Detailed information of component protein


Summary

Component ID T6CP015355
Component protein type TssH
T6SS ID (Type) T6SS01213 experimental (Type i3)
Strain Pseudomonas aeruginosa PAK
Replicon chromosome
Sequence Protein sequence (903 a.a.); Nucleotide sequence (2709 bp)
Reference
[1] Chen R, Weng Y, Zhu F, et al. Polynucleotide Phosphorylase Regulates Multiple Virulence Factors and the Stabilities of Small RNAs RsmY/Z in Pseudomonas aeruginosa. Front Microbiol. 2016 Mar 2;7:247. doi: 10.3389/fmicb.2016.00247. eCollection 2016. PMID: 26973625
[2] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572
[3] Brencic A, Lory S. Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Mol Microbiol. 2009 May;72(3):612-32. doi: 10.1111/j.1365-2958.2009.06670.x. PMID: 19426209
[4] Marden JN, Diaz MR, Walton WG, et al. An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15055-60. doi: 10.1073/pnas.1307217110. Epub 2013 Aug 26. PMID: 23980177
[5] Aragon IM, Pérez-Mendoza D, Moscoso JA, et al. Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections. Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28. PMID: 25809128
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) Y880_RS27060
Coordinate (Strand) 5687208..5689916 (+)
NCBI ID WP_003115056.1
RefSeq NZ_CP020659
Uniprot ID CLPV1_PSEAE
KEGG ID pae:PA0090
PDB ID 4UQW
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 2.2e-38 631..797
AAA_lid_9 PF17871.3 4.3e-28 373..468
ClpB_D2-small PF10431.11 2.1e-12 804..878
AAA PF00004.31 2.6e-09 234..367
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-902 MSEISRVALFGKLNSLAYKAIEAATVFCKLRGNPYVELVHWFHQILQLPDSDLHQIVRQSGIDPARLAKDLTEALDRLPRGSTSITDLSSHVEEAVERGWVYGSLMFGESQVRTGYLVIGILKTPSLRHALTGLSAEFAKLKVEALTERFDEYVGASPENGLSASDGFNAGAAPGEASGALAPSAMGKQEALKRFTVDLTEQARSGKLDPIVGRDEEIRQLVDILMRRRQNNPILTGEAGVGKTAVVEGFALRIVAGDVPPALKDVELRALDVGLLQAGASMKGEFEQRLRQVIEDVQSSEKPIILFIDEAHTLVGAGGAAGTGDAANLLKPALARGTLRTVAATTWAEYKKHIEKDPALTRRFQVVQVDEPSEHKAILMMRGVASTMEKHHQVQILDEALEAAVRLSHRYIPARQLPDKSVSLLDTACARTAISLHAVPAEVDDSRRRIEALETELAIIRRESAIGVATAERQRNAETLLAEERERLAALEQRWAEEKRLVDELLETRARLRAAAEAVDAGGVPLGEGEVRLDEEQRQALHARLAELQAQLSALQGEEPLILPTVDYQAVASVVADWTGIPVGRMARNEIETVLNLDRHLKKRIIGQDHALEMIAKRIQTSRAGLDNPSKPIGVFMLAGTSGVGKTETALALAEAMYGGEQNVITINMSEFQEAHTVSTLKGAPPGYIGYGEGGVLTEAVRRKPYSVVLLDEVEKAHPDVHEIFFQVFDKGVMEDGEGRVIDFKNTLILLTTNAGTEMIASLCADPELMPEPEAIAKSLREPLLKIFPPALLGRLVTIPYYPLSDDMLKAISRLQLGRIKKRVEATHKVPFEFDEGVVDLIVSRCTETESGGRMIDAILTNTLLPDMSREFLTRMLEGKPLAGVRISSRDNQFHYDFAEAE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9908 - -


  Protein sequence: 903 a.a.    

