Detailed information of component protein


Summary

Component ID T6CP015373
Component protein type TssM
T6SS ID (Type) T6SS01215 experimental (Type i2)
Strain Klebsiella pneumoniae strain Kp52.145
Replicon chromosome
Sequence Protein sequence (1132 a.a.); Nucleotide sequence (3396 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BN49_RS14075
Coordinate (Strand) 2581020..2584415 (+)
NCBI ID WP_032102817.1
RefSeq NZ_FO834906
Uniprot ID W9BP39_KLEPN
KEGG ID kpnk:BN49_2475
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 1.3e-60 405..722
IcmF_C PF06744.14 1.8e-21 981..1085
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MRKNIR
Transmembrane helix 7-29 LVGFGLLILVILFLIVLLISQIN
Outside 30-43 KIPAVIEGYSTWLK
Transmembrane helix 44-63 IGAIIGISCISCITAQFVLS
Inside 64-356 ERHIETPFTPDDENYVNEMYYATAENNNELKKYLQHRYSFFWHRKVRLLLVTGDEAAIEQLVPGLRQQRWLEGQRTVLIYGGSLLSEPDSEQYAALRKLRRGRPLDGIVRVMPSSLTLTPQISESDLHGLEKISELLGYAAPVWLWKLCDSEWPQADRAVQAVGVSFPLRATEEDVARQLAQMLPTLREQGMHQIAADTRHDFLLRLGQQLIDGGIAQWRWQLAPWLTASRQRHALRGLMFSLPEPRTVDPYQEADTSPAGQPHLLTLPATWLGIVDDCRRLRGHHVGMAWER
Transmembrane helix 357-379 GLACGLLAILGLWAAGLLLSFAL
Outside 380-1131 NYSQIASVAGKARDLVAHPSVSDYQLTALHALRNEAGRLVHDGQKGAPWYRRFGLDHHQQLLNAVLPWYGVANHRLIRDPANAALQQALSALVNSAPNSDQRARLAKPGYDQLKAWLMMARPDKADGAFFAQTMKTVQPTRMGISTGLWQSLAPDLWAFYLSLLPERPEWKIIPDAQRVSQSRQVLLQQLGRRNAESTLYENMLKSVRRNFADVSLEDMTSGTDARRLFTTDEVVPGMFTRQAWEGGIQQAIDKAASSRREEIDWVLSDSRKTMSTDLSPEALKARLTRRYFTDFAGSWLNFLNSLRLNPATNIADVTDQLTLISDVRQSPLIALMNTLAWQGQAGQQRKGLSDSLIKSAKDLVGGKDKPVIDQSAAGPQGPLDDTFGPLLQLMGKNTGSNVMSADSSLSLQTYLTRITRVRLRLQQVANASDPLEMMQTLAQTVFQGKSVDLTDTQQYGSLISASLGEEWSGFGNTLFVQPLTQAWETVLLPSATSLNDKWRRSVVANWHTAFDGRFPFAASKSDASLPMLAEFVRKDSGRIERFLTTELNGVLHKEGSHWVPDKVNSHGLVFNPAFLRAINQLSQLSDILFTDGSQGISFELQARPAPEIVETQLTIDGQKLRYFNQMADWQTFRWPGETYKPGTLLTWTTVNAGTRLFGDYSGTWGFIRWLEQGKRHPLDRSQWMMSFSAPDGRTLQWVLRSQLGSGPLALLALRGLTLPDQIFTVDAAESAQTLTTGLGNSDMDEMEL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9848 - -


  Protein sequence: 1132 a.a.    

