SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015428
Component protein type	TssF
T6SS ID (Type)	T6SS01220 (Type i1)
Strain	Campylobacter jejuni strain WP2202
Replicon	plasmid pCJDM202
Sequence	Protein sequence (574 a.a.); Nucleotide sequence (1722 bp)
Reference	[1] Marasini D, Karki AB, Bryant JM, et al. Molecular characterization of megaplasmids encoding the type VI secretion system in Campylobacter jejuni isolated from chicken livers and gizzards. Sci Rep. 2020 Jul 27;10(1):12514. doi: 10.1038/s41598-020-69155-z. PMID: 32719325

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	A0W69_08990
Coordinate (Strand)	16953..18674 (-)
NCBI ID	1004801955
GenBank	CP014743
Uniprot ID	UPI000779A20D
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	9.5e-116	6..569

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-573	MKDNIFYYQKELEYLYEKREYFIKNYPKLTPFLAYDSKDPDIERIIENLAILSSKIHQELDENIPHIAESLINIVSPNYTNPLPSLCMQEFKFEQNSKENNLIIPKGTLIKSKPIDKCVCEFKTVYDVYLYSISISEVFISSKNQDYTFNLTLQVNKAETKICDLGLEKINLYLGNDPYMSSTLLLYMHSYLKELKIQSLDTDEEFFLNTYNIEKIGLNPDESSLSYNDLGFEAFSLLREYFFMPHKFNFLRINGLDILNNCQGKTINIEFKFSKPFPANCIFRKELLSLSMTPIINIFTKSAEPLINNHKKDSYRIFVDRSQPKAYEIIQTLQVKAHNSEGGKRLLKNYKSFERFEFLKDNQKDFYSVNTKKNSKGEVFSEISFFSSYIMDETISIDLLCSNGDLPSKLKIGDINTCDLKGVDTKNVEIPSETRRCSVDGNLLWKLVSVLSFSYQTILSKKAFFGVLESYSFLDNQSNWKIYKLLQESIIDIQSKSTYLIDENITKKGTLAIFSIKDSKFYTLGEVYLLGLIISKFLASFASINSFCELKIRCLDSKEILHYPASFGKKALI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9936	-	-

Protein sequence: 574 a.a.

						>T6CP015428 CP014743:c18674-16953 [Campylobacter jejuni] [TssF]
MKDNIFYYQKELEYLYEKREYFIKNYPKLTPFLAYDSKDPDIERIIENLAILSSKIHQELDENIPHIAESLINIVSPNYT
NPLPSLCMQEFKFEQNSKENNLIIPKGTLIKSKPIDKCVCEFKTVYDVYLYSISISEVFISSKNQDYTFNLTLQVNKAET
KICDLGLEKINLYLGNDPYMSSTLLLYMHSYLKELKIQSLDTDEEFFLNTYNIEKIGLNPDESSLSYNDLGFEAFSLLRE
YFFMPHKFNFLRINGLDILNNCQGKTINIEFKFSKPFPANCIFRKELLSLSMTPIINIFTKSAEPLINNHKKDSYRIFVD
RSQPKAYEIIQTLQVKAHNSEGGKRLLKNYKSFERFEFLKDNQKDFYSVNTKKNSKGEVFSEISFFSSYIMDETISIDLL
CSNGDLPSKLKIGDINTCDLKGVDTKNVEIPSETRRCSVDGNLLWKLVSVLSFSYQTILSKKAFFGVLESYSFLDNQSNW
KIYKLLQESIIDIQSKSTYLIDENITKKGTLAIFSIKDSKFYTLGEVYLLGLIISKFLASFASINSFCELKIRCLDSKEI
LHYPASFGKKALI*					

Nucleotide sequence: 1722 bp

						>T6CP015428 CP014743:c18674-16953 [Campylobacter jejuni] [TssF]
ATGAAGGATAATATTTTTTATTATCAAAAAGAGCTTGAATATCTATATGAAAAAAGAGAGTATTTTATAAAAAATTATCC
AAAGTTAACACCATTTTTGGCATATGATAGCAAAGATCCTGATATAGAAAGAATTATTGAAAATTTAGCTATTTTAAGCT
CTAAAATCCATCAAGAGCTTGATGAAAATATACCACACATTGCAGAATCTTTGATTAATATTGTTTCTCCAAATTATACA
AATCCTTTACCTTCTTTATGCATGCAAGAATTTAAATTTGAACAAAATAGTAAAGAAAATAATTTAATTATTCCCAAAGG
AACTCTTATTAAATCAAAACCAATAGACAAATGTGTTTGTGAATTTAAAACAGTATATGATGTTTATCTTTATTCTATCT
CCATCAGCGAAGTTTTTATATCTAGTAAAAATCAAGATTATACTTTCAATTTAACTTTGCAAGTCAATAAGGCAGAAACA
AAAATATGTGATTTGGGTTTGGAGAAAATTAATTTATATCTAGGCAATGATCCCTATATGTCTAGTACTTTATTATTATA
CATGCATTCTTATTTAAAAGAATTAAAAATTCAAAGTTTAGATACTGATGAAGAATTTTTTCTTAATACCTATAATATTG
AAAAAATAGGATTGAATCCAGATGAAAGTAGTTTATCTTATAATGATTTAGGTTTTGAAGCATTTTCCTTACTAAGAGAA
TATTTTTTCATGCCACATAAATTTAATTTTTTAAGAATCAATGGTTTGGATATTTTAAATAATTGTCAAGGAAAGACAAT
TAATATTGAATTTAAATTCTCTAAACCATTTCCTGCAAATTGTATTTTTAGAAAAGAATTACTTTCTTTAAGCATGACAC
CTATTATCAATATCTTTACAAAAAGTGCAGAACCTCTTATAAATAATCACAAAAAAGATAGTTATAGAATTTTTGTGGAT
AGAAGCCAACCTAAAGCTTACGAAATCATACAGACACTACAGGTTAAAGCACATAATAGTGAAGGCGGCAAAAGGTTATT
AAAAAACTATAAAAGTTTTGAACGCTTTGAATTTTTAAAAGATAATCAAAAGGATTTTTATAGCGTAAATACTAAAAAGA
ATTCCAAAGGAGAGGTATTTAGCGAAATTTCTTTCTTTTCCTCTTATATAATGGATGAAACTATAAGTATTGATCTTTTG
TGCTCCAATGGAGATTTACCTTCAAAATTAAAAATAGGTGATATTAATACCTGCGATCTAAAAGGTGTCGATACCAAAAA
TGTGGAAATTCCAAGCGAAACAAGAAGATGTAGTGTTGATGGTAATTTATTATGGAAGCTTGTTTCTGTGTTATCTTTTT
CATATCAAACAATATTGAGCAAAAAAGCTTTTTTTGGTGTGTTGGAAAGCTATAGCTTTTTAGATAATCAATCAAATTGG
AAGATTTATAAGCTTTTGCAAGAATCTATAATCGATATTCAAAGTAAAAGCACTTATTTAATAGATGAAAATATCACAAA
AAAAGGGACCTTAGCTATTTTTAGTATTAAAGATTCGAAGTTTTACACTTTAGGCGAGGTATATTTATTGGGATTGATTA
TATCTAAATTTTTAGCTTCTTTTGCTAGTATTAACTCCTTTTGTGAGTTAAAAATAAGATGCCTTGACTCAAAAGAAATT
TTACATTACCCTGCTTCTTTTGGAAAAAAAGCTTTAATATGA					