SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015491
Component protein type	TssM
T6SS ID (Type)	T6SS01224 (Type i1)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (1130 a.a.); Nucleotide sequence (3390 bp)
Reference	[1] Salomon D, Kinch LN, Trudgian DC, et al. Marker for type VI secretion system effectors. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6. doi: 10.1073/pnas.1406110111. Epub 2014 Jun 9. PMID: 24927539 [2] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	V12G01_RS25440
Coordinate (Strand)	108650..112039 (+)
NCBI ID	491515732
RefSeq	NZ_CH902589
Uniprot ID	UPI0000D545DA
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	3.4e-49	154..408
IcmF-related	PF06761.14	1.8e-42	485..755
IcmF_C	PF06744.14	8.8e-19	1004..1107

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	MFKQKL
Transmembrane helix	7-29	FRNPVVISTILMFLVAAIITTIY
Outside	30-38	LLFLDEIDQ
Transmembrane helix	39-61	LYLWLTLGAIAIVYVIFQFTLWL
Inside	62-401	KKKNNAKAQHLETEEEALSMVIQPLLANSGKKPIYLMLGNKGSGRRQFLFNSSAIKPMDRTRTAKNDFFEWYESDSAVYIKPDQRLVFQEVSSSDASLWNTFIEEVIHHRPRKPFSGCLFFIDFEFMIVSEPEQKEYTISSLLARLSTINSKTSSALPIYLIMSKLDKLDGFKEYMHFSSLKTRVEFLSIPLKEAKGVFAEYYRDSYRNLVRVLESNALDASANSTDIDEKQAILAFPKQFELCQSEVSYVLERLCDVNNGVYSLDIREIFFSSSLQGGRKYNLLAKSCSNYFNLPIIASEHTQLTETPYFSRFLIDSQILPESDFAGENKTYLRLIQRQ
Transmembrane helix	402-424	SRLAILGSIILLTGGSYFFFTTL
Outside	425-1129	DSNLHVMNQLLSIDDKSQVEQDARFNELLAKATKAIEPSYGAWLKGSKALDEEILPLNISRLDQSTRIAYEALLKDVAQQLIPLIEKGYRLQLTQNQNTFTRALPLLKGYLMLNDPTKRDIRFLRHQTLQMMNDLSNQNDVVNQAMRYLDAYFRTQFDPIDINMDLVRATRRSLLANSNVDLVYAGILNEANSIDLGTLDLQRAVGFEFSNVFNTPIDSERLIVDKIYTSTGFSTFYRPRVDLMSQQVISDNWVLGLSQHVIPTSEEQEAFKEEVRKKYTDDYISNWRNALSELKVQNYDNVGDLTNAIDLISGPSSPMTTVLKQVYANTQFSPVGEKNALISKLDPKLVAAADSASDAVEEVVKPDYLLMKRVEQAFHLLNQLQVSETPNSPTPWDETVTALSRVRTYMKDIADAPDPQMAALAAAQHRMNSSEADPLIKLKQIAQKSPEPVRSWLLDVVQQSWSVMIKESAKGIQTQWYSEIYTKFKEIGLGRYPFDLTATEEISIEDFELLFASGGLLDTFIQKNFAPFYDTNLWTPKQVDGETMPISPALLVQLRNYNVIRDTLINKSTNRVHIPFSARVLDLDSSAIRASLKIADTNISYYHGPSRIREMAWPPQNGDFNISITLQDVTDEGRQHVLNKSGQWAIYRLLGDSTLTNTHNGSFVSDIKVSGRDLSLRITPLTQKNPFTLAELYNFTLPESI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9959	-	-

Protein sequence: 1130 a.a.