>T6CP015355 NZ_CP020659:5687208-5689916 [Pseudomonas aeruginosa PAK] [TssH]
MSEISRVALFGKLNSLAYKAIEAATVFCKLRGNPYVELVHWFHQILQLPDSDLHQIVRQSGIDPARLAKDLTEALDRLPR
GSTSITDLSSHVEEAVERGWVYGSLMFGESQVRTGYLVIGILKTPSLRHALTGLSAEFAKLKVEALTERFDEYVGASPEN
GLSASDGFNAGAAPGEASGALAPSAMGKQEALKRFTVDLTEQARSGKLDPIVGRDEEIRQLVDILMRRRQNNPILTGEAG
VGKTAVVEGFALRIVAGDVPPALKDVELRALDVGLLQAGASMKGEFEQRLRQVIEDVQSSEKPIILFIDEAHTLVGAGGA
AGTGDAANLLKPALARGTLRTVAATTWAEYKKHIEKDPALTRRFQVVQVDEPSEHKAILMMRGVASTMEKHHQVQILDEA
LEAAVRLSHRYIPARQLPDKSVSLLDTACARTAISLHAVPAEVDDSRRRIEALETELAIIRRESAIGVATAERQRNAETL
LAEERERLAALEQRWAEEKRLVDELLETRARLRAAAEAVDAGGVPLGEGEVRLDEEQRQALHARLAELQAQLSALQGEEP
LILPTVDYQAVASVVADWTGIPVGRMARNEIETVLNLDRHLKKRIIGQDHALEMIAKRIQTSRAGLDNPSKPIGVFMLAG
TSGVGKTETALALAEAMYGGEQNVITINMSEFQEAHTVSTLKGAPPGYIGYGEGGVLTEAVRRKPYSVVLLDEVEKAHPD
VHEIFFQVFDKGVMEDGEGRVIDFKNTLILLTTNAGTEMIASLCADPELMPEPEAIAKSLREPLLKIFPPALLGRLVTIP
YYPLSDDMLKAISRLQLGRIKKRVEATHKVPFEFDEGVVDLIVSRCTETESGGRMIDAILTNTLLPDMSREFLTRMLEGK
PLAGVRISSRDNQFHYDFAEAE*

