SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015486
Component protein type	TssL
T6SS ID (Type)	T6SS01224 (Type i1)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (264 a.a.); Nucleotide sequence (792 bp)
Reference	[1] Salomon D, Kinch LN, Trudgian DC, et al. Marker for type VI secretion system effectors. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6. doi: 10.1073/pnas.1406110111. Epub 2014 Jun 9. PMID: 24927539 [2] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	V12G01_RS25420
Coordinate (Strand)	102086..102877 (-)
NCBI ID	491515721
RefSeq	NZ_CH902589
Uniprot ID	A0A2I3CPN9_VIBAX, C0LZM0_VIBAL
KEGG ID	vag:N646_3998
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	1.4e-50	47..246

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-230	MSYAETDVTVVLFQPEPGKPMEVMPAPDLSRNIAVQDLSIDNMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAREIRKGERKLNEMELDRASVLVIRYCLCAAIDESVCRQEWGANSHWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRHVIEFLYLLLQLGFQGKYGREERGNEKLAEIGSTIYRLVRDDRLAEQEKVSLINLKAKYLKKPLKRVISPK
Transmembrane helix	231-253	LILSLSAMTFALMYAATYIVIDL
Outside	254-263	KFQQLLEMYR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9950	-	-

Protein sequence: 264 a.a.

						>T6CP015486 NZ_CH902589:c102877-102086 [Vibrio alginolyticus 12G01] [TssL]
MSYAETDVTVVLFQPEPGKPMEVMPAPDLSRNIAVQDLSIDNMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAR
EIRKGERKLNEMELDRASVLVIRYCLCAAIDESVCRQEWGANSHWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRH
VIEFLYLLLQLGFQGKYGREERGNEKLAEIGSTIYRLVRDDRLAEQEKVSLINLKAKYLKKPLKRVISPKLILSLSAMTF
ALMYAATYIVIDLKFQQLLEMYR*					

Nucleotide sequence: 792 bp

						>T6CP015486 NZ_CH902589:c102877-102086 [Vibrio alginolyticus 12G01] [TssL]
ATGAGTTACGCAGAAACCGACGTCACCGTAGTCTTATTCCAACCAGAGCCTGGCAAACCAATGGAGGTAATGCCAGCGCC
TGACTTGTCGAGAAACATCGCAGTACAAGATCTATCTATCGATAACATGGGTATTAACCCGCTAGTGGACCAGTTCTCAT
GGTTGATCGCTAGTCTGTCTTGTATGTCTTCGATCCCTTGGCTTGATGACCCGATGCCATTTCGTGAACAAGTGGCACGT
GAGATTCGAAAAGGTGAACGAAAACTTAATGAAATGGAGCTCGATCGTGCTTCGGTTCTTGTTATTCGTTACTGCTTGTG
TGCGGCGATTGATGAATCTGTTTGTCGGCAAGAGTGGGGGGCAAATAGTCACTGGAGCCAGAACAGCTTACTGTCTGAAT
TCCATAACGAAACATCTGGTGGTGACAAGTTTTTCGTTATTCTCGACCGCTTAAAAGCCGACCCTCGCAAATACCGTCAC
GTTATAGAGTTTCTCTATTTATTGCTTCAACTTGGTTTCCAAGGCAAGTACGGCCGTGAAGAGCGTGGTAACGAAAAGTT
AGCGGAAATTGGCAGCACCATTTATCGATTAGTGCGAGATGACCGCCTAGCAGAACAAGAAAAAGTTTCTTTAATCAACC
TTAAAGCTAAATACCTAAAGAAGCCGTTAAAAAGGGTAATTTCACCCAAGCTGATCTTGAGTCTTAGTGCGATGACGTTT
GCGCTTATGTATGCGGCAACCTACATCGTCATAGACTTAAAATTTCAGCAACTGCTTGAAATGTATAGATAA					