SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015365
Component protein type	TssL
T6SS ID (Type)	T6SS01214 (Type Unknown)
Strain	Klebsiella pneumoniae strain Kp52.145
Replicon	chromosome
Sequence	Protein sequence (222 a.a.); Nucleotide sequence (666 bp)
Reference	[1] Storey D, McNally A, Åstrand M, et al. Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent. PLoS Pathog. 2020 Mar 19;16(3):e1007969. doi: 10.1371/journal.ppat.1007969. eCollection 2020 Mar. PMID: 32191774

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BN49_RS06035
Coordinate (Strand)	970193..970858 (-)
NCBI ID	WP_004181140.1
RefSeq	NZ_FO834906
Uniprot ID	W9BCQ1_KLEPN
KEGG ID	kpnk:BN49_0946, kpx:PMK1_00652
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	1.3e-43	12..207

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-185	MTQERKIDIDVLLRDTFLTVVELRQGTSARHGQELYRHCLQQVEGVRERLTAAGFSQQDIEHITYAQCALLDETVLSRDVMDDGQMVWLKNPLQSHFFNTLQAGELLYERMKRFLQEPAPTPAVLTCFHRVLLLGFRGRYQDPESDERQHLIAMFSKRVEPFRVQEDSAVLNVPLTRRQHYLWQS
Transmembrane helix	186-208	PFCWLLLLMLVLVGVWWGLHSWL
Outside	209-221	NVLVDELLPTGKS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9877	-	-

Protein sequence: 222 a.a.

						>T6CP015365 NZ_FO834906:c970858-970193 [Klebsiella pneumoniae] [TssL]
MTQERKIDIDVLLRDTFLTVVELRQGTSARHGQELYRHCLQQVEGVRERLTAAGFSQQDIEHITYAQCALLDETVLSRDV
MDDGQMVWLKNPLQSHFFNTLQAGELLYERMKRFLQEPAPTPAVLTCFHRVLLLGFRGRYQDPESDERQHLIAMFSKRVE
PFRVQEDSAVLNVPLTRRQHYLWQSPFCWLLLLMLVLVGVWWGLHSWLNVLVDELLPTGKS*					

Nucleotide sequence: 666 bp

						>T6CP015365 NZ_FO834906:c970858-970193 [Klebsiella pneumoniae] [TssL]
ATGACACAGGAACGGAAAATCGACATTGACGTACTACTTCGCGACACCTTTCTGACGGTGGTGGAACTGCGTCAGGGCAC
TTCTGCAAGACACGGACAGGAACTATATCGCCATTGTCTGCAGCAGGTGGAAGGGGTACGCGAGCGTCTGACAGCGGCCG
GATTCAGCCAGCAGGACATAGAACATATCACTTACGCTCAGTGCGCGCTGCTGGACGAAACCGTTCTCAGCCGGGACGTG
ATGGATGATGGCCAGATGGTCTGGCTAAAAAACCCTCTCCAGTCGCATTTTTTCAATACCCTTCAGGCGGGCGAACTACT
GTATGAGCGTATGAAGCGGTTTTTACAGGAGCCAGCCCCCACGCCGGCGGTCCTGACCTGTTTTCATCGCGTGCTGTTGC
TGGGGTTTCGCGGGCGCTATCAGGACCCCGAATCTGATGAACGGCAGCATCTCATTGCGATGTTCAGCAAGCGGGTTGAG
CCATTCAGGGTGCAAGAAGATTCGGCAGTGCTGAACGTGCCTTTGACCAGGCGTCAGCATTACCTGTGGCAGTCTCCTTT
TTGCTGGCTGCTACTGCTAATGCTTGTGCTGGTTGGCGTCTGGTGGGGGTTGCATAGCTGGCTGAATGTACTGGTGGATG
AACTGCTGCCAACAGGCAAGTCTTGA					