  Nucleotide sequence: 2709 bp    

>T6CP015355 NZ_CP020659:5687208-5689916 [Pseudomonas aeruginosa PAK] [TssH]
ATGAGTGAGATCAGTCGCGTTGCGCTGTTCGGCAAGCTGAACAGCCTGGCCTACAAGGCCATCGAGGCCGCCACGGTATT
CTGCAAGCTGCGCGGCAATCCCTATGTCGAGCTGGTGCACTGGTTCCACCAGATCCTCCAGTTGCCGGACTCGGACCTGC
ACCAGATCGTCCGCCAGTCCGGGATCGACCCGGCGCGCCTGGCCAAGGACCTGACCGAGGCCCTCGACCGTCTGCCGCGC
GGCTCGACCTCGATCACCGACCTGTCCTCCCATGTCGAGGAAGCGGTGGAGCGCGGCTGGGTCTACGGCAGCCTGATGTT
CGGCGAGAGCCAGGTGCGCACCGGCTACCTGGTGATCGGCATCCTCAAGACGCCGAGCCTGCGCCATGCCCTCACCGGGC
TGTCGGCGGAATTCGCCAAGCTCAAGGTCGAGGCGCTCACCGAGCGCTTCGACGAGTACGTCGGCGCCTCGCCGGAGAAC
GGCCTGTCGGCCAGCGACGGCTTCAACGCCGGCGCCGCCCCGGGCGAGGCCAGCGGCGCCCTGGCGCCGAGCGCGATGGG
CAAGCAGGAGGCGCTCAAGCGCTTTACCGTGGACCTCACCGAGCAGGCCCGTAGCGGCAAGCTCGACCCCATCGTCGGGC
GTGACGAGGAGATCCGCCAGTTGGTGGACATCCTCATGCGCCGCCGGCAGAACAACCCGATCCTCACCGGCGAGGCCGGC
GTCGGCAAGACCGCGGTGGTGGAAGGCTTCGCCCTGCGCATCGTCGCCGGCGACGTCCCGCCGGCGCTGAAGGACGTCGA
ACTGCGCGCCCTCGACGTCGGCCTGCTGCAGGCCGGGGCGAGCATGAAGGGCGAGTTCGAGCAGCGCCTGCGCCAGGTGA
TCGAAGACGTGCAGAGTTCCGAGAAGCCGATCATCCTGTTCATCGACGAAGCCCACACCCTGGTCGGCGCCGGTGGCGCG
GCCGGCACCGGCGACGCCGCCAACCTGCTCAAGCCGGCCCTGGCGCGCGGCACCCTGCGCACCGTGGCAGCGACCACCTG
GGCCGAGTACAAGAAGCACATCGAGAAGGACCCGGCGCTGACCCGGCGTTTCCAGGTGGTGCAGGTGGACGAGCCGTCCG
AGCACAAGGCCATCCTGATGATGCGCGGCGTCGCGTCGACCATGGAGAAGCACCACCAGGTGCAGATCCTCGACGAGGCG
CTGGAGGCGGCGGTCCGGCTGTCGCACCGCTACATCCCGGCGCGCCAGCTGCCGGACAAGTCGGTGAGCCTGCTGGATAC
CGCCTGCGCGCGCACCGCCATCAGCCTGCACGCGGTGCCGGCCGAGGTCGACGATAGCCGCCGTCGCATCGAGGCGCTGG
AAACCGAGTTGGCGATCATCCGCCGCGAGAGCGCCATCGGCGTCGCCACCGCCGAACGCCAGCGCAACGCCGAGACCCTG
CTGGCCGAGGAGCGCGAGCGCCTGGCCGCCCTGGAGCAGCGCTGGGCCGAGGAAAAACGCCTGGTCGACGAACTGCTGGA
GACCCGCGCCCGTCTGCGTGCCGCCGCCGAAGCGGTGGACGCCGGCGGCGTGCCGCTGGGCGAAGGCGAGGTGCGTCTCG
ACGAGGAACAGCGCCAGGCGCTGCATGCGCGGCTGGCGGAGCTGCAGGCGCAACTGAGCGCGTTGCAGGGCGAAGAGCCG
CTGATCCTGCCGACCGTGGACTACCAGGCGGTGGCCTCGGTGGTCGCCGACTGGACCGGCATCCCGGTCGGCCGCATGGC
GCGCAACGAGATCGAGACCGTGCTAAACCTCGACCGGCACCTGAAGAAGCGCATCATCGGCCAGGACCACGCGCTGGAGA
TGATCGCCAAGCGCATCCAGACTTCCCGCGCCGGCCTCGACAACCCGAGCAAGCCGATCGGCGTGTTCATGCTCGCCGGC
ACCTCCGGGGTGGGCAAGACCGAGACCGCACTGGCCTTGGCCGAGGCCATGTACGGCGGCGAGCAGAATGTCATCACCAT
CAACATGAGCGAGTTCCAGGAAGCCCACACCGTCTCCACCCTCAAGGGCGCCCCGCCCGGCTACATCGGCTACGGCGAGG
GTGGCGTGCTGACCGAGGCGGTGCGTCGTAAGCCCTACAGCGTGGTGCTGCTGGATGAAGTGGAGAAGGCCCACCCGGAT
GTCCACGAGATCTTCTTCCAGGTCTTCGACAAGGGCGTGATGGAGGACGGCGAAGGCCGGGTGATCGACTTCAAGAACAC
CCTGATCCTGCTCACCACCAACGCCGGCACCGAGATGATCGCCAGCCTCTGCGCCGATCCGGAACTGATGCCCGAGCCGG
AAGCCATCGCCAAGTCGCTGCGCGAACCGCTGCTGAAGATCTTCCCGCCGGCGCTGCTCGGCCGCCTGGTGACCATCCCC
TACTACCCGCTCAGCGACGACATGCTCAAGGCCATTTCGCGCCTGCAACTGGGGCGGATCAAGAAGCGCGTGGAGGCGAC
CCACAAGGTGCCGTTCGAGTTCGACGAGGGCGTCGTCGACCTGATCGTCTCGCGCTGCACCGAGACCGAGAGCGGCGGCC
GGATGATCGACGCGATCCTCACCAACACGCTGTTGCCGGACATGAGCCGCGAGTTCCTCACCCGCATGCTCGAAGGCAAG
CCGTTGGCCGGGGTACGTATCAGTAGCCGCGACAACCAGTTCCACTACGACTTCGCCGAGGCCGAGTGA