SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015496
Component protein type	TssB
T6SS ID (Type)	T6SS01224 (Type i1)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (169 a.a.); Nucleotide sequence (507 bp)
Reference	[1] Salomon D, Kinch LN, Trudgian DC, et al. Marker for type VI secretion system effectors. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6. doi: 10.1073/pnas.1406110111. Epub 2014 Jun 9. PMID: 24927539 [2] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	V12G01_RS25470
Coordinate (Strand)	117288..117794 (-)
NCBI ID	WP_005373381.1
RefSeq	NZ_CH902589
Uniprot ID	A0A4P8G4C5_9VIBR, A0A2I3CQF3_VIBAX, A0A7Y3VML6_9VIBR, A0A0L8C9C3_VIBAL, D0WYE3_VIBAL
KEGG ID	vag:N646_3986, vnl:D3H41_19830
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	6.9e-59	6..157

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-168	MSRDGSVAPKERINIRYVPATGDTQEDVELPLSMMVVGDFTARADETPIEERTPINIDKDNFNEVLEGMSPNVKVNVENRLSDEEGGQIGVDLTFQNMKDFSPEAIAKSVPELNSLLELREALVALKGPLGNVPAFRKKIASVLQDEEARKKLLDELSIGNDQDAKEE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9841	-	-

Protein sequence: 169 a.a.

						>T6CP015496 NZ_CH902589:c117794-117288 [Vibrio alginolyticus 12G01] [TssB]
MSRDGSVAPKERINIRYVPATGDTQEDVELPLSMMVVGDFTARADETPIEERTPINIDKDNFNEVLEGMSPNVKVNVENR
LSDEEGGQIGVDLTFQNMKDFSPEAIAKSVPELNSLLELREALVALKGPLGNVPAFRKKIASVLQDEEARKKLLDELSIG
NDQDAKEE*					

Nucleotide sequence: 507 bp

						>T6CP015496 NZ_CH902589:c117794-117288 [Vibrio alginolyticus 12G01] [TssB]
ATGTCACGTGACGGCTCGGTGGCTCCTAAAGAGCGAATTAATATCCGTTATGTTCCAGCAACTGGCGATACGCAAGAAGA
CGTAGAGCTGCCGCTAAGCATGATGGTAGTTGGCGACTTTACTGCGCGTGCAGATGAGACACCAATTGAAGAACGTACTC
CAATTAACATCGACAAAGACAACTTTAACGAAGTGCTAGAGGGCATGTCTCCAAACGTTAAAGTCAACGTTGAAAACCGT
CTTTCTGATGAAGAAGGCGGCCAGATTGGTGTTGATCTGACGTTCCAAAATATGAAGGACTTCTCTCCTGAAGCGATCGC
GAAAAGTGTACCTGAACTAAACAGCCTACTTGAGCTTCGCGAAGCCTTGGTTGCACTGAAAGGTCCTCTAGGAAATGTTC
CTGCGTTCCGTAAGAAGATCGCATCGGTTCTTCAAGATGAAGAAGCGAGAAAGAAACTGTTGGATGAACTAAGCATTGGC
AACGACCAAGACGCGAAAGAAGAGTAA					