Detailed information of component protein


Summary

Component ID T6CP015497
Component protein type TssA
T6SS ID (Type) T6SS01224 experimental (Type i1)
Strain Vibrio alginolyticus 12G01
Replicon chromosome
Sequence Protein sequence (525 a.a.); Nucleotide sequence (1575 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) V12G01_RS25475
Coordinate (Strand) 117821..119395 (-)
NCBI ID 491515750
RefSeq NZ_CH902589
Uniprot ID A0A1W6V7M1_VIBAL
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasJ PF16989.7 2.3e-70 247..497
ImpA_N PF06812.14 7e-27 10..120

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-524 MSNIDIDRLLNPISDESPVGNDARYEFCYEMMESEVKKFGSLFGETVDWSVVKTHATEVLEHHSKDLKAICYLVRALTEEFGLQGFEQGLKLLSEALSRFGVELYPSRKRGRDGAVEWLNHQFKLVSSKLAESAQSWDLVSNCISTIEEVQRQYDEVYQDSEADFFEIRTQLNVLSQQAAADEQSDDAVVNVEQAAPAPATQSAAEETPAQNEVQTAPVKAVPPAPKPAPVKKAVAKEVDVDTDFSSPTASKRTLKKVAEVMLHANPSDPLAYRIYRHLTWDDIDGLPDHQNNQTPLSLAVSSDQQAEYRDKAEQESDIDTIKRLERTLTDAPFWLTGHYFVYSMLNNLGFDDAALAVKQEVTRFVESLEGIEHLSFKNSIPFADEATLSWLSTQDTTSSTSQPVVQTVVISQEDSLPMEDITLENLGECAAELARKLELDSSGRGQFMLYLQLIAAYQSVGLYPLCLPYLEKGWEVQKAFNLASWEPHLSSQLEDLIRKTLHRLFGSKDLLPEKYEEWKAIYD



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9962 - -



  Protein sequence: 525 a.a.    

>T6CP015497 NZ_CH902589:c119395-117821 [Vibrio alginolyticus 12G01] [TssA]
MSNIDIDRLLNPISDESPVGNDARYEFCYEMMESEVKKFGSLFGETVDWSVVKTHATEVLEHHSKDLKAICYLVRALTEE
FGLQGFEQGLKLLSEALSRFGVELYPSRKRGRDGAVEWLNHQFKLVSSKLAESAQSWDLVSNCISTIEEVQRQYDEVYQD
SEADFFEIRTQLNVLSQQAAADEQSDDAVVNVEQAAPAPATQSAAEETPAQNEVQTAPVKAVPPAPKPAPVKKAVAKEVD
VDTDFSSPTASKRTLKKVAEVMLHANPSDPLAYRIYRHLTWDDIDGLPDHQNNQTPLSLAVSSDQQAEYRDKAEQESDID
TIKRLERTLTDAPFWLTGHYFVYSMLNNLGFDDAALAVKQEVTRFVESLEGIEHLSFKNSIPFADEATLSWLSTQDTTSS
TSQPVVQTVVISQEDSLPMEDITLENLGECAAELARKLELDSSGRGQFMLYLQLIAAYQSVGLYPLCLPYLEKGWEVQKA
FNLASWEPHLSSQLEDLIRKTLHRLFGSKDLLPEKYEEWKAIYD*

  Nucleotide sequence: 1575 bp    

>T6CP015497 NZ_CH902589:c119395-117821 [Vibrio alginolyticus 12G01] [TssA]
TTGTCCAACATTGATATCGATCGTCTGCTTAATCCTATTTCTGATGAATCTCCAGTGGGTAACGATGCCCGTTACGAGTT
TTGCTATGAAATGATGGAATCCGAAGTGAAGAAATTCGGTTCCTTGTTTGGCGAAACAGTAGATTGGAGCGTGGTAAAAA
CGCACGCGACTGAAGTTCTAGAGCACCACAGTAAAGACTTAAAAGCCATTTGTTACCTCGTTCGCGCGCTAACAGAAGAG
TTTGGTCTGCAAGGCTTTGAACAAGGTCTGAAGCTGCTTAGTGAAGCGTTAAGTCGCTTTGGCGTTGAATTGTATCCTTC
CCGAAAGCGGGGTCGAGATGGCGCGGTCGAATGGTTGAACCATCAGTTTAAACTGGTCAGCAGCAAGTTAGCGGAAAGTG
CTCAGTCATGGGATTTGGTATCAAACTGCATTTCGACTATTGAAGAAGTGCAGCGTCAGTACGACGAGGTTTATCAAGAC
TCAGAAGCGGATTTCTTTGAGATTCGCACTCAACTCAATGTTTTATCTCAACAAGCGGCGGCTGATGAGCAGAGCGATGA
TGCAGTCGTTAACGTTGAACAAGCGGCGCCTGCACCAGCTACGCAGTCTGCTGCCGAAGAAACGCCAGCGCAGAATGAAG
TGCAAACCGCACCGGTGAAGGCGGTTCCGCCGGCCCCTAAACCTGCGCCAGTTAAGAAAGCGGTAGCAAAAGAAGTCGAT
GTCGATACGGACTTCTCTTCGCCTACGGCTTCTAAGCGCACCTTGAAGAAGGTTGCGGAAGTCATGCTTCATGCGAACCC
AAGTGATCCGCTGGCTTACCGGATTTATCGTCATCTGACTTGGGATGACATTGATGGTCTACCCGACCACCAAAATAATC
AAACGCCTTTGAGCTTGGCAGTGTCGTCCGATCAACAAGCGGAATACCGAGATAAAGCTGAACAAGAAAGCGACATCGAC
ACCATTAAGCGTTTGGAGCGCACATTAACGGATGCGCCGTTTTGGCTGACGGGCCATTACTTCGTGTATTCGATGCTTAA
TAACTTGGGCTTTGATGATGCAGCGCTTGCTGTGAAACAAGAAGTGACGCGTTTTGTTGAATCGCTAGAGGGCATCGAGC
ACCTCTCATTTAAGAATTCTATACCATTTGCAGATGAAGCGACCTTGAGCTGGTTATCGACTCAAGATACTACTTCTTCG
ACTTCTCAACCCGTTGTTCAAACGGTTGTGATTTCGCAGGAAGACTCGCTGCCAATGGAGGATATCACTCTAGAAAATCT
AGGGGAATGTGCCGCAGAGCTTGCCCGCAAACTTGAGCTGGATAGCTCGGGACGTGGGCAGTTCATGCTGTATTTGCAAT
TGATAGCAGCTTACCAGTCTGTTGGATTATACCCTTTGTGTTTGCCGTATCTTGAGAAGGGGTGGGAAGTACAAAAAGCG
TTTAATCTCGCGAGCTGGGAACCCCACTTATCGTCACAGTTAGAAGACCTAATTCGAAAAACACTCCATCGGTTATTTGG
AAGCAAGGATCTTCTACCTGAGAAGTATGAAGAGTGGAAAGCAATTTACGACTAA