SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015427
Component protein type	TssG
T6SS ID (Type)	T6SS01220 (Type i1)
Strain	Campylobacter jejuni strain WP2202
Replicon	plasmid pCJDM202
Sequence	Protein sequence (303 a.a.); Nucleotide sequence (909 bp)
Reference	[1] Marasini D, Karki AB, Bryant JM, et al. Molecular characterization of megaplasmids encoding the type VI secretion system in Campylobacter jejuni isolated from chicken livers and gizzards. Sci Rep. 2020 Jul 27;10(1):12514. doi: 10.1038/s41598-020-69155-z. PMID: 32719325

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	A0W69_08985
Coordinate (Strand)	16048..16956 (-)
NCBI ID	1004801954
GenBank	CP014743
Uniprot ID	A0A1E7P7Y1_CAMJU
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	2.1e-46	6..287

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-302	MNNLTSYSFFKLIKKLEKDYGRKNIFLRTNKSLKHPNKDIEKIIFSEHEQSIIELFINFMGLHGVSSQLPSFMLDKLSRNEDGDQGWTLFFDFFNHYLLWIFFDVISLKNYPRSFNENFKDSISKILFSMLGIKEYDIAKKYLPFAPLLLSLRRPKTHIERVLQVNFKLKDKLSIIENLPHQILISNSQKNNLGIKNNILGNNFILGDKFISYQNKIAIYIKDISYQEAVKFMPNGTKHDDLKNSIMFLTNNEFCVDLYLKINYSSEMKFVLGEENTAKLGWAKILGNTQKKYTIVYMKLCE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9960	-	-

Protein sequence: 303 a.a.

						>T6CP015427 CP014743:c16956-16048 [Campylobacter jejuni] [TssG]
MNNLTSYSFFKLIKKLEKDYGRKNIFLRTNKSLKHPNKDIEKIIFSEHEQSIIELFINFMGLHGVSSQLPSFMLDKLSRN
EDGDQGWTLFFDFFNHYLLWIFFDVISLKNYPRSFNENFKDSISKILFSMLGIKEYDIAKKYLPFAPLLLSLRRPKTHIE
RVLQVNFKLKDKLSIIENLPHQILISNSQKNNLGIKNNILGNNFILGDKFISYQNKIAIYIKDISYQEAVKFMPNGTKHD
DLKNSIMFLTNNEFCVDLYLKINYSSEMKFVLGEENTAKLGWAKILGNTQKKYTIVYMKLCE*					

Nucleotide sequence: 909 bp

						>T6CP015427 CP014743:c16956-16048 [Campylobacter jejuni] [TssG]
ATGAATAATCTTACTTCATATAGTTTTTTTAAATTAATAAAAAAATTAGAAAAAGACTATGGAAGAAAGAATATATTTTT
AAGAACAAATAAAAGTTTAAAACATCCAAATAAAGATATAGAAAAAATCATTTTTTCAGAACACGAACAATCAATTATAG
AATTGTTTATAAATTTCATGGGGCTACATGGAGTCTCTTCACAACTTCCTTCATTTATGCTAGATAAACTGTCAAGAAAT
GAAGATGGCGATCAAGGTTGGACTCTATTCTTTGATTTCTTTAATCATTATTTGCTTTGGATATTTTTTGATGTAATAAG
CTTAAAAAATTATCCACGATCTTTTAATGAAAATTTTAAAGATTCTATTTCAAAAATTTTATTTAGCATGTTGGGAATTA
AAGAATACGATATTGCAAAAAAATATTTACCATTTGCACCTTTACTACTAAGTTTAAGACGACCTAAAACACACATTGAA
AGAGTTTTACAAGTCAATTTTAAACTTAAGGATAAATTATCAATTATTGAAAATTTACCCCATCAAATTTTAATTTCAAA
TTCGCAAAAAAATAACTTAGGAATAAAGAACAATATTTTAGGAAATAATTTCATATTAGGCGATAAATTTATCTCTTATC
AAAACAAAATAGCGATCTATATAAAGGATATTTCATATCAAGAAGCGGTTAAATTTATGCCCAATGGAACCAAACACGAT
GATTTAAAAAATAGTATAATGTTTTTAACAAATAATGAATTTTGTGTTGATTTATATCTTAAAATCAACTATTCTTCAGA
AATGAAGTTTGTGTTAGGAGAAGAAAATACTGCCAAACTTGGTTGGGCAAAAATTTTAGGTAATACGCAAAAAAAATATA
CAATAGTATATATGAAACTATGTGAATAA					