTATTGGGGTTAGAAAACGCAGGAAAAACCAGCTTAATCAACCGTTCAG
GACAAAATTTTGTTTTTTCTTCAGTAATGCGAGCGTCGGGTCAAAAAAGTGAAAATCCGTATTCATTTGATTGGTGGATT
GGTGACGAATCTGTGTTAATTGACCCTGATGGTGAGTTATTAACCCAAGGTAATCGAAGTGAAGACAACAACGGGGCAAT
GGAACGTCGTTTATGGTTACATTTTGTAGATTGGCTTGACCGTACGCGGAGTAGGCGTCCCCTTAATGGAATAGTACTAG
CTCTCGATGTTTCCCACCTTGCAACGGCTACGGCATCCGAGCGTAAAGCTTATGCTAGTTTACTGCGTGCTCGTTTACGA
GAACTGATGGAGACACTATCGACTCGTTTACCAGTCTATATTGCTCTAACTAAACTCGATCTTCTTCATGGTTTTGACCC
CTTCTTTAAGCACTACACTAAAAGTCAGAGAGAAGAGGTGTTAGGTTTCACATTCTCAATGGATTCGTTAGATAACCTTG
ATTCATGGTTAGATGAATTTGCAGAGGAATATGCACAATTTGTTGAACGGATGAATCGAATGCTACCACATGCAGTAGCA
ATGCCGATGACGTTGGAAGAGCGAAACGCGATTTATAGTTTTACTCGGCAGCTATTTGGCCTAAAAGATATTCTTCAGCA
GTTTTTCAAAGAAGCGTTGGAGAGTGATCAGTTTTCTACTTCTGCCTTGGTGAGGGGAGCGTACTTTACTTCTGTTTATC
AGCAAGGCGTTCCTACAAATGCCTTTGATGATGCAGCTTCACGTCGTTATAGTCTCTCACACGCGATTAATACTGCGCAG
AGAGCGAAAAACTCAACCGTCTATTTCACACAGAAATTGTTTACGCACATAATTTATCCAGAAGCAGGTTTGGCTTCGGA
TAACTTTCGTGTTGCAAGAAATAAACGTCGTTTAATTGGACTTTCCTTCATTGCATGTACAATTGCAACATTACTTTTGG
CGGGAACTTGGCATCGTAGTTATATTAACAATGTGCAACATGCCGATGCAGTATTAGAAAAAGTAAACCAGTATAAAGAG
CAGTTTTCGAGCAATCGCAATCTTGCCTCTCAGAAAGACGTACTAGAACCGCTGAATAAGATTCGTGAAGCGACTCTAGA
ATTTGGTTTCTTTAGAGATAAGCCGAAATATGTTTCAGATTTTGGTCTTTACCAAGGGCATACCATTGGCCCTAAAGTTG
AAGAAACCTACCTCAATTTACTTGAAAACCGTTTTTTGCCCTTACTCATGGCCGATGTGGTAGTGGCTTTAAATCAAGCG
CAGAGTGATGAGGAGAAGCTTGCGGTCCTTCGTGTCTATCGAATGTTGGTAGATAAAAGTGGTCGATACCAAGATTACGT
GATGGATTATTTCGCTAAGTATTGGCAGATGTCTTTTTCGGGTCAACGCAAAACTCAAGAGGAATTACTAGGGCACTTGG
ATTATGCGATGCGTCATACCGATTTAACCGCAGATCGTCTAAACGATGATAAAGGGGCTGAAAAGGTTATGCGTCCATAC
GATAAAGTGATCGCGAAAGCCCAGGTTGAGTTGAGTTCAATGCCAAACGATCAGCGTGTCTACAGAAACCTTAAGCTGAG
TGCGCAAACGGTTCTAGGTCCTCCAGTGAATCTTAGAAGCTTGATTGGTCCGGTTTTTGATGTCGTTTTTGAAGAACGGG
TACTGAATAGCAAGAATTTGTATATTCCTCAAATGTTAACCAAACGTGGGTTTGATGACTATTTTATGCCGCAGTCTGAA
TCAGTTTCCGAGTTGGCTTTGATTGATAGCTGGGTATTGGGTCAAAGTAAAACGGCACAATTTAGTGAAGCTGACAAACA
GGCACTGCGCGACAAAATACGAGATCTCTACGTCGCTGATTATACAAACACATGGCGCTCAGCACTGAACGAGATAGATG
TTAAGTATTTTAATGATATCAATGATGCAGTTATGGTGTTAGAGAACATCACGAGTAATCTCGAACCAATGCAACGCTTA
CTTCGTACTCTAGAGGATAATACGCAACTGTATAGCGCGTTACCTAAAGACGAAGCTGCAATCAAAGAATTGCTGAAAAA
TCCAAAGTACAAAGTGGCTTCTATGATAGAAACACCATTTGCTGAACTTAACAGTATGCTCCAGTCTGCTGACAGTAAGC
