SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015444
Component protein type	TssG
T6SS ID (Type)	T6SS01222 (Type i3)
Strain	Pantoea ananatis LMG 2665
Replicon	chromosome
Sequence	Protein sequence (350 a.a.); Nucleotide sequence (1050 bp)
Reference	[1] Shyntum DY, Theron J, Venter SN, et al. Pantoea ananatis Utilizes a Type VI Secretion System for Pathogenesis and Bacterial Competition. Mol Plant Microbe Interact. 2015 Apr;28(4):420-31. doi: 10.1094/MPMI-07-14-0219-R. PMID: 25411959

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	N454_00615
Coordinate (Strand)	18352..19401 (-)
NCBI ID	574093470
GenBank	KF590027
Uniprot ID	W8DUL0_PANAN
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.9e-101	27..329

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-349	MSETAHVIPLQRAVRLPDGFWQAVMAAPWRYDLFQLLRKLDAQSGQRYALGKAPLPKFEAVRIGQKPSLAFAPSTVSQVKAREHDERYDVSIYSFGLFGPNGPLPTHLTEYVRGRIDHHQDHSLSAFADLFHHRATLLFYRAWANSQPTSSLDRRDDRRFQDYLACVAGIGLPAQQKASSLSLHARLMLSGHLSRHGHDAEGLVRILRHYFAVPVKLQQNVPQWLSLDSRDQARLSAGRHMPRLGESAFLGVAVRDVQHRFRLRIGPLSAEQYERFLPNAQGACEVRDWVRHYLGIEMQWDLSLILAADAVKGVTLGGQARLGYTSWLGQQPQPVDREDFAFEVEATPS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9931	-	-

Protein sequence: 350 a.a.

						>T6CP015444 KF590027:c19401-18352 [Pantoea ananatis LMG 2665] [TssG]
MSETAHVIPLQRAVRLPDGFWQAVMAAPWRYDLFQLLRKLDAQSGQRYALGKAPLPKFEAVRIGQKPSLAFAPSTVSQVK
AREHDERYDVSIYSFGLFGPNGPLPTHLTEYVRGRIDHHQDHSLSAFADLFHHRATLLFYRAWANSQPTSSLDRRDDRRF
QDYLACVAGIGLPAQQKASSLSLHARLMLSGHLSRHGHDAEGLVRILRHYFAVPVKLQQNVPQWLSLDSRDQARLSAGRH
MPRLGESAFLGVAVRDVQHRFRLRIGPLSAEQYERFLPNAQGACEVRDWVRHYLGIEMQWDLSLILAADAVKGVTLGGQA
RLGYTSWLGQQPQPVDREDFAFEVEATPS*					

Nucleotide sequence: 1050 bp

						>T6CP015444 KF590027:c19401-18352 [Pantoea ananatis LMG 2665] [TssG]
ATGAGTGAGACGGCGCACGTTATTCCCCTACAGCGGGCGGTGCGTCTGCCTGACGGTTTCTGGCAGGCCGTGATGGCCGC
ACCCTGGCGCTATGATCTGTTTCAGCTGTTACGCAAGCTCGACGCCCAGAGTGGGCAGCGTTATGCCTTAGGTAAAGCGC
CACTGCCCAAATTTGAGGCCGTGCGTATCGGGCAAAAGCCATCTCTGGCCTTTGCACCATCGACCGTGTCACAGGTTAAA
GCGCGTGAGCATGACGAACGCTACGACGTCTCCATTTACAGCTTTGGGCTGTTTGGTCCTAACGGTCCTCTCCCTACGCA
CCTGACGGAATATGTGCGCGGACGTATCGATCACCATCAGGATCACAGCCTGAGCGCTTTCGCCGATCTGTTTCATCATC
GTGCCACGCTGTTGTTCTATCGTGCCTGGGCTAATTCACAGCCAACCAGCTCTCTGGATCGCCGCGACGATCGGCGCTTC
CAGGATTACCTGGCCTGCGTGGCCGGTATTGGGTTACCGGCCCAGCAGAAAGCCAGTTCGCTGAGCCTGCATGCGCGCCT
CATGCTGTCAGGGCATCTCAGCCGCCACGGTCACGATGCCGAAGGGCTGGTACGCATTTTGCGTCACTACTTTGCGGTTC
CGGTTAAGCTGCAACAGAACGTGCCGCAGTGGTTATCCCTGGACAGCCGCGATCAGGCCCGTCTGTCGGCCGGTCGCCAT
ATGCCGCGCCTGGGTGAATCGGCCTTTCTCGGCGTAGCCGTTCGTGATGTACAGCATCGTTTTCGTCTGCGCATCGGTCC
ACTGAGCGCCGAGCAGTACGAACGTTTTTTACCCAACGCGCAAGGCGCTTGCGAAGTGCGTGACTGGGTGCGCCACTATC
TGGGTATTGAGATGCAGTGGGATCTCAGCCTGATTCTGGCAGCCGATGCCGTTAAGGGCGTTACCCTTGGCGGTCAGGCG
CGGCTGGGCTACACCAGTTGGCTTGGACAGCAACCGCAGCCTGTCGATCGTGAAGATTTTGCCTTTGAGGTTGAAGCTAC
GCCGTCATAG					