SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015458
Component protein type	TssJ
T6SS ID (Type)	T6SS01222 (Type i3)
Strain	Pantoea ananatis LMG 2665
Replicon	chromosome
Sequence	Protein sequence (168 a.a.); Nucleotide sequence (504 bp)
Reference	[1] Shyntum DY, Theron J, Venter SN, et al. Pantoea ananatis Utilizes a Type VI Secretion System for Pathogenesis and Bacterial Competition. Mol Plant Microbe Interact. 2015 Apr;28(4):420-31. doi: 10.1094/MPMI-07-14-0219-R. PMID: 25411959

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	N454_00637
Coordinate (Strand)	42792..43295 (-)
NCBI ID	574093492
GenBank	KF590027
Uniprot ID	W8DUB9_PANAN
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.3e-21	34..137

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-167	MAQTYFRVGLAKWIWPFLLLLLTACSSSKPDIARYNLHFQAHPSINSGAPLKVRVMLLTSDAEFMSADFFTLQNQPATALGNTLLNNQQFFLMSGQLSKTLSAKSLPDARFIGIMAEYQKLDGKVWRLALPFPDSDSSSFWQFWKSNDGELNANIIVDDAGMRPVKQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9992	pos: 24-25. LTA-CS.	MAQTYFRVGLAKWIWPFLLLLLTA

Protein sequence: 168 a.a.

						>T6CP015458 KF590027:c43295-42792 [Pantoea ananatis LMG 2665] [TssJ]
MAQTYFRVGLAKWIWPFLLLLLTACSSSKPDIARYNLHFQAHPSINSGAPLKVRVMLLTSDAEFMSADFFTLQNQPATAL
GNTLLNNQQFFLMSGQLSKTLSAKSLPDARFIGIMAEYQKLDGKVWRLALPFPDSDSSSFWQFWKSNDGELNANIIVDDA
GMRPVKQ*					

Nucleotide sequence: 504 bp

						>T6CP015458 KF590027:c43295-42792 [Pantoea ananatis LMG 2665] [TssJ]
ATGGCACAAACTTACTTTCGCGTTGGCCTGGCAAAATGGATCTGGCCCTTCCTGCTGCTTCTGCTAACGGCATGCAGTAG
CAGTAAGCCTGATATTGCGCGATACAACCTGCACTTCCAGGCACATCCTTCCATCAATTCGGGCGCACCTCTCAAAGTCC
GGGTGATGTTGCTGACGTCTGATGCAGAATTTATGTCCGCCGACTTCTTCACTCTGCAGAATCAACCCGCTACCGCACTG
GGAAATACGCTGCTCAACAATCAGCAGTTTTTCCTGATGTCAGGTCAGCTCAGTAAAACGCTGAGTGCCAAAAGCCTCCC
CGACGCGCGCTTTATCGGCATCATGGCGGAATATCAAAAGCTGGATGGCAAGGTGTGGCGACTTGCGCTGCCTTTCCCAG
ACAGTGACAGCAGCTCGTTCTGGCAGTTCTGGAAAAGCAATGATGGTGAGTTAAACGCCAACATTATCGTTGACGATGCC
GGCATGCGTCCCGTTAAGCAATAA					