Detailed information of component protein
Summary
| Component ID | T6CP015377 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01215  (Type i2) |
| Strain | Klebsiella pneumoniae strain Kp52.145 |
| Replicon | chromosome |
| Sequence | Protein sequence (181 a.a.); Nucleotide sequence (543 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BN49_RS14095 |
| Coordinate (Strand) | 2588864..2589406 (+) |
| NCBI ID | WP_004176417.1 |
| RefSeq | NZ_FO834906 |
| Uniprot ID | A0A0H3GS07_KLEPH, A0A7U9CZW7_KLEPN, W1HXN0_KLEPN, A0A652Q1B5_9ENTR, A0A0E9Z527_KLEPN, A0A377ZH20_KLEPO, A0A1Y0Q0Z0_KLEPN |
| KEGG ID | kpnu:LI86_14515, kpv:KPNIH29_11490, kpy:KPNIH31_11120, kpg:KPNIH32_11920, kpb:FH42_03005, kpne:KU54_014580, kpw:KPNIH30_12080, kpm:KPHS_23190 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 1.9e-27 | 47..163 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-180 | VLRISLTKTACLLPLLALCLSGCGLTQRVSEGTKSAFNAVFYKKINTLHLDFTAREALNTDARENHSLSEPVVVRIYQLKDRQTFDRLVYQQLLEEGDILLAADLLASRDVVIGPGGDASLNMPLEAEATFVAVVGLFRHPDTQRNTWKQVLAREELDPDKPRIFTAEHNQLRLRPEAAK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9981 | pos: 22-23. LSG-CG. | VLRISLTKTACLLPLLALCLSG |
Protein sequence: 181 a.a.
>T6CP015377 NZ_FO834906:2588864-2589406 [Klebsiella pneumoniae] [TssJ]
VLRISLTKTACLLPLLALCLSGCGLTQRVSEGTKSAFNAVFYKKINTLHLDFTAREALNTDARENHSLSEPVVVRIYQLK
DRQTFDRLVYQQLLEEGDILLAADLLASRDVVIGPGGDASLNMPLEAEATFVAVVGLFRHPDTQRNTWKQVLAREELDPD
KPRIFTAEHNQLRLRPEAAK*
VLRISLTKTACLLPLLALCLSGCGLTQRVSEGTKSAFNAVFYKKINTLHLDFTAREALNTDARENHSLSEPVVVRIYQLK
DRQTFDRLVYQQLLEEGDILLAADLLASRDVVIGPGGDASLNMPLEAEATFVAVVGLFRHPDTQRNTWKQVLAREELDPD
KPRIFTAEHNQLRLRPEAAK*
Nucleotide sequence: 543 bp
>T6CP015377 NZ_FO834906:2588864-2589406 [Klebsiella pneumoniae] [TssJ]
GTGTTACGTATAAGTTTGACTAAAACCGCCTGTCTGCTGCCCCTGCTTGCGCTTTGCCTGAGCGGATGCGGACTCACCCA
GCGCGTCTCGGAGGGAACAAAGTCTGCCTTCAACGCCGTTTTTTATAAGAAAATTAATACGCTGCATCTGGACTTTACCG
CGCGCGAAGCGCTCAACACCGATGCCAGAGAGAATCACTCGCTCTCTGAACCGGTGGTGGTGCGCATCTATCAGCTTAAA
GATCGCCAGACCTTTGACCGGCTGGTTTACCAGCAGCTGCTGGAGGAGGGGGATATTCTGCTCGCCGCCGATCTCCTCGC
CAGTCGCGATGTGGTGATCGGTCCCGGAGGGGACGCCAGTCTGAATATGCCGCTGGAAGCGGAGGCGACGTTCGTGGCGG
TTGTCGGCCTGTTCCGCCATCCGGACACACAAAGAAATACCTGGAAGCAGGTGCTGGCGCGTGAGGAATTGGATCCGGAC
AAGCCGCGCATTTTCACCGCCGAACACAACCAGCTACGGCTCAGGCCTGAAGCGGCGAAGTAA
GTGTTACGTATAAGTTTGACTAAAACCGCCTGTCTGCTGCCCCTGCTTGCGCTTTGCCTGAGCGGATGCGGACTCACCCA
GCGCGTCTCGGAGGGAACAAAGTCTGCCTTCAACGCCGTTTTTTATAAGAAAATTAATACGCTGCATCTGGACTTTACCG
CGCGCGAAGCGCTCAACACCGATGCCAGAGAGAATCACTCGCTCTCTGAACCGGTGGTGGTGCGCATCTATCAGCTTAAA
GATCGCCAGACCTTTGACCGGCTGGTTTACCAGCAGCTGCTGGAGGAGGGGGATATTCTGCTCGCCGCCGATCTCCTCGC
CAGTCGCGATGTGGTGATCGGTCCCGGAGGGGACGCCAGTCTGAATATGCCGCTGGAAGCGGAGGCGACGTTCGTGGCGG
TTGTCGGCCTGTTCCGCCATCCGGACACACAAAGAAATACCTGGAAGCAGGTGCTGGCGCGTGAGGAATTGGATCCGGAC
AAGCCGCGCATTTTCACCGCCGAACACAACCAGCTACGGCTCAGGCCTGAAGCGGCGAAGTAA