Detailed information of component protein
Summary
| Component ID | T6CP015435 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS01220  (Type i1) |
| Strain | Campylobacter jejuni strain WP2202 |
| Replicon | plasmid pCJDM202 |
| Sequence | Protein sequence (258 a.a.); Nucleotide sequence (774 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | A0W69_09025 |
| Coordinate (Strand) | 24287..25060 (+) |
| NCBI ID | 1004801962 |
| GenBank | CP014743 |
| Uniprot ID | R9THY1_CAMJU |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 2.1e-36 | 27..234 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-214 | MKEINEEKKALSLLLDSDFDGLKNNKIIDYSLELLLLSYRLSKISSMDTSSINQLRETLINKILDITAKLSICKEYDEKEIIKFKYCLCVFIDESLMKNELFINFWAHNTLTVRLFDETLGGNNFYDIASSWINNPFKFKDFLEFIYACLILGYKGKYNETKDRDEKIIHFCNNIATSLKPVYKIEEELAFNKAYKTGLKENIWQKFIRLYFKK |
| Transmembrane helix | 215-233 | LIIVVPVLIILGVLSYSIF |
| Outside | 234-257 | NLETNNLKVDNNISVLIKNLTHIE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9972 | - | - |
Protein sequence: 258 a.a.
>T6CP015435 CP014743:24287-25060 [Campylobacter jejuni] [TssL]
MKEINEEKKALSLLLDSDFDGLKNNKIIDYSLELLLLSYRLSKISSMDTSSINQLRETLINKILDITAKLSICKEYDEKE
IIKFKYCLCVFIDESLMKNELFINFWAHNTLTVRLFDETLGGNNFYDIASSWINNPFKFKDFLEFIYACLILGYKGKYNE
TKDRDEKIIHFCNNIATSLKPVYKIEEELAFNKAYKTGLKENIWQKFIRLYFKKLIIVVPVLIILGVLSYSIFNLETNNL
KVDNNISVLIKNLTHIE*
MKEINEEKKALSLLLDSDFDGLKNNKIIDYSLELLLLSYRLSKISSMDTSSINQLRETLINKILDITAKLSICKEYDEKE
IIKFKYCLCVFIDESLMKNELFINFWAHNTLTVRLFDETLGGNNFYDIASSWINNPFKFKDFLEFIYACLILGYKGKYNE
TKDRDEKIIHFCNNIATSLKPVYKIEEELAFNKAYKTGLKENIWQKFIRLYFKKLIIVVPVLIILGVLSYSIFNLETNNL
KVDNNISVLIKNLTHIE*
Nucleotide sequence: 774 bp
>T6CP015435 CP014743:24287-25060 [Campylobacter jejuni] [TssL]
ATGAAAGAAATAAATGAAGAAAAAAAGGCATTAAGCTTACTTCTTGATTCAGATTTTGATGGTTTAAAAAACAATAAAAT
TATTGATTATTCTTTGGAGTTGCTTTTGCTTTCTTATAGACTTTCAAAAATAAGCTCTATGGATACAAGTAGTATTAATC
AACTTAGAGAAACATTGATTAATAAAATTTTAGATATAACCGCTAAATTAAGTATATGTAAAGAATATGATGAAAAAGAA
ATTATTAAATTCAAATATTGTCTTTGTGTCTTTATAGATGAAAGCTTAATGAAAAATGAACTTTTTATTAATTTTTGGGC
TCACAATACTCTAACTGTAAGGTTATTTGATGAAACTTTAGGCGGAAATAATTTTTATGATATTGCATCATCTTGGATTA
ATAACCCTTTTAAGTTTAAAGATTTTTTGGAATTTATCTATGCTTGTTTAATTCTTGGTTATAAAGGAAAATATAATGAG
ACTAAGGATAGAGATGAAAAAATAATTCATTTTTGCAATAATATTGCCACATCTTTAAAACCGGTTTATAAGATAGAAGA
AGAGTTAGCTTTTAATAAAGCATATAAAACTGGTTTGAAAGAAAATATTTGGCAAAAATTTATAAGATTGTATTTTAAAA
AACTTATTATCGTTGTGCCAGTATTGATTATATTAGGAGTATTAAGCTATTCAATTTTCAATTTAGAAACTAATAATCTT
AAAGTGGATAATAATATATCCGTATTGATAAAAAATTTAACACATATAGAATAA
ATGAAAGAAATAAATGAAGAAAAAAAGGCATTAAGCTTACTTCTTGATTCAGATTTTGATGGTTTAAAAAACAATAAAAT
TATTGATTATTCTTTGGAGTTGCTTTTGCTTTCTTATAGACTTTCAAAAATAAGCTCTATGGATACAAGTAGTATTAATC
AACTTAGAGAAACATTGATTAATAAAATTTTAGATATAACCGCTAAATTAAGTATATGTAAAGAATATGATGAAAAAGAA
ATTATTAAATTCAAATATTGTCTTTGTGTCTTTATAGATGAAAGCTTAATGAAAAATGAACTTTTTATTAATTTTTGGGC
TCACAATACTCTAACTGTAAGGTTATTTGATGAAACTTTAGGCGGAAATAATTTTTATGATATTGCATCATCTTGGATTA
ATAACCCTTTTAAGTTTAAAGATTTTTTGGAATTTATCTATGCTTGTTTAATTCTTGGTTATAAAGGAAAATATAATGAG
ACTAAGGATAGAGATGAAAAAATAATTCATTTTTGCAATAATATTGCCACATCTTTAAAACCGGTTTATAAGATAGAAGA
AGAGTTAGCTTTTAATAAAGCATATAAAACTGGTTTGAAAGAAAATATTTGGCAAAAATTTATAAGATTGTATTTTAAAA
AACTTATTATCGTTGTGCCAGTATTGATTATATTAGGAGTATTAAGCTATTCAATTTTCAATTTAGAAACTAATAATCTT
AAAGTGGATAATAATATATCCGTATTGATAAAAAATTTAACACATATAGAATAA