CAGCATACATGACAGAAGTGCTCGCATCGGTTGATGAGTTAAAGAGTTATCTAAAATCGATCCAAGATGCGCCGGATGTT
GGCATGGCTGCATTAGATGCGACCAAAGCGCGAGTAAAACTAGTCAACGCGGATCCTATTTATACATTAAAGCGCGTCTC
ATCTGGGTTACCTAAACCACTGGATACAATGATGACGAAAATTGCAGATGAGAGCTGGTATGTTGTTAAACAAGAAGCGA
TCAAGCATCTTGAAGTGCGCTGGGCTGAAGACGTTTATGACACGTTCCAAAGTAAATTAGCAGGACGTTATCCATTTAAT
TCTGCTTCCAATAAAGATGTTTCCTTGGAAGATTTTGAAACTTTCTTTGCGCCTAATGGTACGCTGGATAACTTTTATAA
TCATCAGTTAAAGATGTTTATTGAAGAGAATATAACTGTTTCAGCAGATGACTCTACGCAATCAATTGTCCGTAAAGAGG
TACTAGAGCAGATTGAACAAGCACAAAAAATTCGAGAAGCATTCTTTAATCGTAAAGGTATCTTAGATGTTAGTTTCTCC
GTCGAACCGCTTAGTCTGAGTAACAACAAACGGCGGAGTGTATTGAATGTCGACGGTCAGTTTTTGGCTTATAGCCATGG
ACAGCGTGAAAATGTTGAATTGATTTGGCCAAATACTCTACGTGATTCCGCTGTTTCTAAAGTCACGTTGGTACCGACTC
AAAGCAACATGTCGCCACGCAGTATACAGATACAAGGGCCTTGGGCATTCTTTCGATTGTTAGATGAAGGAGATGTGGTT
GCGGCTAGTCAGACGTCAGTAGATTTTAAGTTCATAGTTGATGGTGGTGAAATGATCTACAGAATTAACTCAGAAGCGGA
TGCAAATCCTTTTACTGAACGTCTTTTCAAGTCATTCAAACTGTCTAAAACTCTTTATTAA
GTGGTCGCTGCATTACCAATTTTGCTATTCACTACGTTCATATTGTTGAACGTCGCCATTTGGTGGGCAGGCCCCTGGTT
AGAATTCGCCGGTTATAAACCTTTGGAGTCTATTGTGGCTCGAGTTGTGGCGAGTAGCTTATTTACTCTCAGTTGTCTAG
CCATTTGGGGCATTTGGCAATGGCGAAAACTGCAAACTTTTCGAGCGGAACAAAAACGTGAAGACCAGTTACGTCAAGAC
CCGATAAAAGTGTATGAAGAAAGACAAGAAGTTGAGTTGAATCAGGTCATGAACAACATGAAGCAGAACCTGAACAAACA
CAACTACTTATACGCGTTACCATGGTACTTAGTATTGGGGTTAGAAAACGCAGGAAAAACCAGCTTAATCAACCGTTCAG
GACAAAATTTTGTTTTTTCTTCAGTAATGCGAGCGTCGGGTCAAAAAAGTGAAAATCCGTATTCATTTGATTGGTGGATT
GGTGACGAATCTGTGTTAATTGACCCTGATGGTGAGTTATTAACCCAAGGTAATCGAAGTGAAGACAACAACGGGGCAAT
GGAACGTCGTTTATGGTTACATTTTGTAGATTGGCTTGACCGTACGCGGAGTAGGCGTCCCCTTAATGGAATAGTACTAG
CTCTCGATGTTTCCCACCTTGCAACGGCTACGGCATCCGAGCGTAAAGCTTATGCTAGTTTACTGCGTGCTCGTTTACGA
GAACTGATGGAGACACTATCGACTCGTTTACCAGTCTATATTGCTCTAACTAAACTCGATCTTCTTCATGGTTTTGACCC
CTTCTTTAAGCACTACACTAAAAGTCAGAGAGAAGAGGTGTTAGGTTTCACATTCTCAATGGATTCGTTAGATAACCTTG
ATTCATGGTTAGATGAATTTGCAGAGGAATATGCACAATTTGTTGAACGGATGAATCGAATGCTACCACATGCAGTAGCA
ATGCCGATGACGTTGGAAGAGCGAAACGCGATTTATAGTTTTACTCGGCAGCTATTTGGCCTAAAAGATATTCTTCAGCA
GTTTTTCAAAGAAGCGTTGGAGAGTGATCAGTTTTCTACTTCTGCCTTGGTGAGGGGAGCGTACTTTACTTCTGTTTATC
AGCAAGGCGTTCCTACAAATGCCTTTGATGATGCAGCTTCACGTCGTTATAGTCTCTCACACGCGATTAATACTGCGCAG
AGAGCGAAAAACTCAACCGTCTATTTCACACAGAAATTGTTTACGCACATAATTTATCCAGAAGCAGGTTTGGCTTCGGA