						>T6CP015491 NZ_CH902589:108650-112039 [Vibrio alginolyticus 12G01] [TssM]
MFKQKLFRNPVVISTILMFLVAAIITTIYLLFLDEIDQLYLWLTLGAIAIVYVIFQFTLWLKKKNNAKAQHLETEEEALS
MVIQPLLANSGKKPIYLMLGNKGSGRRQFLFNSSAIKPMDRTRTAKNDFFEWYESDSAVYIKPDQRLVFQEVSSSDASLW
NTFIEEVIHHRPRKPFSGCLFFIDFEFMIVSEPEQKEYTISSLLARLSTINSKTSSALPIYLIMSKLDKLDGFKEYMHFS
SLKTRVEFLSIPLKEAKGVFAEYYRDSYRNLVRVLESNALDASANSTDIDEKQAILAFPKQFELCQSEVSYVLERLCDVN
NGVYSLDIREIFFSSSLQGGRKYNLLAKSCSNYFNLPIIASEHTQLTETPYFSRFLIDSQILPESDFAGENKTYLRLIQR
QSRLAILGSIILLTGGSYFFFTTLDSNLHVMNQLLSIDDKSQVEQDARFNELLAKATKAIEPSYGAWLKGSKALDEEILP
LNISRLDQSTRIAYEALLKDVAQQLIPLIEKGYRLQLTQNQNTFTRALPLLKGYLMLNDPTKRDIRFLRHQTLQMMNDLS
NQNDVVNQAMRYLDAYFRTQFDPIDINMDLVRATRRSLLANSNVDLVYAGILNEANSIDLGTLDLQRAVGFEFSNVFNTP
IDSERLIVDKIYTSTGFSTFYRPRVDLMSQQVISDNWVLGLSQHVIPTSEEQEAFKEEVRKKYTDDYISNWRNALSELKV
QNYDNVGDLTNAIDLISGPSSPMTTVLKQVYANTQFSPVGEKNALISKLDPKLVAAADSASDAVEEVVKPDYLLMKRVEQ
AFHLLNQLQVSETPNSPTPWDETVTALSRVRTYMKDIADAPDPQMAALAAAQHRMNSSEADPLIKLKQIAQKSPEPVRSW
LLDVVQQSWSVMIKESAKGIQTQWYSEIYTKFKEIGLGRYPFDLTATEEISIEDFELLFASGGLLDTFIQKNFAPFYDTN
LWTPKQVDGETMPISPALLVQLRNYNVIRDTLINKSTNRVHIPFSARVLDLDSSAIRASLKIADTNISYYHGPSRIREMA
WPPQNGDFNISITLQDVTDEGRQHVLNKSGQWAIYRLLGDSTLTNTHNGSFVSDIKVSGRDLSLRITPLTQKNPFTLAEL
YNFTLPESI*					

