SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015433
Component protein type	TssJ
T6SS ID (Type)	T6SS01220 (Type i1)
Strain	Campylobacter jejuni strain WP2202
Replicon	plasmid pCJDM202
Sequence	Protein sequence (149 a.a.); Nucleotide sequence (447 bp)
Reference	[1] Marasini D, Karki AB, Bryant JM, et al. Molecular characterization of megaplasmids encoding the type VI secretion system in Campylobacter jejuni isolated from chicken livers and gizzards. Sci Rep. 2020 Jul 27;10(1):12514. doi: 10.1038/s41598-020-69155-z. PMID: 32719325

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	A0W69_09015
Coordinate (Strand)	22437..22883 (+)
NCBI ID	1004801960
GenBank	CP014743
Uniprot ID	R9TJM7_CAMJU
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	9.6e-14	23..126

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-148	MFCKKSLFFLLPLFFCACSSVVSVKINNVENSNLNNRHDDVPVTAIVYQLKDIKKFEEASDIDLATREEGVLGKDKLDSIKTQIAPKDNIIAVKVDEEEVPYVGILVLFANNTKKTTKIWAKTEDANGFGKNKYLKFEISKEGIKRIK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9948	pos: 17-18. FCA-CS.	MFCKKSLFFLLPLFFCA

Protein sequence: 149 a.a.

						>T6CP015433 CP014743:22437-22883 [Campylobacter jejuni] [TssJ]
MFCKKSLFFLLPLFFCACSSVVSVKINNVENSNLNNRHDDVPVTAIVYQLKDIKKFEEASDIDLATREEGVLGKDKLDSI
KTQIAPKDNIIAVKVDEEEVPYVGILVLFANNTKKTTKIWAKTEDANGFGKNKYLKFEISKEGIKRIK*					

Nucleotide sequence: 447 bp

						>T6CP015433 CP014743:22437-22883 [Campylobacter jejuni] [TssJ]
ATGTTTTGTAAAAAAAGCTTATTTTTTTTATTGCCACTATTCTTTTGCGCTTGCTCAAGTGTTGTTAGTGTTAAGATAAA
TAATGTAGAGAATTCCAATCTTAATAATAGACATGATGATGTTCCTGTTACGGCTATTGTATATCAACTTAAGGATATTA
AAAAATTTGAAGAAGCAAGTGATATAGATTTGGCTACTAGAGAAGAGGGGGTACTAGGCAAGGATAAACTTGATTCTATT
AAAACGCAAATTGCACCTAAAGACAATATTATCGCGGTTAAAGTAGATGAAGAAGAGGTTCCTTATGTAGGCATTCTTGT
ATTATTTGCAAATAATACAAAAAAAACAACAAAAATATGGGCAAAGACCGAAGATGCAAATGGGTTTGGGAAGAATAAGT
ATTTAAAATTTGAAATATCCAAAGAAGGTATCAAAAGAATTAAATAG					