Detailed information of component protein
Summary
| Component ID | T6CP015513 |
| Component protein type | VasI |
| T6SS ID (Type) | T6SS01235  (Type i1) |
| Strain | Vibrio vulnificus 106-2A |
| Replicon | chromosome |
| Sequence | Protein sequence (227 a.a.); Nucleotide sequence (681 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | VV1062A_RS01765 |
| Coordinate (Strand) | 171906..172586 (+) |
| NCBI ID | 1337739952 |
| RefSeq | NZ_LMTD01000002 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| VasI | PF11319.10 | 8.1e-50 | 44..226 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-226 | MPEIGDQSSMNKHALTVSVLLGVSTSVSLVWAQSSPSILEQAEQCRNITTRLERLACFDRAFSISPEKMIILPTNSYPREWKRAMDAIELTSYQRGWILENTGDGRGSSAWIALPAQNVPTDDQGRPVLLLSCINNLSRIELALPQAVVDARIQISVHQKTQFWRSDDTGVLFSSARGMPAIEMMKLVASESRLLLRSNASFADGLQFDTQGLNEALNALRERCGW |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.7322 | pos: 32-33. VWA-QS. | MPEIGDQSSMNKHALTVSVLLGVSTSVSLVWA |
Protein sequence: 227 a.a.
>T6CP015513 NZ_LMTD01000002:171906-172586 [Vibrio vulnificus] [VasI]
MPEIGDQSSMNKHALTVSVLLGVSTSVSLVWAQSSPSILEQAEQCRNITTRLERLACFDRAFSISPEKMIILPTNSYPRE
WKRAMDAIELTSYQRGWILENTGDGRGSSAWIALPAQNVPTDDQGRPVLLLSCINNLSRIELALPQAVVDARIQISVHQK
TQFWRSDDTGVLFSSARGMPAIEMMKLVASESRLLLRSNASFADGLQFDTQGLNEALNALRERCGW*
MPEIGDQSSMNKHALTVSVLLGVSTSVSLVWAQSSPSILEQAEQCRNITTRLERLACFDRAFSISPEKMIILPTNSYPRE
WKRAMDAIELTSYQRGWILENTGDGRGSSAWIALPAQNVPTDDQGRPVLLLSCINNLSRIELALPQAVVDARIQISVHQK
TQFWRSDDTGVLFSSARGMPAIEMMKLVASESRLLLRSNASFADGLQFDTQGLNEALNALRERCGW*
Nucleotide sequence: 681 bp
>T6CP015513 NZ_LMTD01000002:171906-172586 [Vibrio vulnificus] [VasI]
ATGCCTGAAATTGGAGATCAAAGCTCCATGAATAAACATGCACTAACAGTTAGTGTGCTCTTGGGTGTCTCAACTTCGGT
TAGTTTAGTTTGGGCGCAGAGTTCGCCCTCAATACTTGAACAAGCTGAGCAGTGTAGAAATATCACCACTCGCTTAGAGC
GCTTAGCTTGCTTTGACCGCGCATTCTCTATCTCTCCTGAAAAAATGATTATCTTGCCAACTAACTCATACCCTCGTGAG
TGGAAAAGAGCGATGGATGCCATTGAGCTTACCTCTTATCAACGTGGTTGGATATTGGAAAACACGGGAGATGGCCGAGG
AAGTAGTGCTTGGATTGCGCTACCTGCTCAAAATGTTCCAACTGATGATCAAGGGCGTCCTGTGCTGTTACTTAGTTGTA
TTAACAACCTTAGTCGAATAGAACTCGCGCTTCCACAAGCAGTCGTGGATGCACGAATTCAAATCTCAGTTCACCAAAAA
ACTCAATTTTGGCGTAGTGATGATACCGGGGTGTTGTTTTCTTCCGCACGAGGTATGCCAGCAATAGAAATGATGAAATT
GGTGGCAAGTGAGTCCCGTTTGCTGTTGCGCTCAAATGCCTCGTTTGCAGATGGACTTCAGTTTGATACTCAAGGTCTAA
ATGAAGCCCTTAACGCATTACGTGAACGTTGCGGTTGGTAA
ATGCCTGAAATTGGAGATCAAAGCTCCATGAATAAACATGCACTAACAGTTAGTGTGCTCTTGGGTGTCTCAACTTCGGT
TAGTTTAGTTTGGGCGCAGAGTTCGCCCTCAATACTTGAACAAGCTGAGCAGTGTAGAAATATCACCACTCGCTTAGAGC
GCTTAGCTTGCTTTGACCGCGCATTCTCTATCTCTCCTGAAAAAATGATTATCTTGCCAACTAACTCATACCCTCGTGAG
TGGAAAAGAGCGATGGATGCCATTGAGCTTACCTCTTATCAACGTGGTTGGATATTGGAAAACACGGGAGATGGCCGAGG
AAGTAGTGCTTGGATTGCGCTACCTGCTCAAAATGTTCCAACTGATGATCAAGGGCGTCCTGTGCTGTTACTTAGTTGTA
TTAACAACCTTAGTCGAATAGAACTCGCGCTTCCACAAGCAGTCGTGGATGCACGAATTCAAATCTCAGTTCACCAAAAA
ACTCAATTTTGGCGTAGTGATGATACCGGGGTGTTGTTTTCTTCCGCACGAGGTATGCCAGCAATAGAAATGATGAAATT
GGTGGCAAGTGAGTCCCGTTTGCTGTTGCGCTCAAATGCCTCGTTTGCAGATGGACTTCAGTTTGATACTCAAGGTCTAA
ATGAAGCCCTTAACGCATTACGTGAACGTTGCGGTTGGTAA