SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015494
Component protein type	TssE
T6SS ID (Type)	T6SS01224 (Type i1)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (137 a.a.); Nucleotide sequence (411 bp)
Reference	[1] Salomon D, Kinch LN, Trudgian DC, et al. Marker for type VI secretion system effectors. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6. doi: 10.1073/pnas.1406110111. Epub 2014 Jun 9. PMID: 24927539 [2] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	V12G01_RS25460
Coordinate (Strand)	115332..115742 (-)
NCBI ID	491515743
RefSeq	NZ_CH902589
Uniprot ID	A0A2I3CPM4_VIBAX, C0LZM9_VIBAL
KEGG ID	vag:N646_3988
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	5.7e-18	29..115

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-136	MEKGYRLLERIELGEPKNCYEKVVSHKHLIESIHSHLADLLNTHSGNAMIDNEYGLPDFNDVLSNNTNLVRHIQKNITSTIERFEPRLLNVEVHYREDHHNPLQLGFGIRGEVSHNGGKVPMSIDVYMGTDGQFNV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9945	-	-

Protein sequence: 137 a.a.

						>T6CP015494 NZ_CH902589:c115742-115332 [Vibrio alginolyticus 12G01] [TssE]
MEKGYRLLERIELGEPKNCYEKVVSHKHLIESIHSHLADLLNTHSGNAMIDNEYGLPDFNDVLSNNTNLVRHIQKNITST
IERFEPRLLNVEVHYREDHHNPLQLGFGIRGEVSHNGGKVPMSIDVYMGTDGQFNV*					

Nucleotide sequence: 411 bp

						>T6CP015494 NZ_CH902589:c115742-115332 [Vibrio alginolyticus 12G01] [TssE]
ATGGAAAAAGGTTACCGTCTTTTAGAGCGGATCGAGCTAGGAGAACCAAAAAACTGCTACGAGAAAGTGGTGTCTCATAA
GCATTTGATTGAATCCATTCACTCGCATCTCGCTGACTTGTTGAATACGCACTCCGGCAACGCAATGATCGACAATGAGT
ATGGTCTGCCAGATTTTAACGACGTACTTTCTAACAACACAAACCTCGTTCGCCATATTCAAAAAAACATCACGTCAACC
ATAGAACGCTTTGAGCCCCGACTGCTGAATGTCGAGGTTCATTACCGTGAAGACCACCATAATCCATTACAACTCGGCTT
TGGTATTCGCGGCGAAGTTTCCCATAACGGCGGTAAAGTCCCAATGTCGATCGATGTCTACATGGGAACCGACGGCCAAT
TTAACGTTTAG					