SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015360
Component protein type	TssG
T6SS ID (Type)	T6SS01214 (Type Unknown)
Strain	Klebsiella pneumoniae strain Kp52.145
Replicon	chromosome
Sequence	Protein sequence (361 a.a.); Nucleotide sequence (1083 bp)
Reference	[1] Storey D, McNally A, Åstrand M, et al. Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent. PLoS Pathog. 2020 Mar 19;16(3):e1007969. doi: 10.1371/journal.ppat.1007969. eCollection 2020 Mar. PMID: 32191774

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BN49_RS05965
Coordinate (Strand)	952292..953374 (-)
NCBI ID	WP_004181150.1
RefSeq	NZ_FO834906
Uniprot ID	W9B3E9_KLEPN
KEGG ID	kpnk:BN49_0933, kpx:PMK1_00660
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	4.3e-75	20..319

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-360	MDRTPQPARTGLTERLAPDINRINFYRFCQLLEQSQPQTPLLGSTTNPADDPVRFRPHPGMGFPVSELKSVEQDLDHPDAPPTVRTTFLGLYGVDSPLPTAYLDYIAQRRDGHEAMMAFLDIFNHRFITQYYRIWRKYNYLTSFEPGAVDNISRCLLGLIGLGIPGSENHIATPVSRFLALLSVMRLPTRTAEGITALVRLLAPQTQATVIPHDPQQIVLPEPAGLSKRSRICLKTRALLGNTGTDVNSQLLMKLYTEDDGEARGWLPGGQLHTDLLVLLRVYLGWRYQARLQLTLPVSLLPPAQLGNQRVQVSRTGVLRASFAAPVAGTVTVSLGRYQGLMPTSSTRDRESVTHVSYTL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9805	-	-

Protein sequence: 361 a.a.

						>T6CP015360 NZ_FO834906:c953374-952292 [Klebsiella pneumoniae] [TssG]
MDRTPQPARTGLTERLAPDINRINFYRFCQLLEQSQPQTPLLGSTTNPADDPVRFRPHPGMGFPVSELKSVEQDLDHPDA
PPTVRTTFLGLYGVDSPLPTAYLDYIAQRRDGHEAMMAFLDIFNHRFITQYYRIWRKYNYLTSFEPGAVDNISRCLLGLI
GLGIPGSENHIATPVSRFLALLSVMRLPTRTAEGITALVRLLAPQTQATVIPHDPQQIVLPEPAGLSKRSRICLKTRALL
GNTGTDVNSQLLMKLYTEDDGEARGWLPGGQLHTDLLVLLRVYLGWRYQARLQLTLPVSLLPPAQLGNQRVQVSRTGVLR
ASFAAPVAGTVTVSLGRYQGLMPTSSTRDRESVTHVSYTL*					

Nucleotide sequence: 1083 bp

						>T6CP015360 NZ_FO834906:c953374-952292 [Klebsiella pneumoniae] [TssG]
ATGGACAGAACACCACAGCCAGCGCGTACCGGGCTGACGGAAAGGCTTGCTCCGGACATTAACCGAATTAACTTTTACCG
TTTCTGCCAGCTTCTTGAACAAAGCCAACCACAGACGCCACTGCTGGGGAGCACAACCAATCCGGCAGACGATCCTGTCC
GTTTTCGTCCACATCCGGGGATGGGGTTCCCGGTAAGCGAACTAAAAAGCGTCGAACAGGATTTGGATCATCCTGACGCG
CCACCCACGGTGCGCACCACGTTTCTGGGGCTGTACGGTGTGGATTCACCGCTGCCGACAGCCTATCTCGACTACATCGC
GCAGCGCCGGGACGGCCATGAAGCGATGATGGCGTTTCTGGACATTTTTAACCATCGCTTTATCACTCAGTACTATCGTA
TCTGGCGCAAATATAATTACCTTACGTCGTTTGAACCGGGAGCCGTGGATAATATTTCCCGCTGCCTGCTGGGGTTGATT
GGTCTGGGCATTCCCGGCAGCGAAAATCATATCGCCACGCCGGTCTCGCGTTTTCTGGCATTACTCAGCGTGATGCGTCT
GCCGACGCGTACAGCAGAAGGCATAACGGCGCTGGTCAGATTACTGGCCCCCCAGACGCAAGCGACAGTTATCCCGCACG
ATCCACAACAAATTGTCCTGCCTGAACCTGCGGGTTTATCAAAAAGATCACGTATTTGCCTGAAAACTCGGGCACTGCTG
GGCAACACCGGCACTGACGTTAACAGCCAGTTACTGATGAAGCTGTATACCGAAGACGACGGAGAGGCACGCGGCTGGCT
GCCGGGCGGGCAACTACATACCGACCTGCTGGTGCTGTTGCGTGTCTATCTGGGCTGGCGCTACCAGGCCCGCCTGCAAC
TGACCTTGCCCGTCAGTCTGCTGCCTCCGGCACAGTTAGGAAATCAGCGCGTTCAGGTAAGCCGAACGGGCGTGCTGCGG
GCGTCTTTTGCTGCCCCAGTCGCCGGTACGGTCACGGTCAGTCTCGGGCGTTATCAGGGACTCATGCCCACATCATCCAC
CCGCGACAGGGAGAGTGTTACTCATGTTTCTTACACCCTATAA					