TAACTTTCGTGTTGCAAGAAATAAACGTCGTTTAATTGGACTTTCCTTCATTGCATGTACAATTGCAACATTACTTTTGG
CGGGAACTTGGCATCGTAGTTATATTAACAATGTGCAACATGCCGATGCAGTATTAGAAAAAGTAAACCAGTATAAAGAG
CAGTTTTCGAGCAATCGCAATCTTGCCTCTCAGAAAGACGTACTAGAACCGCTGAATAAGATTCGTGAAGCGACTCTAGA
ATTTGGTTTCTTTAGAGATAAGCCGAAATATGTTTCAGATTTTGGTCTTTACCAAGGGCATACCATTGGCCCTAAAGTTG
AAGAAACCTACCTCAATTTACTTGAAAACCGTTTTTTGCCCTTACTCATGGCCGATGTGGTAGTGGCTTTAAATCAAGCG
CAGAGTGATGAGGAGAAGCTTGCGGTCCTTCGTGTCTATCGAATGTTGGTAGATAAAAGTGGTCGATACCAAGATTACGT
GATGGATTATTTCGCTAAGTATTGGCAGATGTCTTTTTCGGGTCAACGCAAAACTCAAGAGGAATTACTAGGGCACTTGG
ATTATGCGATGCGTCATACCGATTTAACCGCAGATCGTCTAAACGATGATAAAGGGGCTGAAAAGGTTATGCGTCCATAC
GATAAAGTGATCGCGAAAGCCCAGGTTGAGTTGAGTTCAATGCCAAACGATCAGCGTGTCTACAGAAACCTTAAGCTGAG
TGCGCAAACGGTTCTAGGTCCTCCAGTGAATCTTAGAAGCTTGATTGGTCCGGTTTTTGATGTCGTTTTTGAAGAACGGG
TACTGAATAGCAAGAATTTGTATATTCCTCAAATGTTAACCAAACGTGGGTTTGATGACTATTTTATGCCGCAGTCTGAA
TCAGTTTCCGAGTTGGCTTTGATTGATAGCTGGGTATTGGGTCAAAGTAAAACGGCACAATTTAGTGAAGCTGACAAACA
GGCACTGCGCGACAAAATACGAGATCTCTACGTCGCTGATTATACAAACACATGGCGCTCAGCACTGAACGAGATAGATG
TTAAGTATTTTAATGATATCAATGATGCAGTTATGGTGTTAGAGAACATCACGAGTAATCTCGAACCAATGCAACGCTTA
CTTCGTACTCTAGAGGATAATACGCAACTGTATAGCGCGTTACCTAAAGACGAAGCTGCAATCAAAGAATTGCTGAAAAA
TCCAAAGTACAAAGTGGCTTCTATGATAGAAACACCATTTGCTGAACTTAACAGTATGCTCCAGTCTGCTGACAGTAAGC
CAGCATACATGACAGAAGTGCTCGCATCGGTTGATGAGTTAAAGAGTTATCTAAAATCGATCCAAGATGCGCCGGATGTT
GGCATGGCTGCATTAGATGCGACCAAAGCGCGAGTAAAACTAGTCAACGCGGATCCTATTTATACATTAAAGCGCGTCTC
ATCTGGGTTACCTAAACCACTGGATACAATGATGACGAAAATTGCAGATGAGAGCTGGTATGTTGTTAAACAAGAAGCGA
TCAAGCATCTTGAAGTGCGCTGGGCTGAAGACGTTTATGACACGTTCCAAAGTAAATTAGCAGGACGTTATCCATTTAAT
TCTGCTTCCAATAAAGATGTTTCCTTGGAAGATTTTGAAACTTTCTTTGCGCCTAATGGTACGCTGGATAACTTTTATAA
TCATCAGTTAAAGATGTTTATTGAAGAGAATATAACTGTTTCAGCAGATGACTCTACGCAATCAATTGTCCGTAAAGAGG
TACTAGAGCAGATTGAACAAGCACAAAAAATTCGAGAAGCATTCTTTAATCGTAAAGGTATCTTAGATGTTAGTTTCTCC
GTCGAACCGCTTAGTCTGAGTAACAACAAACGGCGGAGTGTATTGAATGTCGACGGTCAGTTTTTGGCTTATAGCCATGG
ACAGCGTGAAAATGTTGAATTGATTTGGCCAAATACTCTACGTGATTCCGCTGTTTCTAAAGTCACGTTGGTACCGACTC
AAAGCAACATGTCGCCACGCAGTATACAGATACAAGGGCCTTGGGCATTCTTTCGATTGTTAGATGAAGGAGATGTGGTT
GCGGCTAGTCAGACGTCAGTAGATTTTAAGTTCATAGTTGATGGTGGTGAAATGATCTACAGAATTAACTCAGAAGCGGA
TGCAAATCCTTTTACTGAACGTCTTTTCAAGTCATTCAAACTGTCTAAAACTCTTTATTAA