Detailed information of component protein


Summary

Component ID T6CP015456
Component protein type TssL
T6SS ID (Type) T6SS01222 experimental (Type i3)
Strain Pantoea ananatis LMG 2665
Replicon chromosome
Sequence Protein sequence (414 a.a.); Nucleotide sequence (1242 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) N454_00635
Coordinate (Strand) 40172..41413 (-)
NCBI ID 574093490
GenBank KF590027
Uniprot ID W8DUL8_PANAN
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1.5e-69 23..224
OmpA PF00691.22 5e-15 291..389
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-201 MMQEQNKPNSDAFQQDLSHQNSLVAAANPLINAIPQIRHSVSHDDPARLRQQLIDQIRRFELACQQSGLSYEVIVGARYCLCTALDEAAALTPWGSRGVWTSNGLLVTFHNETWGGEKFFQLLAKLSQNPRQHILLLELIYFCLLLGFEGRYRVLDNGRSQLDTIKQRLLQMIRSVRGSFAAALSPHPTDQPVLRKLWRPM
Transmembrane helix 202-224 IPLWACAALAGLAACLFYIVLNW
Outside 225-413 RLGDYTSPVLAKIYQTTLPEVQIRNPAPPPPPSLNLKSFLKPEIDEGLVAVRDEADQSVVTLKGDGLFASGSTEVRGRYDAVIQRVAEAMNNVSGKILVVGYSDNVPIRSARFASNYELSLARAQSVQTLLQKQLAKPSRVKAEGRGETHPLVPNTSAENRARNRRVEITLQVAPDTTQAEVNGLARGN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9694 - -


  Protein sequence: 414 a.a.    

>T6CP015456 KF590027:c41413-40172 [Pantoea ananatis LMG 2665] [TssL]
MMQEQNKPNSDAFQQDLSHQNSLVAAANPLINAIPQIRHSVSHDDPARLRQQLIDQIRRFELACQQSGLSYEVIVGARYC
LCTALDEAAALTPWGSRGVWTSNGLLVTFHNETWGGEKFFQLLAKLSQNPRQHILLLELIYFCLLLGFEGRYRVLDNGRS
QLDTIKQRLLQMIRSVRGSFAAALSPHPTDQPVLRKLWRPMIPLWACAALAGLAACLFYIVLNWRLGDYTSPVLAKIYQT
TLPEVQIRNPAPPPPPSLNLKSFLKPEIDEGLVAVRDEADQSVVTLKGDGLFASGSTEVRGRYDAVIQRVAEAMNNVSGK
ILVVGYSDNVPIRSARFASNYELSLARAQSVQTLLQKQLAKPSRVKAEGRGETHPLVPNTSAENRARNRRVEITLQVAPD
TTQAEVNGLARGN*

  Nucleotide sequence: 1242 bp    

>T6CP015456 KF590027:c41413-40172 [Pantoea ananatis LMG 2665] [TssL]
ATGATGCAGGAACAAAATAAACCGAACAGTGATGCTTTTCAGCAGGATCTTAGCCACCAGAATAGTTTGGTGGCTGCAGC
GAATCCGCTGATTAACGCGATTCCACAGATTCGTCACTCCGTCTCCCATGACGATCCCGCACGTTTACGTCAACAGCTGA
TTGACCAGATCCGCCGTTTCGAGCTGGCCTGCCAGCAGTCCGGACTGAGCTATGAGGTGATCGTTGGCGCGCGCTATTGT
CTGTGTACGGCGCTTGATGAAGCCGCAGCACTGACGCCCTGGGGAAGCCGTGGCGTGTGGACCAGTAATGGCTTACTGGT
AACCTTCCACAACGAAACCTGGGGCGGGGAGAAATTCTTCCAACTGCTGGCGAAACTGTCGCAAAACCCGCGTCAGCACA
TCCTGTTACTGGAGCTGATCTATTTCTGCTTACTGCTTGGTTTTGAAGGGCGCTATCGGGTTCTGGATAATGGACGCTCG
CAACTGGATACGATTAAACAGCGTCTGTTGCAGATGATCAGGAGCGTCAGGGGCAGCTTTGCCGCCGCCTTGTCGCCGCA
TCCGACCGATCAGCCGGTTCTGCGTAAACTCTGGCGCCCCATGATTCCGCTGTGGGCCTGTGCGGCTCTGGCGGGCCTGG
CTGCCTGCCTGTTCTATATCGTGCTGAACTGGCGTCTGGGGGATTACACCTCGCCCGTGCTGGCCAAAATCTACCAAACG
ACGCTGCCAGAAGTGCAGATTCGTAATCCGGCACCGCCGCCGCCGCCGTCACTGAACCTGAAGTCATTCCTGAAACCTGA
AATCGACGAAGGCCTGGTGGCCGTGCGCGATGAGGCAGATCAAAGTGTGGTGACGCTGAAAGGCGATGGCTTGTTCGCAT
CCGGTTCGACAGAGGTTCGCGGACGTTATGATGCCGTTATCCAGCGTGTAGCTGAAGCCATGAATAACGTCAGCGGGAAA
ATCCTGGTGGTGGGCTACAGCGATAACGTACCGATTCGCAGTGCCCGATTTGCCTCCAACTATGAGCTGTCTCTGGCCCG
TGCCCAGTCTGTTCAGACGCTGTTACAAAAACAGCTGGCAAAACCATCACGCGTTAAAGCGGAAGGGCGGGGTGAAACGC
ATCCGTTGGTGCCGAATACCAGTGCAGAAAATCGCGCGCGTAATCGTCGTGTTGAAATTACGCTGCAGGTTGCACCTGAT
ACGACGCAAGCTGAAGTTAACGGCCTGGCGAGAGGGAACTAA