Detailed information of component protein
Summary
| Component ID | T6CP015510 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS01235  (Type i1) |
| Strain | Vibrio vulnificus 106-2A |
| Replicon | chromosome |
| Sequence | Protein sequence (258 a.a.); Nucleotide sequence (774 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | VV1062A_RS01750 |
| Coordinate (Strand) | 166924..167697 (+) |
| NCBI ID | 1119685170 |
| RefSeq | NZ_LMTD01000002 |
| Uniprot ID | A0A2S3R394_VIBVL |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 8.7e-60 | 39..240 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-219 | MQQNKKETPLSNLLFDDVEKINHDQDYWFQLRGDNRNLLIDAATPLFGFSLRVRSLTECDNIDQIYRQTIEEIKAIEIELTEQGYDHSILMAYRYILCAFLDESVMGTEWGASSVWAEHSMLSRFHNETWGGEKVFTILNRLEGEPERYQTLIAFIYHCLVLGFEGKYRVMEGGKAEREKVISRVYRLINDLEESEPQDLTCPTEHVVRSKYTLSRQMP |
| Transmembrane helix | 220-242 | VWSVFIGFIVLWIGIFLGYSYML |
| Outside | 243-257 | HSKSSDVLGQLNQIL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9862 | - | - |
Protein sequence: 258 a.a.
>T6CP015510 NZ_LMTD01000002:166924-167697 [Vibrio vulnificus] [TssL]
MQQNKKETPLSNLLFDDVEKINHDQDYWFQLRGDNRNLLIDAATPLFGFSLRVRSLTECDNIDQIYRQTIEEIKAIEIEL
TEQGYDHSILMAYRYILCAFLDESVMGTEWGASSVWAEHSMLSRFHNETWGGEKVFTILNRLEGEPERYQTLIAFIYHCL
VLGFEGKYRVMEGGKAEREKVISRVYRLINDLEESEPQDLTCPTEHVVRSKYTLSRQMPVWSVFIGFIVLWIGIFLGYSY
MLHSKSSDVLGQLNQIL*
MQQNKKETPLSNLLFDDVEKINHDQDYWFQLRGDNRNLLIDAATPLFGFSLRVRSLTECDNIDQIYRQTIEEIKAIEIEL
TEQGYDHSILMAYRYILCAFLDESVMGTEWGASSVWAEHSMLSRFHNETWGGEKVFTILNRLEGEPERYQTLIAFIYHCL
VLGFEGKYRVMEGGKAEREKVISRVYRLINDLEESEPQDLTCPTEHVVRSKYTLSRQMPVWSVFIGFIVLWIGIFLGYSY
MLHSKSSDVLGQLNQIL*
Nucleotide sequence: 774 bp
>T6CP015510 NZ_LMTD01000002:166924-167697 [Vibrio vulnificus] [TssL]
ATGCAACAAAATAAAAAAGAGACACCATTATCCAATTTGCTGTTTGATGATGTAGAAAAAATTAACCACGACCAAGACTA
TTGGTTTCAACTCCGTGGTGATAACCGTAACTTACTCATTGATGCGGCAACACCTTTGTTTGGTTTTTCGCTACGTGTAC
GCTCTCTTACAGAATGCGACAACATCGATCAAATTTATCGACAAACAATAGAAGAAATAAAAGCAATTGAAATCGAGCTA
ACAGAGCAAGGTTATGATCATTCAATTTTGATGGCATATCGCTACATTCTCTGTGCGTTTTTAGATGAGTCCGTTATGGG
AACTGAGTGGGGGGCCTCTAGTGTCTGGGCTGAACATTCAATGCTTTCACGTTTTCACAATGAAACTTGGGGGGGGGAGA
AGGTATTCACAATCTTAAATAGATTGGAAGGTGAACCTGAGCGCTATCAAACTTTAATCGCCTTTATTTACCACTGCTTA
GTATTAGGCTTTGAAGGTAAATATCGCGTAATGGAGGGCGGAAAAGCTGAACGGGAGAAAGTCATTAGCCGTGTATACCG
ACTCATCAACGATTTAGAGGAAAGTGAGCCACAAGACTTAACTTGTCCGACAGAGCATGTTGTGCGTTCTAAATATACAT
TAAGCAGACAAATGCCTGTTTGGTCGGTGTTTATAGGCTTTATCGTTCTTTGGATAGGCATTTTTCTTGGCTATAGCTAC
ATGTTGCACAGCAAGTCCAGTGATGTGCTAGGTCAACTTAATCAAATACTTTAA
ATGCAACAAAATAAAAAAGAGACACCATTATCCAATTTGCTGTTTGATGATGTAGAAAAAATTAACCACGACCAAGACTA
TTGGTTTCAACTCCGTGGTGATAACCGTAACTTACTCATTGATGCGGCAACACCTTTGTTTGGTTTTTCGCTACGTGTAC
GCTCTCTTACAGAATGCGACAACATCGATCAAATTTATCGACAAACAATAGAAGAAATAAAAGCAATTGAAATCGAGCTA
ACAGAGCAAGGTTATGATCATTCAATTTTGATGGCATATCGCTACATTCTCTGTGCGTTTTTAGATGAGTCCGTTATGGG
AACTGAGTGGGGGGCCTCTAGTGTCTGGGCTGAACATTCAATGCTTTCACGTTTTCACAATGAAACTTGGGGGGGGGAGA
AGGTATTCACAATCTTAAATAGATTGGAAGGTGAACCTGAGCGCTATCAAACTTTAATCGCCTTTATTTACCACTGCTTA
GTATTAGGCTTTGAAGGTAAATATCGCGTAATGGAGGGCGGAAAAGCTGAACGGGAGAAAGTCATTAGCCGTGTATACCG
ACTCATCAACGATTTAGAGGAAAGTGAGCCACAAGACTTAACTTGTCCGACAGAGCATGTTGTGCGTTCTAAATATACAT
TAAGCAGACAAATGCCTGTTTGGTCGGTGTTTATAGGCTTTATCGTTCTTTGGATAGGCATTTTTCTTGGCTATAGCTAC
ATGTTGCACAGCAAGTCCAGTGATGTGCTAGGTCAACTTAATCAAATACTTTAA