SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015431
Component protein type	TssB
T6SS ID (Type)	T6SS01220 (Type i1)
Strain	Campylobacter jejuni strain WP2202
Replicon	plasmid pCJDM202
Sequence	Protein sequence (162 a.a.); Nucleotide sequence (486 bp)
Reference	[1] Marasini D, Karki AB, Bryant JM, et al. Molecular characterization of megaplasmids encoding the type VI secretion system in Campylobacter jejuni isolated from chicken livers and gizzards. Sci Rep. 2020 Jul 27;10(1):12514. doi: 10.1038/s41598-020-69155-z. PMID: 32719325

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	A0W69_09005
Coordinate (Strand)	20522..21007 (-)
NCBI ID	AMP64282.1
GenBank	CP014743
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	3.2e-52	6..154

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-161	MSDGSYAPKERINITYKAKTNGQNEDVELPLKLMVMANLKGKNETPLEEREILQINKINFDQVMRKLNITTSFSVKNTLGTGAEELDVKLNIASMKDFSPDSLAKQIPELNKLLQLREALMALKGPMGNIPDFRKAVLEALKNEKTKEKLLLEIKQEEQGN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9928	-	-

Protein sequence: 162 a.a.

						>T6CP015431 CP014743:c21007-20522 [Campylobacter jejuni] [TssB]
MSDGSYAPKERINITYKAKTNGQNEDVELPLKLMVMANLKGKNETPLEEREILQINKINFDQVMRKLNITTSFSVKNTLG
TGAEELDVKLNIASMKDFSPDSLAKQIPELNKLLQLREALMALKGPMGNIPDFRKAVLEALKNEKTKEKLLLEIKQEEQG
N*					

Nucleotide sequence: 486 bp

						>T6CP015431 CP014743:c21007-20522 [Campylobacter jejuni] [TssB]
ATGTCAGATGGATCTTATGCTCCAAAAGAGCGGATTAATATTACATATAAAGCTAAAACTAATGGACAAAATGAGGATGT
GGAGTTGCCGTTAAAATTAATGGTAATGGCAAATTTAAAAGGAAAAAACGAAACTCCTTTAGAGGAAAGAGAAATTTTGC
AAATTAATAAGATTAATTTTGATCAAGTCATGAGAAAACTCAATATAACAACAAGTTTTAGCGTAAAAAACACTCTTGGA
ACAGGAGCTGAAGAGCTTGATGTTAAGCTCAATATTGCTAGTATGAAGGATTTTTCTCCAGATAGCTTAGCTAAACAAAT
ACCCGAGCTAAACAAATTATTGCAGCTAAGAGAAGCATTAATGGCTTTAAAAGGTCCTATGGGTAATATTCCTGATTTTA
GAAAAGCTGTTTTAGAAGCGTTAAAAAATGAAAAAACTAAAGAAAAATTACTTTTAGAAATTAAACAAGAAGAGCAAGGA
AATTAA					