Nucleotide sequence: 3390 bp

						>T6CP015491 NZ_CH902589:108650-112039 [Vibrio alginolyticus 12G01] [TssM]
ATGTTCAAACAAAAACTGTTTAGAAATCCAGTGGTGATTTCTACCATATTAATGTTTTTAGTGGCTGCAATTATCACCAC
TATTTATCTGCTTTTTCTTGACGAAATCGATCAACTTTATCTTTGGCTGACCCTTGGTGCTATCGCCATTGTTTACGTCA
TTTTTCAATTCACATTATGGTTGAAGAAAAAGAACAACGCTAAAGCTCAACATTTGGAAACCGAGGAAGAAGCACTTTCT
ATGGTTATCCAGCCTCTTCTTGCTAACTCAGGCAAGAAACCAATTTACCTGATGCTTGGTAATAAAGGGTCAGGGCGACG
TCAATTTTTATTTAATTCTAGTGCAATCAAACCGATGGATCGCACTAGAACCGCTAAGAATGACTTTTTTGAATGGTACG
AATCTGACAGCGCTGTATACATCAAACCAGACCAACGCTTAGTGTTCCAGGAAGTATCAAGCAGTGATGCTTCTTTATGG
AATACGTTCATTGAAGAAGTTATTCATCATCGACCTAGAAAGCCATTTTCTGGGTGTCTATTCTTCATCGATTTCGAGTT
CATGATCGTCTCTGAACCAGAACAAAAAGAGTACACCATTTCGTCGTTATTGGCGCGCTTGAGTACCATCAACTCTAAAA
CGTCTTCAGCATTGCCAATCTATTTGATTATGTCAAAGCTAGACAAGTTAGATGGCTTTAAAGAATACATGCACTTTAGC
TCTCTCAAGACACGTGTTGAGTTCCTCTCTATCCCACTAAAAGAAGCCAAGGGTGTATTTGCAGAGTACTACCGCGATAG
CTATCGAAATCTAGTGAGAGTGCTTGAAAGTAATGCGCTAGATGCATCCGCTAATTCAACGGACATTGATGAGAAACAAG
CAATACTCGCTTTCCCTAAGCAGTTTGAGCTATGTCAGTCTGAAGTCAGCTATGTGCTTGAGCGTCTATGTGATGTTAAC
AACGGTGTCTATTCACTAGACATCCGTGAGATTTTCTTTAGTTCGAGTCTGCAAGGCGGTCGCAAGTACAATTTACTTGC
AAAAAGTTGTAGTAATTACTTCAACTTACCTATTATCGCGTCAGAGCATACACAGTTAACAGAAACGCCTTATTTCTCTC
GCTTTCTGATTGATTCACAAATATTGCCGGAAAGCGACTTTGCGGGCGAAAACAAGACGTATCTTCGTCTAATTCAGAGA
CAAAGCCGTTTGGCAATACTCGGCTCTATTATCCTGCTTACTGGGGGCAGTTACTTCTTTTTCACGACATTAGACAGTAA
CTTACATGTGATGAATCAACTCTTGAGTATCGATGACAAATCGCAAGTGGAGCAAGATGCAAGATTCAATGAGCTTTTAG
CGAAGGCGACGAAAGCAATAGAGCCTTCTTATGGGGCGTGGCTTAAAGGTTCTAAGGCTTTGGATGAAGAGATATTACCA
CTCAACATTAGCCGTTTGGATCAAAGCACTCGCATCGCATACGAAGCATTACTTAAGGACGTTGCTCAACAGTTGATACC
TCTCATTGAGAAAGGTTACCGTCTACAACTGACACAAAATCAAAACACTTTTACACGCGCTTTACCTCTATTGAAGGGGT
ATCTAATGCTAAACGATCCAACAAAGCGTGATATCCGTTTTTTGCGTCATCAAACTCTCCAAATGATGAACGACTTGAGC
AACCAAAATGACGTCGTCAATCAGGCGATGCGCTATTTGGACGCTTACTTTCGCACACAGTTTGATCCAATTGATATCAA
TATGGATCTTGTTCGCGCAACACGTCGCTCATTGCTAGCAAATTCGAATGTTGATCTCGTTTATGCAGGCATTTTAAACG
AAGCAAATTCGATTGATTTAGGAACCCTAGATCTGCAACGAGCTGTGGGTTTTGAGTTTAGCAATGTGTTCAACACACCA
ATCGATAGCGAACGCTTGATCGTAGATAAGATCTATACCTCCACTGGTTTTAGCACTTTTTATCGACCACGCGTGGACCT
AATGTCACAACAAGTCATCAGTGACAACTGGGTTTTAGGTCTGTCACAACACGTTATTCCTACTAGTGAAGAACAAGAAG
CCTTCAAAGAGGAAGTACGTAAAAAATATACTGACGACTACATCAGTAATTGGCGCAATGCGCTGAGTGAACTAAAAGTC
CAGAACTATGACAATGTCGGCGATCTAACGAACGCGATAGACTTAATCTCGGGCCCTTCTTCACCAATGACAACCGTACT
GAAGCAGGTCTATGCCAACACACAATTTTCACCCGTTGGTGAAAAAAATGCTCTGATTTCAAAGCTAGACCCGAAACTCG
TGGCAGCGGCAGACTCCGCATCAGACGCAGTAGAGGAAGTAGTTAAGCCTGATTACTTGTTGATGAAACGTGTTGAACAA
GCTTTTCACTTATTAAACCAACTTCAGGTCAGTGAAACACCAAACTCCCCTACTCCTTGGGATGAGACCGTAACAGCGCT
AAGCCGTGTTCGCACGTATATGAAAGACATTGCTGATGCACCCGACCCGCAAATGGCGGCGCTAGCGGCAGCTCAGCATC
GAATGAACAGCAGTGAAGCTGACCCATTAATCAAACTTAAACAGATTGCCCAAAAATCACCGGAACCCGTTCGTAGCTGG
TTGTTAGATGTGGTTCAACAAAGCTGGTCAGTCATGATTAAAGAGTCCGCTAAAGGCATTCAAACCCAATGGTACAGCGA
GATTTACACCAAGTTTAAAGAGATAGGGCTAGGACGATACCCATTTGATCTAACAGCGACCGAGGAAATCTCAATTGAAG
ATTTTGAATTGCTGTTTGCTTCCGGTGGTTTGTTAGACACATTTATTCAGAAAAATTTTGCACCGTTCTACGATACCAAT
TTGTGGACGCCAAAACAGGTGGATGGCGAGACGATGCCAATCTCTCCGGCTCTGTTAGTGCAACTGCGTAACTATAACGT
TATTCGAGACACATTAATTAATAAAAGCACTAACCGCGTTCATATTCCGTTTAGTGCAAGAGTACTCGATCTTGATTCTA
GTGCGATTAGAGCGAGCTTAAAAATTGCAGATACCAATATAAGTTACTACCACGGTCCAAGCCGCATTCGTGAAATGGCA
TGGCCACCGCAAAATGGTGACTTCAATATTAGTATCACACTCCAGGATGTAACCGATGAAGGCAGACAGCACGTTTTAAA
TAAGAGTGGTCAATGGGCGATATACAGATTGCTAGGTGACTCGACCTTGACAAACACTCATAACGGCAGCTTTGTCAGTG
ACATAAAAGTGTCAGGGAGGGACTTGAGCTTACGCATCACTCCGTTGACACAGAAAAACCCATTCACTCTTGCTGAGCTG
TATAACTTTACGCTCCCAGAGTCGATTTAA					