>T6CP015373 NZ_FO834906:2581020-2584415 [Klebsiella pneumoniae] [TssM]
MRKNIRLVGFGLLILVILFLIVLLISQINKIPAVIEGYSTWLKIGAIIGISCISCITAQFVLSERHIETPFTPDDENYVN
EMYYATAENNNELKKYLQHRYSFFWHRKVRLLLVTGDEAAIEQLVPGLRQQRWLEGQRTVLIYGGSLLSEPDSEQYAALR
KLRRGRPLDGIVRVMPSSLTLTPQISESDLHGLEKISELLGYAAPVWLWKLCDSEWPQADRAVQAVGVSFPLRATEEDVA
RQLAQMLPTLREQGMHQIAADTRHDFLLRLGQQLIDGGIAQWRWQLAPWLTASRQRHALRGLMFSLPEPRTVDPYQEADT
SPAGQPHLLTLPATWLGIVDDCRRLRGHHVGMAWERGLACGLLAILGLWAAGLLLSFALNYSQIASVAGKARDLVAHPSV
SDYQLTALHALRNEAGRLVHDGQKGAPWYRRFGLDHHQQLLNAVLPWYGVANHRLIRDPANAALQQALSALVNSAPNSDQ
RARLAKPGYDQLKAWLMMARPDKADGAFFAQTMKTVQPTRMGISTGLWQSLAPDLWAFYLSLLPERPEWKIIPDAQRVSQ
SRQVLLQQLGRRNAESTLYENMLKSVRRNFADVSLEDMTSGTDARRLFTTDEVVPGMFTRQAWEGGIQQAIDKAASSRRE
EIDWVLSDSRKTMSTDLSPEALKARLTRRYFTDFAGSWLNFLNSLRLNPATNIADVTDQLTLISDVRQSPLIALMNTLAW
QGQAGQQRKGLSDSLIKSAKDLVGGKDKPVIDQSAAGPQGPLDDTFGPLLQLMGKNTGSNVMSADSSLSLQTYLTRITRV
RLRLQQVANASDPLEMMQTLAQTVFQGKSVDLTDTQQYGSLISASLGEEWSGFGNTLFVQPLTQAWETVLLPSATSLNDK
WRRSVVANWHTAFDGRFPFAASKSDASLPMLAEFVRKDSGRIERFLTTELNGVLHKEGSHWVPDKVNSHGLVFNPAFLRA
INQLSQLSDILFTDGSQGISFELQARPAPEIVETQLTIDGQKLRYFNQMADWQTFRWPGETYKPGTLLTWTTVNAGTRLF
GDYSGTWGFIRWLEQGKRHPLDRSQWMMSFSAPDGRTLQWVLRSQLGSGPLALLALRGLTLPDQIFTVDAAESAQTLTTG
LGNSDMDEMEL*

  Nucleotide sequence: 3396 bp    

>T6CP015373 NZ_FO834906:2581020-2584415 [Klebsiella pneumoniae] [TssM]
ATGAGAAAAAACATACGATTGGTAGGTTTTGGTCTCCTGATTTTGGTTATCTTGTTCCTGATTGTTTTATTAATTTCTCA
GATAAATAAAATCCCTGCTGTTATTGAGGGATATTCCACTTGGTTAAAGATAGGCGCGATTATAGGAATATCTTGCATTT
CCTGCATAACAGCTCAGTTCGTCTTATCTGAGAGGCATATTGAAACACCATTTACCCCTGATGATGAGAACTATGTCAAC
GAGATGTATTATGCGACGGCAGAGAACAACAATGAATTAAAAAAATATCTTCAGCACCGCTATAGTTTCTTTTGGCACCG
CAAAGTCCGCCTGCTGCTGGTTACCGGCGACGAGGCGGCAATCGAACAACTGGTGCCAGGCCTGCGACAGCAGCGCTGGC
TGGAGGGCCAGCGTACCGTTCTGATTTACGGCGGCAGTCTGCTCTCCGAGCCCGACAGTGAGCAATATGCCGCGCTGCGC
AAGCTGCGCCGTGGCCGTCCTCTGGACGGGATTGTGCGGGTGATGCCGTCGTCTCTCACCCTGACGCCGCAGATAAGCGA
GAGCGACCTGCACGGGCTGGAAAAAATCAGCGAACTGCTGGGCTATGCCGCCCCGGTCTGGTTATGGAAACTGTGCGACA
GTGAGTGGCCGCAGGCTGACCGCGCGGTGCAGGCGGTAGGGGTTAGCTTCCCGTTGCGCGCCACTGAGGAAGATGTCGCC
CGTCAGCTTGCGCAGATGTTGCCCACGCTACGGGAGCAGGGGATGCACCAGATCGCAGCAGATACCCGCCACGACTTTCT
GCTGCGTCTGGGGCAGCAGCTTATCGACGGGGGGATCGCGCAATGGCGCTGGCAGCTGGCGCCGTGGCTGACCGCCTCGC
GGCAGCGGCATGCGCTGCGAGGCCTGATGTTTAGCCTGCCAGAGCCTCGTACCGTAGATCCGTATCAGGAGGCGGATACC
TCGCCTGCGGGCCAGCCTCATCTCCTGACGCTGCCCGCCACCTGGCTGGGGATCGTCGATGACTGCCGCCGTTTACGCGG
GCATCACGTGGGAATGGCGTGGGAGCGGGGCCTGGCGTGCGGTCTGCTGGCCATCCTCGGTCTGTGGGCGGCGGGACTGC
TGCTCTCCTTTGCCCTCAATTACAGTCAGATTGCTTCGGTAGCGGGAAAAGCCCGCGACCTGGTGGCCCACCCCTCCGTT
TCTGACTATCAACTGACGGCCCTCCATGCCCTGCGCAATGAGGCCGGCCGTCTCGTGCACGATGGGCAGAAGGGAGCGCC
CTGGTATCGGCGCTTTGGTCTGGACCATCATCAGCAACTATTAAACGCCGTCCTGCCCTGGTACGGTGTGGCAAATCATC
GCCTGATACGTGACCCGGCCAATGCCGCGTTGCAGCAGGCGCTCAGCGCGCTGGTCAACTCCGCCCCCAATAGCGACCAA
CGAGCCAGGCTGGCGAAACCGGGCTATGACCAGCTGAAAGCCTGGCTGATGATGGCTCGTCCGGATAAAGCCGATGGCGC
ATTTTTTGCGCAGACCATGAAAACGGTCCAGCCGACGCGAATGGGGATCTCGACCGGTCTGTGGCAAAGTCTGGCGCCGG
ATTTGTGGGCCTTTTATCTCTCCCTGCTGCCCGAGCGGCCAGAGTGGAAGATCATCCCGGATGCGCAACGGGTGAGTCAG
AGCCGTCAGGTTCTGTTGCAGCAGCTAGGTCGCCGCAATGCCGAAAGCACCTTATATGAGAACATGCTCAAATCCGTGCG
TCGTAATTTTGCCGATGTGTCGCTGGAAGACATGACCAGTGGCACCGACGCGCGCCGCCTGTTTACCACCGATGAAGTGG
TCCCGGGGATGTTTACCCGCCAGGCATGGGAAGGTGGCATTCAGCAGGCCATTGATAAGGCAGCCAGCTCGCGCCGGGAG
GAAATCGACTGGGTGCTGAGCGATTCGCGCAAAACGATGTCGACAGACCTGTCGCCGGAGGCGCTGAAAGCGCGTCTGAC
CCGACGCTACTTCACCGATTTCGCCGGCAGCTGGCTGAATTTTCTCAACAGTCTGCGACTTAATCCGGCGACCAATATCG
CCGATGTTACCGACCAGCTCACGCTGATTAGCGATGTCCGCCAGTCGCCGCTGATTGCCCTGATGAATACCCTTGCCTGG
CAGGGGCAGGCGGGGCAACAAAGGAAGGGGCTGTCGGACAGCCTTATTAAATCGGCCAAAGATCTGGTGGGCGGGAAAGA
CAAGCCGGTCATCGACCAGTCGGCAGCCGGCCCGCAGGGGCCTCTGGATGACACGTTCGGCCCACTGCTCCAGCTGATGG
GGAAAAACACGGGCAGCAACGTCATGTCGGCCGATAGCTCCCTGAGCCTGCAAACCTATCTGACCCGTATCACCCGCGTG
CGCCTGCGCCTGCAGCAGGTTGCGAATGCCTCCGATCCGCTGGAGATGATGCAGACTCTGGCGCAGACCGTCTTCCAGGG
GAAAAGCGTCGACCTGACCGACACGCAACAGTACGGTAGCCTGATTTCGGCAAGCCTTGGCGAAGAGTGGAGTGGCTTCG
GCAACACTCTGTTTGTGCAGCCGCTGACTCAGGCCTGGGAAACCGTGCTTCTGCCGTCGGCGACCAGTCTGAATGATAAA
TGGCGCCGTTCGGTGGTCGCCAACTGGCACACCGCCTTTGACGGACGCTTTCCGTTTGCCGCCAGCAAAAGCGACGCCTC
CCTGCCGATGCTGGCTGAGTTTGTGCGCAAAGACAGCGGGCGAATCGAACGTTTTCTCACGACGGAGCTGAACGGTGTCC
TGCATAAAGAAGGTAGTCACTGGGTGCCGGATAAGGTCAACAGTCACGGCCTGGTCTTTAATCCGGCCTTTCTGCGCGCG
ATCAACCAGCTAAGCCAGCTGTCCGACATTCTTTTCACCGACGGCAGCCAGGGGATCAGCTTTGAGTTACAGGCCAGGCC
TGCCCCGGAGATTGTCGAGACACAGCTGACGATAGACGGCCAAAAGCTGCGCTATTTCAATCAGATGGCCGACTGGCAGA
CTTTCCGCTGGCCGGGGGAGACGTATAAACCCGGCACTCTGCTGACCTGGACCACCGTCAATGCGGGCACGCGTCTGTTC
GGTGACTACAGCGGTACCTGGGGTTTTATTCGCTGGCTGGAGCAGGGTAAACGCCATCCGCTGGATCGCAGCCAGTGGAT
GATGAGCTTCTCCGCTCCGGATGGCCGCACCCTGCAGTGGGTATTGCGCTCCCAGCTTGGGAGTGGCCCGCTGGCGCTTC
TGGCGCTGCGCGGATTGACCCTGCCGGATCAGATTTTCACTGTCGATGCGGCTGAGAGCGCACAGACCCTGACGACGGGG
TTAGGGAATAGTGACATGGATGAGATGGAATTATGA